EMAGE mouse gene expression spatial database

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EMAGE:17802

Nrarp Notch-regulated ankyrin repeat protein ( MGI:1914372)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008969. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008969_01	euxassay_008969_02	euxassay_008969_03	euxassay_008969_04

euxassay_008969_05	euxassay_008969_06	euxassay_008969_07	euxassay_008969_08	euxassay_008969_09

euxassay_008969_10	euxassay_008969_11	euxassay_008969_12	euxassay_008969_13	euxassay_008969_14

euxassay_008969_15	euxassay_008969_16	euxassay_008969_17	euxassay_008969_18	euxassay_008969_19

euxassay_008969_20	euxassay_008969_21	euxassay_008969_22	euxassay_008969_23	euxassay_008969_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17802_wholemount_strong.wlz

17802_wholemount_moderate.wlz

17802_wholemount_weak.wlz

17802_wholemount_possible.wlz

17802_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17802_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15 16

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 10 11 21 22 23 24

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 13 14 15 16 17

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 13 14

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 12

pons ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

spinal cord ventricular layer

moderate

regional

moderate expression: see section 13 14 15 16

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 02 03 23

metanephros

moderate

regional

moderate expression: see section 18 19 20 21 22

renal cortex

moderate

regional

moderate expression: see section 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35508
Entity Detected:	Nrarp, Notch-regulated ankyrin repeat protein ( MGI:1914372)
Sequence:	sense strand is shown >T35508 TGGAAATCGGGCTAAGTCTCTACGTCCGCGGCCACGACCAAAGTAGCGCATCCGTGGACCCCAGGGTTCG GCTCTCCGCAAAAGAGCGCCTCCCCTTGGCAGTCCTCTGAGCCCCGCGGGGCCCCAGAGCCTTCCCACGG CCCCGACCGCCTAGAGGGTGGGGGCGGGGCGCAGGCTCCAGTCCGTTTTGAAATTTGAGTCTCACACCGG GAGACTTCGGAATCCCGAGATACCGGATCCTCCGCTTGAAATGTTTTCTCCGGAAGGTGAAAGGCGCGGG CGGACTCGCGCTCCAGCCGCCGCTCGTGAGACCCGGCCGCTCACAGTGCGGCGCCCGCCGCTGGCAGCCG CCAAACAACCATTACCGGGCTCCACCATCTGCGCGCCCTCGCACTTAGGAAGGGAAGGGGACGCTCCGGG TTCCTGATGTCCTCAACTATTTATCACGTGTGTGTATATTTGTGGGTGAAGTTTGTGCGCCTGAGTTTTT TGTTTGGAAATATTGTGCGTGGTTATGGGAGAAAGATGCATTTTTTTTCCTTTAAAACTAAAAACTTGAG TCTACCATTTTTTGGTTGCACTGAAAATACCGCCTAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64815. Forward Primer - name:064815_F_cDNA_Nrarp, sequence:TGGAAATCGGGCTAAGTCTCTA; Reverse Primer - name:064815_N_SP6_cDNA_Nrarp, sequence:GCTAGGCGGTATTTTCAGTGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17801 same embryo
	EurExpress:euxassay_008969 same experiment
	MGI:4826789 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source