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EMAGE:17754

Fem1b feminization 1 homolog b (C. elegans) ( MGI:1335087)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17754 EMAGE:17754 EMAGE:17754 EMAGE:17754 EMAGE:17754
"Pseudo-wholemount" of euxassay_008916. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008916_01 euxassay_008916_02 euxassay_008916_03 euxassay_008916_04
EMAGE:17754 EMAGE:17754 EMAGE:17754 EMAGE:17754 EMAGE:17754
euxassay_008916_05 euxassay_008916_06 euxassay_008916_07 euxassay_008916_08 euxassay_008916_09
EMAGE:17754 EMAGE:17754 EMAGE:17754 EMAGE:17754 EMAGE:17754
euxassay_008916_10 euxassay_008916_11 euxassay_008916_12 euxassay_008916_13 euxassay_008916_14
EMAGE:17754 EMAGE:17754 EMAGE:17754 EMAGE:17754 EMAGE:17754
euxassay_008916_15 euxassay_008916_16 euxassay_008916_17 euxassay_008916_18 euxassay_008916_19
EMAGE:17754 EMAGE:17754 EMAGE:17754 EMAGE:17754
euxassay_008916_20 euxassay_008916_21 euxassay_008916_22 euxassay_008916_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17754Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17754_wholemount_strong.wlz
17754_wholemount_moderate.wlz
17754_wholemount_weak.wlz
17754_wholemount_possible.wlz
17754_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17754_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35441
Entity Detected:Fem1b, feminization 1 homolog b (C. elegans) ( MGI:1335087)
Sequence:sense strand is shown

>T35441
AAGGGGTAGCTGACTCTGACACTGGCTTTTTCCAGTCCACTCTTGATTATAATCACAGCACAACCACCCA
GAGGTTCTTCACTGTGGGAACTTTAAACATGCAAACCAAAACAACCTGGCCCATCTGCCATGCTTTGGTT
TTGCTAGTTTCTGAAAGGAGTTTTGATTTCATTACACTAATAGTTGCTAAATGGTGATTATGAAGACTTA
GGCATGATAGCATTGTTTGACATAAACTGTGGTTAAGGGATTAAAACCTCCCTAAAGCCAACACTGTTTT
AGACAAACATTTCTGAAAACTCAAGTCCTAGAGAGGAGTTACTCATTGTAATTGAGGCATCAGGTTAAAA
TGAGGTTGCTTTGGATAATCCAAGCAGCAGAATACCCGTGTTCTGGGAATATGGAAGCCAAAGGACGCGT
GCTACTTGAACTCTGTGGGCTGAGAAGAAGCCAGTGGCTGAACTTGAGTGGGCGGGTTAAAGTATCTGTG
CCTCATGACAATTGTAAAAATCCGAAGTGTACTTTCTCAGCTACCCAGATGTCAGTGCACGCAGAAGTGC
ACCCTTCTCATGACTTAACTGCAGCAGAAATAGGGAAGAGTAAAACCCTCAACTCTGCCTTTGGCTCTGA
ACTGATTTCAGTCAGATTCTAAGCTGTGCTCACTCTTACTCTGAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 73187. Forward Primer - name:073187_F_cDNA_Fem1b, sequence:AAGGGGTAGCTGACTCTGACAC; Reverse Primer - name:073187_N_SP6_cDNA_Fem1b, sequence:TTCAGAGTAAGAGTGAGCACAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17753 same embryo
 EMAGE:17756 same embryo
 EMAGE:17755 same embryo
 EurExpress:euxassay_008916 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS