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EMAGE:17877

Htt huntingtin ( MGI:96067)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17877 EMAGE:17877 EMAGE:17877 EMAGE:17877 EMAGE:17877
"Pseudo-wholemount" of euxassay_009080. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009080_01 euxassay_009080_02 euxassay_009080_03 euxassay_009080_04
EMAGE:17877 EMAGE:17877 EMAGE:17877 EMAGE:17877 EMAGE:17877
euxassay_009080_05 euxassay_009080_06 euxassay_009080_07 euxassay_009080_08 euxassay_009080_09
EMAGE:17877 EMAGE:17877 EMAGE:17877 EMAGE:17877 EMAGE:17877
euxassay_009080_10 euxassay_009080_11 euxassay_009080_12 euxassay_009080_13 euxassay_009080_14
EMAGE:17877 EMAGE:17877 EMAGE:17877 EMAGE:17877 EMAGE:17877
euxassay_009080_15 euxassay_009080_16 euxassay_009080_17 euxassay_009080_18 euxassay_009080_19
EMAGE:17877 EMAGE:17877 EMAGE:17877
euxassay_009080_20 euxassay_009080_21 euxassay_009080_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17877Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17877_wholemount_strong.wlz
17877_wholemount_moderate.wlz
17877_wholemount_weak.wlz
17877_wholemount_possible.wlz
17877_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17877_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36467
Entity Detected:Htt, huntingtin ( MGI:96067)
Sequence:sense strand is shown

>T36467
CACCCTTCAGAAGATGAGATCCTCATTCAGTACCTGGTGCCTGCCACCTGTAAGGCAGCTGCTGTCCTTG
GAATGGACAAAACTGTGGCAGAGCCAGTCAGCCGCCTACTGGAGAGCACACTGAGGAGCAGCCACCTGCC
CAGCCAGATCGGAGCCCTGCACGGCATCCTCTATGTGTTGGAGTGTGACCTCTTGGATGACACTGCAAAG
CAGCTCATTCCAGTTGTTAGTGACTATCTGCTGTCCAACCTCAAAGGAATAGCCCACTGCGTGAACATTC
ACAGCCAGCAGCATGTGCTGGTAATGTGTGCCACTGCTTTCTACCTGATGGAAAACTACCCTCTGGATGT
GGGACCAGAATTTTCAGCATCTGTGATACAGATGTGTGGAGTAATGCTGTCTGGAAGTGAGGAGTCCACC
CCCTCCATCATTTACCACTGTGCCCTCCGGGGTCTGGAGCGGCTCCTGCTGTCTGAGCAGCTATCTCGGC
TAGACACAGAGTCCTTGGTCAAGCTAAGTGTGGACAGAGTGAATGTACAAAGCCCACACAGGGCCATGGC
AGCCCTAGGCCTGATGCTCACCTGCATGTACACAGGAAAGGAAAAAGCCAGTCCAGGCAGAGCTTCTGAC
CCCAGCCCTGCTACACCTGACAGCGAGTCTGTGATTGTAGCTATGGAGCGAGTGTCTGTTCTCTTTGATA
GGATCCGCAAGGGATTTCCCTGTGAAGCCAGGGTTGTGGCAAGGATCCTGCCTCAGTTCCTAGATGACTT
CT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101124. Forward Primer - name:101124_F_cDNA_Hdh, sequence:CACCCTTCAGAAGATGAGATCC; Reverse Primer - name:101124_N_SP6_cDNA_Hdh, sequence:AGAAGTCATCTAGGAACTGAGGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17878 same embryo
 EMAGE:17874 same embryo
 EMAGE:17876 same embryo
 EMAGE:17875 same embryo
 EMAGE:17873 same embryo
 EurExpress:euxassay_009080 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS