EMAGE mouse gene expression spatial database

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EMAGE:17714

Crybb3 crystallin, beta B3 ( MGI:102717)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006066_01	euxassay_006066_02	euxassay_006066_03	euxassay_006066_04	euxassay_006066_05

euxassay_006066_06	euxassay_006066_07	euxassay_006066_08	euxassay_006066_09	euxassay_006066_10

euxassay_006066_11	euxassay_006066_12	euxassay_006066_13	euxassay_006066_14	euxassay_006066_15

euxassay_006066_16	euxassay_006066_17	euxassay_006066_18	euxassay_006066_19	euxassay_006066_20

euxassay_006066_21	euxassay_006066_22	euxassay_006066_23	euxassay_006066_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17714_wholemount_strong.wlz

17714_wholemount_moderate.wlz

17714_wholemount_weak.wlz

17714_wholemount_possible.wlz

17714_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17714_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4220
Entity Detected:	Crybb3, crystallin, beta B3 ( MGI:102717)
Sequence:	sense strand is shown >T4220 CGGACGCGTGGGGGAAGCAGTGCTGTTTGGGTGGGAACCCACCAGTCCCTGATCCTGCTGGCCGCAGCTG GTGACCTGGTTCTCCTCCAGTTTGAGGCAGAAGTATCCCCAGAGAGATGGCGGAGCAGCACGGAGCACCT GAGCAGGCTGCAGCTGGCAAGAGCCATGGAGGCCTTGGGGGCAGCTACAAGGTGACGGTGTACGAGTTAG AGAACTTCCAGGGCAAGCGATGCGAGCTCTCGGCGGAGTGTCCCAACCTCACCGACAGCCTGTTGGAGAA GGTGGGCTCCATCCAAGTGGAGTCTGGACCGTGGCTGGCGTTCGAGCGCAGGGCCTTTCGTGGGGAGCAG TTTGTTCTGGAGAAGGGGGACTACCCTCGCTGGGATGCCTGGTCCAGCAGCCGACGTAGTGACATTCTCC TGTCCCTCCGTCCTTTGCACATTGATGGCCCAGACCACAAGCTGCACCTGTTTGAGAACCCAGCCTTCAG TGGGCGCAAGATGGAGATCGTAGATGACGATGTGCCCAGCCTGTGGGCCCATGGTTTCCAGGACCGTGTG GCCAGCATCCGTGTCATCAACGGGACGTGGGTGGGCTACGAGTTCCCGGGCTACCGTGGGCGCCAGTACG TGTTCGAGCGGGGCGAATTCCGCCACTGGAACGAGTGGGACGCCAACCAGCCGCAGCTGCAGTCGGTGCG CCGCATCCGCGACCAGAAGTGGCACAAGCGCGGCTGCTTCCTGAGCAGCTGAGGACTCCCGGGAGCCAAC GCCTGTGTCCCCTTCCTGAACCTGCTCAATAAAGCCCACCAGCCACGCAAAAAAAAAAAAAAAAAAAAAA AAAAATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4501690 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4501690 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17717 same embryo
	EMAGE:17713 same embryo
	EMAGE:17716 same embryo
	EMAGE:17715 same embryo
	EMAGE:17712 same embryo
	EurExpress:euxassay_006066 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source