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EMAGE:17705

Brd8 bromodomain containing 8 ( MGI:1925906)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17705 EMAGE:17705 EMAGE:17705 EMAGE:17705 EMAGE:17705
euxassay_006036_01 euxassay_006036_02 euxassay_006036_03 euxassay_006036_04 euxassay_006036_05
EMAGE:17705 EMAGE:17705 EMAGE:17705 EMAGE:17705 EMAGE:17705
euxassay_006036_06 euxassay_006036_07 euxassay_006036_08 euxassay_006036_09 euxassay_006036_10
EMAGE:17705 EMAGE:17705 EMAGE:17705 EMAGE:17705 EMAGE:17705
euxassay_006036_11 euxassay_006036_12 euxassay_006036_13 euxassay_006036_14 euxassay_006036_15
EMAGE:17705 EMAGE:17705 EMAGE:17705 EMAGE:17705 EMAGE:17705
euxassay_006036_16 euxassay_006036_17 euxassay_006036_18 euxassay_006036_19 euxassay_006036_20
EMAGE:17705 EMAGE:17705
euxassay_006036_21 euxassay_006036_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17705Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17705_wholemount_strong.wlz
17705_wholemount_moderate.wlz
17705_wholemount_weak.wlz
17705_wholemount_possible.wlz
17705_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17705_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T324
Entity Detected:Brd8, bromodomain containing 8 ( MGI:1925906)
Sequence:sense strand is shown

>T324
AAGACGTCGAAGCCAAGATGGCGACCGGGACAGGCACCTTCTGTATTTCCAGAGCACAAGCTGCTGAGTA
CTGGCCCAACAGAGCCATGGTCCATCCGAGAGAAGTTGTGTTTAGCATCTTCTGTCATGAGGAGTGGGGA
TCAGAACTGGGTATCAGTTAGCAGAGCAATCAAGCCCTTTGCAGAACCTGGCCGGCCTCCAGACTGGTTC
TCTCAAAAACATTGTGCTTCTCAGTATTCTGAGCTCTTAGAGACTACTGAGACTCCAAAACGGAAACGGG
GTGAAAAAGGAGAAGTGGTAGAAACTGTTGAAGATGTTATTGTTCGCAAACTGACTGCTGAGAGAGTTGA
AGAACTGAAGAAAGTCATAAAGGAGACACAGGAGAGATACAGGCGTCTGAAAAGAGATGCAGAACTAATC
CAAGCCGGGCACATGGACAGCAGACTGGATGAGCTCTGCAATGACATTGCAATGAAAAAGAAATTGGAAG
AGGAAGAGGCTGAAGTAAAAAG
Notes:The probe template was PCR amplified from IMAGE:3154097 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3154097 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17702 same embryo
 EMAGE:17701 same embryo
 EMAGE:17700 same embryo
 EMAGE:17704 same embryo
 EMAGE:17703 same embryo
 EurExpress:euxassay_006036 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS