EMAGE mouse gene expression spatial database

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EMAGE:17698

Elovl7 ELOVL family member 7, elongation of long chain fatty acids (yeast) ( MGI:1921809)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006035. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006035_01	euxassay_006035_02	euxassay_006035_03	euxassay_006035_04

euxassay_006035_05	euxassay_006035_06	euxassay_006035_07	euxassay_006035_08	euxassay_006035_09

euxassay_006035_10	euxassay_006035_11	euxassay_006035_12	euxassay_006035_13	euxassay_006035_14

euxassay_006035_15	euxassay_006035_16	euxassay_006035_17	euxassay_006035_18	euxassay_006035_19

euxassay_006035_20	euxassay_006035_21	euxassay_006035_22	euxassay_006035_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17698_wholemount_strong.wlz

17698_wholemount_moderate.wlz

17698_wholemount_weak.wlz

17698_wholemount_possible.wlz

17698_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17698_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 08 09 10 11 13 16 17 18 weak expression: see section 06 07 19

4th ventricle lateral recess

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 18 weak expression: see section 05 06 07 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T375
Entity Detected:	Elovl7, ELOVL family member 7, elongation of long chain fatty acids (yeast) ( MGI:1921809)
Sequence:	sense strand is shown >T375 GCAGGGGCCGCTGTGAGAGCTGCGCTCGCACGGCCCGCCGCCGCCCTCGGCTCCCGCGCGCGCTGCTCGC CCCTCGGCGCGATCTTAAGCTCATTTCTGCACTGTTTTACCGAAGAACCCATTAAAAGGGCCAGTGAATC TTGTGGAAAATGGCGTTCAGTGATCTTACATCGAGGACTGTGCGTTTTTATGATAATTGGATCAAAGATG CTGATCCGAGAGTTGAAGACTATCTCCTCATGTCCTCGCCTCTGCCACAAACCATCATCCTGGGCCTCTA TGTCTATTTTGTTACGTCTCTGGGACCAAAGCTCATGGAGAACCGGAAGCCCTTTGAACTCAAGAAAGCG ATGATAACGTACAATTTTTTCATAGTACTGTTTTCTGTGTATATGTGTTATGAGTTTGTGATGTCTGGCT GGGGTACAGGTTACTCGTTTCGATGTGACATTGTTGACTATTCTCAGTCGCCAAGAGCAATGAGGATGGT GCACACCTG
Notes:	The probe template was PCR amplified from IMAGE:3156207 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3156207 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17697 same embryo
	EMAGE:17699 same embryo
	EurExpress:euxassay_006035 same experiment
	MGI:4824534 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source