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EMAGE:17892

Il12a interleukin 12a ( MGI:96539)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17892 EMAGE:17892 EMAGE:17892 EMAGE:17892 EMAGE:17892
"Pseudo-wholemount" of euxassay_009594. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009594_01 euxassay_009594_02 euxassay_009594_03 euxassay_009594_04
EMAGE:17892 EMAGE:17892 EMAGE:17892 EMAGE:17892 EMAGE:17892
euxassay_009594_05 euxassay_009594_06 euxassay_009594_07 euxassay_009594_08 euxassay_009594_09
EMAGE:17892 EMAGE:17892 EMAGE:17892 EMAGE:17892 EMAGE:17892
euxassay_009594_10 euxassay_009594_11 euxassay_009594_12 euxassay_009594_13 euxassay_009594_14
EMAGE:17892 EMAGE:17892 EMAGE:17892 EMAGE:17892 EMAGE:17892
euxassay_009594_15 euxassay_009594_16 euxassay_009594_17 euxassay_009594_18 euxassay_009594_19
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euxassay_009594_20 euxassay_009594_21 euxassay_009594_22 euxassay_009594_23 euxassay_009594_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17892Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17892_wholemount_strong.wlz
17892_wholemount_moderate.wlz
17892_wholemount_weak.wlz
17892_wholemount_possible.wlz
17892_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17892_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36497
Entity Detected:Il12a, interleukin 12a ( MGI:96539)
Sequence:sense strand is shown

>T36497
CTCCTAAACCACCTCAGTTTGGCCAGGGTCATTCCAGTCTCTGGACCTGCCAGGTGTCTTAGCCAGTCCC
GAAACCTGCTGAAGACCACAGATGACATGGTGAAGACGGCCAGAGAAAAACTGAAACATTATTCCTGCAC
TGCTGAAGACATCGATCATGAAGACATCACACGGGACCAAACCAGCACATTGAAGACCTGTTTACCACTG
GAACTACACAAGAACGAGAGTTGCCTGGCTACTAGAGAGACTTCTTCCACAACAAGAGGGAGCTGCCTGC
CCCCACAGAAGACGTCTTTGATGATGACCCTGTGCCTTGGTAGCATCTATGAGGACTTGAAGATGTACCA
GACAGAGTTCCAGGCCATCAACGCAGCACTTCAGAATCACAACCATCAGCAGATCATTCTAGACAAGGGC
ATGCTGGTGGCCATCGATGAGCTGATGCAGTCTCTGAATCATAATGGCGAGACTCTGCGCCAGAAACCTC
CTGTGGGAGAAGCAGACCCTTACAGAGTGAAAATGAAGCTCTGCATCCTGCTTCACGCCTTCAGCACCCG
CGTCGTGACCATCAACAGGGTGATGGGCTATCTGAGCTCCGCCTGAAAGGCTCAAGGCCCTCTGCCACAG
CGCCCTCCTCACACAGATAGGAAACAAAGAAAGATTCATAAGAGTCAGGTGGTCTTGGCCTGGTGGGCCT
TAAGCTCCTTCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89581. Forward Primer - name:089581_F_cDNA_Il12a, sequence:CTCCTAAACCACCTCAGTTTGG; Reverse Primer - name:089581_N_SP6_cDNA_Il12a, sequence:CTGAAGGAGCTTAAGGCCCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17896 same embryo
 EMAGE:17895 same embryo
 EMAGE:17891 same embryo
 EMAGE:17894 same embryo
 EMAGE:17893 same embryo
 EurExpress:euxassay_009594 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS