EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17688

Mxra8 matrix-remodelling associated 8 ( MGI:1922011)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006017. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006017_01	euxassay_006017_02	euxassay_006017_03	euxassay_006017_04

euxassay_006017_05	euxassay_006017_06	euxassay_006017_07	euxassay_006017_08	euxassay_006017_09

euxassay_006017_10	euxassay_006017_11	euxassay_006017_12	euxassay_006017_13	euxassay_006017_14

euxassay_006017_15	euxassay_006017_16	euxassay_006017_17	euxassay_006017_18	euxassay_006017_19

euxassay_006017_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17688_wholemount_strong.wlz

17688_wholemount_moderate.wlz

17688_wholemount_weak.wlz

17688_wholemount_possible.wlz

17688_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17688_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 14 15 16 17 18 19 20

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 13 14 weak expression: see section 04 15 16

4th ventricle

moderate

regional

moderate expression: see section 01 02 03 05 06 07 08 09 10 11 13 14 weak expression: see section 04 15 16 17

aortic valve

strong

regional

strong expression: see section 11 12 not examined expression: see section 08 09

mitral valve

strong

regional

strong expression: see section 08 09 not examined expression: see section 11 12

pulmonary valve

strong

regional

strong expression: see section 11 12 not examined expression: see section 09

tricuspid valve

strong

regional

strong expression: see section 11 12

mandible

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20

maxilla

strong

regional

strong expression: see section 03 04 05 06 07 08 16 17 18 19 20

premaxilla

strong

regional

strong expression: see section 09 10 11 14 15

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 19 20

clavicle

strong

regional

strong expression: see section 06 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T288
Entity Detected:	Mxra8, matrix-remodelling associated 8 ( MGI:1922011)
Sequence:	sense strand is shown >T288 GCCCAGACCCGGCCCAGGGCTCCGTGATGTCAGCGGGTGCTGGCAGGGAGAGGGGGTCGGCTGTTTTTCT GGAGAGTTAGAGCTGAGTAAGACAAAGCACGTCCCCCGCAGGCGCCATGGAACTGCTGTCCCGTGTCCTG CTGTGGAAACTGCTGCTTCTTCAGAGCTCTGCAGTCCTGTCCTCAGGGCCTTCAGGGACCGCAGCAGCCA GCAACTCTCTGGTGTCTGAGTCTGTGGTGAGCTTGGCAGCCGGAACCCAGGCTGTGCTACGCTGCCAGAG CCCCCGCATGGTGTGGACCCAAGACCGGCTGCATGATCGCCAGCGCGTGGTCCACTGGGACCTCAGCGGG GGCCCGGGCAGCCAACGGCGCCGACTTGTGGATATGTATTCGGCGGGTGAACAGCGCGTGTACGAGCCGC GCGATCGACCGCCTCCTGCTGTCGCCTTCTGCTTTCCACGACGGCAACTTCTCGCTGCTCATTCGCGCTG TGGAGAGAGGCGATGAAGGGGTGTACACCT
Notes:	The probe template was PCR amplified from IMAGE:2810909 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2810909 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17689 same embryo
	EMAGE:17690 same embryo
	EMAGE:17691 same embryo
	EurExpress:euxassay_006017 same experiment
	MGI:4826522 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source