EMAGE mouse gene expression spatial database

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EMAGE:17715

Cdk17 cyclin-dependent kinase 17 ( MGI:97517)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006070. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006070_01	euxassay_006070_02	euxassay_006070_03	euxassay_006070_04

euxassay_006070_05	euxassay_006070_06	euxassay_006070_07	euxassay_006070_08	euxassay_006070_09

euxassay_006070_10	euxassay_006070_11	euxassay_006070_12	euxassay_006070_13	euxassay_006070_14

euxassay_006070_15	euxassay_006070_16	euxassay_006070_17	euxassay_006070_18	euxassay_006070_19

euxassay_006070_20	euxassay_006070_21	euxassay_006070_22	euxassay_006070_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17715_wholemount_strong.wlz

17715_wholemount_moderate.wlz

17715_wholemount_weak.wlz

17715_wholemount_possible.wlz

17715_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17715_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4208
Entity Detected:	Cdk17, cyclin-dependent kinase 17 ( MGI:97517)
Sequence:	sense strand is shown >T4208 AGCAGTAGCAAGGACAACGAGCCCATTGTGAAGAATGGCAGGCCGCCCACGTCTCACAGCGTGCATTCCT TCCTCCATCAGTACACAGGCTCCTTCAAGAAGCCCCCGTTGCGGAGACCCCACAGTGTTATTGGAGGAAG CCTTGGTTCCTTCATGGCAATGCCCAGGAATGGAAGCAGACTAGATATTGTTCATGAAAATCTGAAGATG GGATCAGATGGTGAGAGTGACCAAGCTTCTGGGACATCATCTGATGAAGTCCAGTCACCTACAGGTGTTT GCCTCAGAAATCGTATACATAGACGGATCTCAATGGAGGACTTGAATAAGCGGCTGTCGCTGCCTGCAGA CATCAGGATACCTGATGGCTACCTGGAGAAGCTGCAGATCAGCAGCCCACCCTTTGACCAGCCCATGAGC CGCAGGTCCCGCAGAGCTTCCTTATCAGAAATTGGGTTTGGGAAAATGGAAACCTACATCAAATTGGAAA AGCTCGGAGAGGGTACATATGCAACAGTATATAAAGGAAGAAGTAAACTGACAGAAAATTTGGTGGCATT AAAAGAGATCCGCTTGGAACACGAAGAAGGTGCACCCTGTACAGCTATAAGAGAAGTTTCACTGTTAAAG GATTTAAAACATGCAAATATAGTAACCTTGCACGACATCGTTCACACAGACAAATCTTTGACTCTGGTCT TTGAGTACCTGGACAAAGACCTGAAGCAATACATGGATGACTGTGGGAACATCATGAGCATGCACAATGT CAAGCTGTTCCTGTACCAAATTCTCCGTGGTCTGGCATATTGCCATAGAAGAAAAGTGTTGCACCGGGAC TTGAAACCACAGAACCTCCTCATTAATGAGAGAGGAGAACTAAAGCTGGCTGACTTTGGACTAGCCAGAG CCAAGTCAGTTCCCACAAAGACCTACTCAAATGAAGTTGTCACCCTGTGGTATCGACCACCTGATGTGCT TCTTGGTTCCTCAGAATACTCAACACAGATCGACATGTGGGGAGTTGGCTGTATTTTCTTTGAAATGGCT TCTGGAAGACCACTGTTTCCAGGGTCAACTGTGGAAGATGAACTGCATTTAATATTTCGACTACTAGGAA CTCCATCTCAGGAAACTTGGCCAGGTGTTTCTTCAAATGATGAATTCAAGAACTACAACTTCCCAAAATA CAAGCCACAACCTCTAATTAATCATGCACCCAGGTTAGATTCTGAAGGAATTGAATTGATAACAAAATTT