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EMAGE:17821

Ckap4 cytoskeleton-associated protein 4 ( MGI:2444926)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17821 EMAGE:17821 EMAGE:17821 EMAGE:17821 EMAGE:17821
"Pseudo-wholemount" of euxassay_009017. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009017_01 euxassay_009017_02 euxassay_009017_03 euxassay_009017_04
EMAGE:17821 EMAGE:17821 EMAGE:17821 EMAGE:17821 EMAGE:17821
euxassay_009017_05 euxassay_009017_06 euxassay_009017_07 euxassay_009017_08 euxassay_009017_09
EMAGE:17821 EMAGE:17821 EMAGE:17821 EMAGE:17821 EMAGE:17821
euxassay_009017_10 euxassay_009017_11 euxassay_009017_12 euxassay_009017_13 euxassay_009017_14
EMAGE:17821 EMAGE:17821 EMAGE:17821
euxassay_009017_15 euxassay_009017_16 euxassay_009017_17

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17821Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17821_wholemount_strong.wlz
17821_wholemount_moderate.wlz
17821_wholemount_weak.wlz
17821_wholemount_possible.wlz
17821_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17821_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36159
Entity Detected:Ckap4, cytoskeleton-associated protein 4 ( MGI:2444926)
Sequence:sense strand is shown

>T36159
GGAGACTGGAGGAAGAGCTACAGCAGCTGAAGGTCGGGGCCCATGGGTCAGAGGAAGGTGCCGTCTTCAA
AGACTCCAAAGCCTTAGAGGAACTGCAGAGGCAGATTGAGGGCCTGGGTGCCAGGCTCCAGTATGTAGAG
GACGGGGTCTACTCAATGCAGGTGGCTTCTGCACGCCACACCGAAAGCCTGGAGTCGCTCCTGTCCAAGA
GCCAGGAGTACGAGCAACGCCTGGCTATGCTGCAGGAGCACGTGGGAAACCTGGGCTCCTCATCTGACCT
GGCCAGCACAGTCAGGAGCCTGGGGGAGACCCAGCTGGCGCTGTCCAGCGACTTGAAGGAGCTGAAGCAG
AGCCTGGGTGAGCTGCCCGGTACCGTGGAGTCACTGCAGGAGCAAGTGCTCTCACTGCTCAGCCAGGACC
AAGCCCAGGCCGAGGGCTTGCCTCCTCAGGACTTCCTGGACAGACTGTCCTCTCTAGACAACCTGAAATC
CTCAGTGAGCCAAGTGGAGTCAGACTTGAAAATGCTCAGGACTGCCGTGGACAGTTTGGTGGCCTATTCC
GTCAAAATCGAAACGAATGAGAATAACTTAGAGTCAGCCAAGGGCTTGCTGGATGACCTGCGGAACGACC
TGGATAGGTTGTTTCTGAAAGTTGAGAAGATCCATGAAAAGATCTAAGTGAACTGCGTGTGTGGGCACAG
TTTCTTACGACCAAAACCAAACAAACAAAAAAAACAAAACAAAAAAAATCCCATTGTTTTCTCCCAGGTG
CCCTCTCATCACCTTCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 82991. Forward Primer - name:082991_F_cDNA_Ckap4, sequence:GGAGACTGGAGGAAGAGCTACA; Reverse Primer - name:082991_N_SP6_cDNA_Ckap4, sequence:GAGAAGGTGATGAGAGGGCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17819 same embryo
 EMAGE:17818 same embryo
 EMAGE:17822 same embryo
 EMAGE:17820 same embryo
 EurExpress:euxassay_009017 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS