EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17721

Phactr4 phosphatase and actin regulator 4 ( MGI:2140327)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006071. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006071_01	euxassay_006071_02	euxassay_006071_03	euxassay_006071_04

euxassay_006071_05	euxassay_006071_06	euxassay_006071_07	euxassay_006071_08	euxassay_006071_09

euxassay_006071_10	euxassay_006071_11	euxassay_006071_12	euxassay_006071_13	euxassay_006071_14

euxassay_006071_15	euxassay_006071_16	euxassay_006071_17	euxassay_006071_18	euxassay_006071_19

euxassay_006071_20	euxassay_006071_21	euxassay_006071_22	euxassay_006071_23	euxassay_006071_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17721_wholemount_strong.wlz

17721_wholemount_moderate.wlz

17721_wholemount_weak.wlz

17721_wholemount_possible.wlz

17721_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17721_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9542
Entity Detected:	Phactr4, phosphatase and actin regulator 4 ( MGI:2140327)
Sequence:	sense strand is shown >T9542 CAGAGACCAGGTAGGATTTCCGGGAGAAAGTGCTGGGGAACCCGAGGAGGCAGCGGCGACTTGGAAACTA CTCCTTCTCTCCCTACACTGGTTAGCAGTGGCATGGAAGACCCATCGGAAGAAGCAGAGCAGCCCTCTGG AGACCCAGGCATGGGCATGGACAGTGTGGAAGCAGGGGACACAACACCTCCCACCAAAAGGAAAAGCAAG TTCTCAGCGTTAGGCAAGATCTTCAAGCCGTGGAAATGGAGGAAAAAAAAAAGCAGTGATAAGTTCAAAG AGACATCAGAAGTTTTAGAGCGAAAAATATCTATGCGAAAACCAAGAGAAGAACTGGTTAAACGAGGGGT TCTGTTGGAGGACCCCGAGCAGGATGGTGAGGATTCAGGAAAGCTGAGCCATGCTGCATTAAAGAACGGA CACACCACCCCCATAGGCAGTGCCAGGTCCTCTAGCCCAGTCCTGGTAGAAGAAGAGCCAGAGAGAAGTC TCAGGAACCTCACTCCAGAAGAGGAGTCAAAGAAGAGACTTGGCTCAACTGGAAGCCAGCCTAACTCTGA AGCCGAGCCTGGTCCTGAGCATGCACCTAAGCAGCCTCTGCTGCCCCCCAAGAGACCCTTGTCCTCTTCT TGTGAAGCAAAGGAGGTTCCGGCTGGCAGTACAGCAAGGTCCGTCTCCTCCACCTCTGGCTCTACCACCG TGACATCTGCTGCCACCACTGCTGCCACAGACATGACAAAGACTGTTAAGTCCTTTGTGGGCCCCACCCC CGCTCCTGCCCCCGCCCCCAGGACTCTGCCTGCTGCTCCTGCCAGTGCTAACACTGCTGCCACCACTACA GCCCCTGCCAAGCAACCCCCCATCCCACCCCCTAAACCAGCTCAGAGAAACAGCAACCCCATCATTGCTG AACTATCCCAAGCAATGAACAGTGGCACAGTGCTGTCGAAACCATCCCCACCCTTGCCACCCAAGAGAGG CATTCCATCAACTTCGATACCCAGCTTGGAGCCTGCTGCTTCGTTCACAACTAAGACAGCAAATGATCAA AGAGAAAAAACTGTCTCTTTGTGTTTGGAACCACCACTGATAATCCCGCCTTCGTCCCCATCACCCCCAC TGCCTACCCACATACCTCCCGAGCCTCCACGGTCCCCTCTAGTGCCTGCTAAGACTTTTCAAATTGTGCC AGAGGTTGAGTTTTCCTCTTCTTCAGATCTATTCCAGGACATTTCCCAGCAGGAGGATCAGAAAACAGAA GTCCCCAAGAAGATACAGGATCAGAGCTTTGGGGAGTCCCATATACCCTCCAGGCTGCCCCCACTCCCAC TGCATATCCGAATTCAGCAGGCCCTTACCAGCCCCCTTCCTGTGACCCCTCCCTTGGAAGGCACTCACAG AGCCCATTCACTGCTTTTTGAGAACAGTGACAGCTTCTCCGAGGACACTGGTACCCTGGGTCGTACCAGG TCACTTCCCATCACGATTGAGATGTTGAAAGTTCCAGATGATGAAGAGGAAGAGCAGACCTGCCCGTTTG TTGAAGACGTGACGTCTACCTCAGCCACTCCTAGCCTACCACTGTGTCTGCGAGAGGAAGAGAAGGAGAG TGACTCAGATTCAGAGGGTCCCATTAAGTACCGAGATGAAGAAGAAGATGATGATGATGATGAAAGCCAT CAGAGTGCCCTGGCCAACAGAGTGAAGAGGAAGGACACGCTGGCAATGAAGCTGAGCAGCAGACCCAGTG AACCAGAGACCAACCTGAATTCTTGGCCTCGTAAAAGCAAGGAGGAGTGGAATGAAATACGGCACCAGAT CGGGAACACGCTGATCCGGCGGCTGAGTCAGAGACCAACAGCAGAAGAATTGGAGCAGCGGAACATACTA CAGCCTAAAAATGAAGCTGATCGGCAAGCAGAGAAACGGGAAATCAAGCGTCGCCTCACTAGAAAGCTCA GTCAAAGGCCAACTGTGGCTGAACTGCTTGCCAGGAAGATTCTGCGGTTTAATGAGTATGTGGAAGTAAC TGATGCTCATGACTATGACCGGCGAGCAGACAAACCATGGACCAAACTGACTCCTGCTGACAAGGCTGCC ATAAGGAAAGAATTAAATGAATTTAAGAGCTCGGAGATGGAGGTTCATGTAGACAGCAAACACTTTACAC GCTACCATCGCCCATGAAGCTGAAGCCTGAGAAAGGAGCTAAAGGACCACAGGAAAAGGAAAAGCATGGC TGGTCTGCTTCTTGTCTCCAGGGCGAGCACAGCACTTCTCACACAGCCAGAGCTGCTCTGGGATCTGGGC AGGGCAGGCAGCGCTTGTACGCGTGTCCTTGTGCTCCCCTTAGAGAAGAATGAGACACCGATGCTTTGAG CCTTTTAACAACACTGCAGTAGACTTGAGGGTGTCTCTCACCAGCCACAGAAGCACAGGGCCTCTTACAG GTTTTGAGTTTTTCTGGCTACGGCACACGCTGCCCTAAGACAACTTGGTGTGCTGAGGCCGTTTGTACCG TGAGCCTTACCTGTCTGTCCTGTCTGAGAGCCGCTCAGGGAGGCTCATTCCAACCCTCAGGTTCTCTTAC CAGGAAAAAAAAAACATTCGGACACACCAGCCCTTCCAGCAAAGGCCCGCTTCCAGAAAAGTGCTTGGAC TTCTCTGCATAAGGTCTGACCAGAAGGAAGCCAGTGCAGGCTCCGGCCTGTTCCTCCCTCCATTTGGCAC TTGTCATCCTAGGTCCTTTTTGTTTTGAGACAAGATCTTATCCTGTGGCTCAGGCTGGCCTCAAACTCAC TGTGTAGCCCAGGCTGGCCTCAAACTTCTGTCTCAGCCTCCCAAGTGCTGAGATTATAAGCATGAGCCTT CATGCTCTCATCCTGATTTCAAAGTGAGCTTAATGAAATGGGGAAAATCCATTCATTATGTAGTCTGAGC TGTAATCAGAATCCAAATGATCAAATGAGAAAGCCACGTGCATTTTCTATGTACAAACCAAGAAAAAGGC CTTACTGTTAATGCCGGTTTGGCAGAGAACTGACTCACGGGGCAGACTTTATTATACGTTCCCTGTACCA AATGATACTATAAATCACTTTGGCCTGTGGGAGCACTGTGTGGGAGCACTGTGCGCTACAGGTGCCCATG CTCACTCCTTGGTGCTGTTGGCCCTCCTTACTGCACAGCCCCTCTACTGAATTAGCCCCTCCCCCTCCCG ACAACGAACACCTCACTTGCCTTCCCATTCCTGTTGCAGCCAGGCCTCCCCACTTGTATTGGGGTGCAGA TTTGGTTATGTTTTTCAAAGAACTTTTTTCCTGAAACCCACACAGCCTTACTGTTCCTGCCTGGATTCTA CTTACATTGTAAGTGGAAGGGAAATGTAACTGGGTTTGTCGCTTTTCTTTTTGTTTTAGTCTATACCTCA GTATGTAGCCCAGGCTAGCCTTTGACTTGAAGTCTTCCTTACTCAGCCTTCCCAGTCTGCGATCATCGAG GGCAACGGCCACACCCAGCTTCTACTCACCTTAACAGCTAGTGGATTGTGGCTCTTAAAAAGGCTTTGCT TTCTTGTTTGTTTGTTTTTTGCTTTATTCTATTTCCTGTCTGTATTCTTTCAGCAATTTTCTCCTTGTCA GCATCACACTTATTAAAGAGTTAGAATATAGGGCAAACAAAAAAAGCTAAATTGACCTTAAAACTAGTAC TTTCTTCTCTATGAAGGAAAAAGTGCTTTTGTTTGTTTTTTCTCTTCCTGCCATGTAGTTTCTGTCTATG GAACAAAGACTCCTTCCCACTCCGTCCCCATCTCCTCAAACCTTGATATTGCTTGAGTAAAGGAAGAGAA AACAACAGTTCTGTCAAGTCTGTGAGTTGTCTAGATACAAGGTTGCCAGAGCTGCTAACCTTTGCGATTA CATCCTGACCAAGGCTCAGTTCTCACAAGCAAGGCCGGTGCTCCGCCCCTGCTCCGCCCCTGCTCCGCCC TGCTCCACCCTGCTCCGCCCCGTTTCTCCTTCTCTGTCCCAGGTTTAGAGAGTACTTTGCATATGCCTGT GCTGTCTGGGGTGTGGTAACCTTTTTTTTAGACAGGGTCTTATGTAGCCTCACTATGTAGCCAAGGATCC CCTGCCTCCACCTCCCGAGTACAGGGCTCACAGATGCACCCACCATACCTGCCGGCCAGCAACGGCTTCT CTCAGCTGTTCTCCCTCACGTGCCAATCCTGAGCTCCCGTCTGCTTGTGTGTGCCATCAAGGGCTCTGAC TCTGTTCATCTCATCTGTCTTCTGAAGCAGGATATGTTAGGGCTGCATCTAGGCAGTGGTTTTGTTTTTA TGTATATAAAAGTTGATTATGTGTCTTCTTTCTGTAAGTGGAAGCTTACAGTCCTAATGTAAAGGACTTA AAGCCTCGCTGTCGCATGTTAAGATGCTTCCCTACTGTACAGAATCCTGTAAAATACATAATTACGTTGT ATATCAGCCTTCCTACCAATCTTAATTATTAAAGTATTTTAGAATATCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30549496 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30549496 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17719 same embryo
	EMAGE:17718 same embryo
	EMAGE:17720 same embryo
	EurExpress:euxassay_006071 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source