EMAGE mouse gene expression spatial database

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EMAGE:17716

Gyg glycogenin ( MGI:1351614)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006069. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006069_01	euxassay_006069_02	euxassay_006069_03	euxassay_006069_04

euxassay_006069_05	euxassay_006069_06	euxassay_006069_07	euxassay_006069_08	euxassay_006069_09

euxassay_006069_10	euxassay_006069_11	euxassay_006069_12	euxassay_006069_13	euxassay_006069_14

euxassay_006069_15	euxassay_006069_16	euxassay_006069_17	euxassay_006069_18	euxassay_006069_19

euxassay_006069_20	euxassay_006069_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17716_wholemount_strong.wlz

17716_wholemount_moderate.wlz

17716_wholemount_weak.wlz

17716_wholemount_possible.wlz

17716_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17716_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart atrium

moderate

regional

moderate expression: see section 02 03 04 05 06 07 12 13 14 15 16 17 18 19

heart ventricle

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15

left lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

right lung

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4190
Entity Detected:	Gyg, glycogenin ( MGI:1351614)
Sequence:	sense strand is shown >T4190 GCGGACTCCCGCCCTCCCTGCTGGCCGCGGTCACCTACCGGCGCCCGGCTCCTCCGACCCACCCGGCCAC ACTATGACAGATCAGGCTTTTGTGACGCTGACCACAAACGATGCCTATGCCAAAGGTGCGCTGGTCCTGG GCTCATCTTTGAAACAGCACAGGACTACTAGGAGGATGGTTGTACTCACCAGCCCACAGGTTTCAGACTC CATGAGAAAAGTTTTAGAGACTGTCTTTGATGACGTCATCATGGTAGATGTCTTGGACAGTGGTGATTCT GCTCATCTAACCTTAATGAAGAGACCTGAGTTGGGCATCACATTGACTAAACTCCACTGCTGGTCACTTA CTCAGTATTCCAAATGTGTGTTCATGGATGCAGATACTCTGGTCCTCTCAAATATTGATGATCTGTTTGA AAGAGAAGAATTATCAGCAGCACCAGACCCAGGGTGGCCTGACTGTTTCAATTCTGGAGTCTTTGTCTAT CAACCCTCCATTGAAACGTATAACCAGCTGTTGCATCTGGCTTCTGAGCAAGGTAGTTTTGATGGTGGGG ACCAGGGCCTACTGAATACATATTTTAGTGGCTGGGCAACGACAGATATCACAAAACACCTGCCATTTGT GTATAACCTAAGCAGCATTTCAATATACTCCTACCTCCCGGCATTTAAAGCGTTTGGTAAAAATGCCAAA GTTGTGCACTTCCTGGGACGAACCAAGCCATGGAATTACACTTATAACCCCCAAACAAAAAGTGTCAACT GTGACTCCCAGGACCCAACCGTGAGTCACCCAGAGTTTCTGAACCTGTGGTGGGACACCTTCACCACCAA CGTCTTACCCCTGCTCCAACACCATGGCCTTGTCAAAGATGCCAGCTCCTATCTAATGATGGAACATGTC TCAGGAGCCTTGTCAGACCTGTCCTTTGGGGAGGCCCCAGCTGCACCACAACCTTCTATGTCCTCAGAAG AAAGGAAGGAACGGTGGGAACAGGGCCAGGCCGATTACATGGGAGCAGATTCTTTTGATAACATCAAGAG AAAACTTGACACTTACCTGCAATAGAAGCACTGCCATTTTCCTGTGGACACACCCATGTCTTAGGCACGT TTCCATACATAGTATGTGAAGTCTGTTACAGTTGTTAGAGGCTTTCACTAAACCTCATCAGATGAGGGGT TTGGGCAGGACAACAGGTGAGAACTGGGCAAATGTTATTAGGTCGTAATTCCATCATGTGGACAAGTGTT CTGTTTAACCCTAGACTTTTTTTTTTTAATTCTCAATTCTTAAATTGCCAATGTGTGATAAAAGAAAGCC AACATTAAGCTACTAAGATGGCTCTCTCAAGTGGTGCATAACTTGACTCTCCATCTTCAAAACAAGGTGT GACATTTTCCAATCTTCACTTGTTGCATCCTTCACAAGACCTGCAATGTAGAACTGCTCAAAGCCTGTCA GAGAAGTAGCCTTCCAGACTAAAGTGGCCTTTATTAACACTGCGGAGTGATTCCTAAGCCTACCAGCTAG TGTTAGTTCACCAGTTTTCTATGCACCGTGAAGGCAGCATGCAGACTTGTTCTTTCTCAGTTGTCTACTT GAAAATTGCAGTGTGCTCTCTGATGGTAGCTGTGGTAGCACTGTGTATAACAAATAAACACTTATTGACA TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4487769 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4487769 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17714 same embryo
	EMAGE:17717 same embryo
	EMAGE:17713 same embryo
	EMAGE:17715 same embryo
	EMAGE:17712 same embryo
	EurExpress:euxassay_006069 same experiment
	MGI:4825290 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source