EMAGE mouse gene expression spatial database

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EMAGE:17712

Rnf112 ring finger protein 112 ( MGI:106611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006072. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006072_01	euxassay_006072_02	euxassay_006072_03	euxassay_006072_04

euxassay_006072_05	euxassay_006072_06	euxassay_006072_07	euxassay_006072_08	euxassay_006072_09

euxassay_006072_10	euxassay_006072_11	euxassay_006072_12	euxassay_006072_13	euxassay_006072_14

euxassay_006072_15	euxassay_006072_16	euxassay_006072_17	euxassay_006072_18	euxassay_006072_19

euxassay_006072_20	euxassay_006072_21	euxassay_006072_22	euxassay_006072_23	euxassay_006072_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17712_wholemount_strong.wlz

17712_wholemount_moderate.wlz

17712_wholemount_weak.wlz

17712_wholemount_possible.wlz

17712_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17712_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4210
Entity Detected:	Rnf112, ring finger protein 112 ( MGI:106611)
Sequence:	sense strand is shown >T4210 TCTCTGAGCATCGGACCCTCTGCCATTCCAACCTCTCATGCCGAGGCCCGTCCTGTCAGTCACTGCTTTT TGTCATCGGCTTGGCAAACGGGAGAGCAAACGAAGCTTCATGGGAAACAGCAGCAACAGTTGGGTTCTCC CCAGGGAGGAAGCACAAGGCTGGATGGGCCAGGCTGTGCAGGGAGGGACCAGGACAAGCAGGTCCCATGC ATCATTCCCCAAGCTGGAGCTGGGCCTGGGACACCGTCCCTCCCCAACCCGGGAGCCGCCCACCTGCTCC ATCTGTCTGGAAAGGCTTCGAGAGCCCATCTCGCTGGACTGTGGCCATGACTTCTGCATCCGATGCTTCA GCACACACCGCATCCCAGGCTGTGAGCTACCATGCTGTCCTGAATGCCGGAAGATCTGTAAGCAAAGGAA GGGCCTCCGCAGTCTAGGGGAAAGGATGAAACTCCTACCACAGCGGCCACTGCCCCCTGCACTGCAGGAG ACCTGTGCTGTGAGGGCAGAACGTCTGCTGTTGGTACGAATCAATGCCTCCGGAGGCCTCATCCTCAGGA TGGGCGCCATAAACCGTTGCCTGAAGCATCCACTGGCCAGGGACACACCCGTCTGCTTATTGGCTGTCCT GGGAGAGCAGCACTCAGGAAAGTCCTTCCTTTTGGACCACTTGCTCAGTGGCTTACCAAGCCTGGAATCT GGTGACAGCGGCAGGCCCAGAGCAGAGGGGTCTCTGCCTGGGATCAGATGGGGCGCTAACGGCCTCACGA GGGGTATCTGGATGTGGAGTCACCCCTTCCTGCTGGGAAAAGAGGGGAAGAAGGTGGCTGTGTTCTTAGT GGACACGGGGGATGTCATGAGCCCAGAACTGAGCAAGGAGACAAGGGTCAAGCTCTGTGCTCTCACCATG ATGCTCAGTTCCTACCAGATCCTGAATACCTCCCAAGAGCTGAAGGATACCGATCTGGGCTATCTAGAGA TGTTCGTTCACGTGGCTGAGGTGATGGGCAAACATTATGGGATGGTACCAATCCAGCATCTGGATCTCTT AGTCCGAGACTCTTCCCATCATAATAAGTCAGGGCAAGGGCACGTGGGTGACATACTCCAGAAGCTGTCC