EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17761

Hey2 hairy/enhancer-of-split related with YRPW motif 2 ( MGI:1341884)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008923. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008923_01	euxassay_008923_02	euxassay_008923_03	euxassay_008923_04

euxassay_008923_05	euxassay_008923_06	euxassay_008923_07	euxassay_008923_08	euxassay_008923_09

euxassay_008923_10	euxassay_008923_11	euxassay_008923_12	euxassay_008923_13	euxassay_008923_14

euxassay_008923_15	euxassay_008923_16	euxassay_008923_17	euxassay_008923_18	euxassay_008923_19

euxassay_008923_20	euxassay_008923_21	euxassay_008923_22	euxassay_008923_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17761_wholemount_strong.wlz

17761_wholemount_moderate.wlz

17761_wholemount_weak.wlz

17761_wholemount_possible.wlz

17761_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17761_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

skin

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

telencephalon ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 16 17 18 19

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 14 15 16

aorta

moderate

regional

moderate expression: see section 09 10 11 12 13

heart ventricle

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

renal cortex

moderate

regional

moderate expression: see section 07 08 09 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35454
Entity Detected:	Hey2, hairy/enhancer-of-split related with YRPW motif 2 ( MGI:1341884)
Sequence:	sense strand is shown >T35454 TGTCTCTCATCTCAGCACCTGTGCCTCCCAGCGGGAGGCAGCAGTGATGACATCCTCCATGGCCCACCAC CATCACCCCTTGCACCCTCACCACTGGGCAGCTGCTTTCCACCATCTCCCCACAGCCCTGCTCCAGCCCA ATGGACTCCACACATCAGAGTCAACCCCATGTCGCCTATCCACATCTTCAGAAGTGCCTTCTGCTCATGG CTCTGCTCTCCTCACAGCAACGTTTGCCCATGCAGATTCTGCTCTTCGGATGCCATCAGGGGGCACCGTT GCACCCTGCGTGCCACCTCTCTCCACCTCTCTTCTGTCTCTTTCGGCCACTGTGCATGCCGCAGCTGCAG CAGCCACTGCAGCTGCACACAGCTTCCCTCTGTCCTTCGCAGGGGCTTTTCCCATGCTCCCGTCCAATGC AGCGGCAGCAGCCGCTGTTGCTGCTGCAACAGCAATCAGCCCACCCTTGTCGGTATCCGCAGCCTCCAGT CCTCAGCAGACAAGCACTGGGACAAACAATAAACCTTACCAACCCTGGGGGACAGAAGTTGGAGCCTTTT AACTTATTCTTGAATCTCTTTGCAATAGTAACTGATGTCGTCCATTTCGGAATCAGCTTCAAACCTCTGC ATCCTGAAGGTAGTCATACAGATATGTACAGTTCCACACAGCAGCAATAAAGCGATTTGAGAAGCAGACT TCACACAAATGGAAATGTAGTATTACTATTTTTTCCCTTTGTTTAAGGCAGCTTGGCAAGTGACATCAGC AACTTGAAAGCAGCACACCAACGTCCATTTAAACTTTCTTGGAAAACACACCAGTGTCACCATCCGGTGG TGCATTAATACTCCTGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99939. Forward Primer - name:099939_F_cDNA_Hey2, sequence:TGTCTCTCATCTCAGCACCTGT; Reverse Primer - name:099939_N_SP6_cDNA_Hey2, sequence:GTCAGGAGTATTAATGCACCACC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17759 same embryo
	EMAGE:17758 same embryo
	EMAGE:17762 same embryo
	EMAGE:17760 same embryo
	EMAGE:17757 same embryo
	EurExpress:euxassay_008923 same experiment
	MGI:4825365 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source