CTTCAGTATGAATCTAAGAAGAGGGTTCCGGCAGAAGAGGCCATGAAGCACGTGTACTTCCGCAGCCTGG GGCCGAGGATCCATGCTTTGCCCGAGAGCGTATCAATATTCAGTTTGAAAGAGATTCAGTTGCAAAAGGA CCCGGGTTTTCGAAACTCTTCTTATCCAGAGACAGCCTTATCCTGATCCTGGAAACTGCAGAGAGGAGAC TTAAAACTTGGAAAGACTGAACCTGGAACTCTCAGAAGCCCCTTAAACTGACAAGAATTCTGAAGGGGGA CTGTCATCACAGGGCTCCTCCAGCTTCTCCGTCTTGATGGCCTTGAAGGCCTCTGGAGCGTTCTGCAGGG AGTTGATGGTGAAGGACGAGTAGAGGCCCGAGTGGAGGTACTCGTTGCGGCTGGCACTTTTGAGAGAGGA CAAGCCGTGCCTGTGGACCTCTCGGCCTTCCGGGGACACCTTACAGTCGCCGTCCTGCAGCAGGAGGCTC TCCCGGCTGATCTCTACCGCGTGAGGATCCATTTTCAGGATATCCGGGAAAGAGACGAACTTGTACACAA ACTTCTGCCCGATCACTTTCTTGATGATGTTCTGTGAATGCAAAACAAAAAACAAAACAAAAAACAAAAA AAAAACCCAGACATTTAGAAGGGGTGAGCCAGTGACATGGAAGGAGGGGGCCCCTGGAACCACAAATGAT AGCAGCCAAAGCGCCACCATATTCCTGATGCCCACAGGGTGCCTACAAGGAGTGACGTCAGAGACTGAAG GTTCACTGTTACACTCTGGTCCTGTTGACCCAGGAGGAGGGGTGAGGTCTCCAGCCTGTGGTGCCTTTCT CATCTATTGTCTGTGACGATTTTGTAATAGCAAAAGCTACCACTGAGCTAGCACTTAACTGCAGGGCTGG GATGATTCAGACCGTAGCACATGTCATTTGCACAGTAATCCTACAAGCAGGCTTTATCGTTCTTGTTGTG CAATTAGAAGAGAGAGCTGGGGAGAGCGCCTAGCGAATAGATCACATGCATGAGGCCCGCGAACTTCGGA TCATCAGCACCCATGTAAAAGGGGGGAGGGGGATGGATGTCTAGGACAAGCTAGATATCTAGGATGGCTA GCTAGACCATTCTGAATCCATGAGCTCTGGGTTCAGCAAGGGTTCCTGTCTCAGCAAGTAAAGTGGAGAG TGAGTGAGGAAGACATCTGATGCCAGTCTTGTGGCTACACACACATGGCCAAGTGTGTACCCATGTACCA CGTATACCCACACATGCGTGCACGCCACATAACACATGCAAAAAGAAAAATTCTAAGAAGTAGGAGATAA ACTTTGAGTACTGCAGTGACGCACATGGCCAAGGTTAAGCAAGAAGGGCCAAGGGAGATTGACTTCACGC TAACACGGGACTGTCTTACCCGCCTCTGGGAACCGAGAGACTACAGTGAAATACTAACAAGCAAGCACTC TGCTAGAGAACATCTGGGAAATGTAAGTTACCGCCACCCAGCAACGCAACCAAGGTTTGCTTCAGCCAGG CAGCCATTTCTGGCATGAAAGCCACTGGAGAAAACGAAGTCGCCTGCTTGGTTATTTATGGCATTTGGTT CATATTCTTAGTTGCTGGTGTCTTCTTCTGGCTCTTCAGTTAAGTCCTCATCCCTCCAGCAGCATGATAT AAATGGAACATGAAAATCGCTATGAAGGCAAGAGTTTTCTCAGCCCATGAGAAAGTGGGTCTCACGTGGC AGGCTATGCTGTTCTTAAGGGTAATTGAGCTGTGGGAGTAGTCCCTGTAGAGCCTACCTAGTTCTAGGTC TGTCTGGCAAATGCTTGCTACAGGTCTCACCCTATGGTTGACTCGGAGTCCTGGGTACTCTGGGGAGAAA AACATGGCTTCCCTCTGGCTCAAAAAGCCAGCATTATTCTATGAGAAAGATCCCTGTAGGCTTCTCTACA AACTCATCCTTCTAAAGTTTGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4501115 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4501115 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17714 same embryo
	EMAGE:17717 same embryo
	EMAGE:17713 same embryo
	EMAGE:17716 same embryo
	EMAGE:17712 same embryo
	EurExpress:euxassay_006070 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source