EMAGE mouse gene expression spatial database

EMAGE:17666

Hdac11 histone deacetylase 11 ( MGI:2385252)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005998. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005998_01	euxassay_005998_02	euxassay_005998_03	euxassay_005998_04

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euxassay_005998_10	euxassay_005998_11	euxassay_005998_12	euxassay_005998_13	euxassay_005998_14

euxassay_005998_15	euxassay_005998_16	euxassay_005998_17	euxassay_005998_18	euxassay_005998_19

euxassay_005998_20	euxassay_005998_21	euxassay_005998_22	euxassay_005998_23

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T125
Entity Detected:	Hdac11, histone deacetylase 11 ( MGI:2385252)
Sequence:	sense strand is shown >T125 CCAGAGCAGGCAGGGCCGGCTGCGGGATGCCTCACGCAACACAGCTGTACCAGCATGTACCAGAGAAACG CTGGCCCATCGTGTACTCACCACGTTACAACATCACCTTCATGGGCCTGGAGAAGCTGCACCCCTTTGAT GCTGGGAAATGGGGCAAGGTGATCAACTTCCTGAAAGAAGAGAAGCTGCTGTCCGATGGCATGCTGGTGG AGGCTCGGGAGGCCTCGGAGGAGGACCTGCTGGTGGTGCACACGAGGCGCTATCTCAACGAGCTGAAGTG GTCCTTTGTGGTGGCTACCATCACTGAGATCCCCCCGGTCATCTTTCTTCCCAACTTCCTTGTGCAGAGG AAGGTGCTGAGGCCCCTGCGGACCCAGACTGGAGGCACCATCATGGCAGGGAAGCTGGCTGTGGAGCGAG GCTGGGCCATCAATGTTGGTGGTGGCTTCCACCACTGCTCCAGTGACCGTGGTGGGGGCTTCTGTGCCTA TGCAGACATCACACTGGC
Notes:	The probe template was PCR amplified from IMAGE:615767 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:615767 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17665 same embryo
	EMAGE:17667 same embryo
	EurExpress:euxassay_005998 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source