GGCAAATACCCCAAGGTCCAGGAGCTGCTCCTCGGGAAACGGGCCCGCTGTTACCTCCTCCCTGCTCCTG AGAGGCAGTGGGTAAACAAAGACCAAGCCAGCCCCAGAGGCAACACAGAAGATGACTTCTCCCACCATTT CCGGGCCTACATCTTGGACGTGCTGAGCACAGCCCCTCAGCACGCTAAGAGCCGCTGTCAAGGGTACTGG AGTGAGGGGCGTGCCGTGGCCAGGGGAGACAGACGCCTGCTCACAGGGCAGCAGCTAGCACAGGAGATCA AGAATCTCTCTGGCTGGATGGGGAAGACTGGGCCCAGTTTCAACTCTCCTGATGAGATGGCTGCTCAGCT CCATGATCTGAGGAAAGTGGAAGCCGCCAAGAAGGAGTTCGAGGAGTACGTGAGACAGCAGGACATAGCC ACCAAGCGCATCTTCTCTGCACTACGAGTCCTGCCTGACACGATGAGGAACCTCCTCTCTACCCAGAAGG ATGCCATCTTGGCCCGCCATGGTGTGGCCCTGTTGTGCAAGGAGAGAGAGCAGACCTTGGAGGCCCTGGA AGCCGAGCTGCAGGCAGAAGCCAAGGCCTTCATGGACTCCTACACAATGCGCTTCTGTGGCCACCTGGCC GCGGTAGGGGGCGCTGTAGGTGCTGGGCTCATGGGCCTGGCAGGGGGTGTGGTGGGCGCAGGCATGGCAG CAGCGGCGTTGGCTGCTGAGGCTGGGATGGTAGCAGCTGGGGCCGCGGTGGGGGCCACCGGGGCTGCTGT GGTTGGGGGTGGTGTGGGTGCTGGTCTGGCCGCCACTGTGGGCTGCATGGAGAAAGAGGAAGATGAGAGA GTTCAAGGAGGGGACCGAGAGCCCCTACTCCAGGAGGAATAGCAGCAAGATGTCGAAGCGTGAAATACGT GAAAGGCAGTAGGAAGGGTGCGGACATCAGGGACTCAGAGGGGACCACGTATATACTGGCCTTGTTGAAT GCCCCATGTCAAACACTGGCTGAGCTGGGGGTTTACGGTGGGTCCAGAAACCTGAGGAGGCTTTCTGGCC ACGAGGCTGTACTTGGAGTACAGCGGATAGGGTGCAAGGCCTGTTTCTGGCTCTGGATAATTGCTACTCT CTGGAACCTAGGATCTCCCACCACATAAGGCCCCACCTCCTCTACCCAGTTCCAGTTTATTTCCCTGGAG GGCCTGGGCTGGAAATTTGCCCCTGGATCCAGAACCCCATTCGTTTTACAGTTGGGATAGGTCCCAGCAT AACTGACTTTGGAGCTAGGAGCCCCGCTAACAGGGAAGAAAAAGGAGGGGTCACAGAGCAGGGATGGGGA TGATAGACAGAGCTATAATTCTGGAGTGGTGACTTGTTTCCAAAACTGCTCCAGAAGGCCAGGCTTGGCT GGCCTCCCTGCTCTTCAGTTGGGTGTCCAGATGTGTGTCCTGAACCGCCCTGTACCCCAGCCTCCTGCAG TCCTCCTAAGCCTGTGCCCTGTGCCAAGCCCATTTGGATGGATCAACACAGATTACATGCCAAAGAACCA CACCTCCGATTGCATTCTGTGCTAAAGCAGTCCGTGCCAGGTTCTGCCAAAGTGTGGGAAGGGAAAGTGC AGGAAACGAGCTCCGTCTTTTAACATAAGCTTGAGCTGTGGGGCCTCTGCGCCACACTGTAAGACCCCCT TGATGCAGCCCTCTGAACGCCAAGCCTCCAGAAAGCTTGTGGGCAGGGGGCCTCTCTCGGGGGATGGAGA TTTCTAAAGAGACTTCTGGGCTGTTAAACTATAAAAGATGTTGGGCATATGACCCTTTATTTTATATAAA AATAAAATGTGTGCGTGTGAATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4500921 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4500921 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17714 same embryo
	EMAGE:17717 same embryo
	EMAGE:17713 same embryo
	EMAGE:17716 same embryo
	EMAGE:17715 same embryo
	EurExpress:euxassay_006072 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source