EMAGE mouse gene expression spatial database

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EMAGE:17893

Il12rb2 interleukin 12 receptor, beta 2 ( MGI:1270861)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009595. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009595_01	euxassay_009595_02	euxassay_009595_03	euxassay_009595_04

euxassay_009595_05	euxassay_009595_06	euxassay_009595_07	euxassay_009595_08	euxassay_009595_09

euxassay_009595_10	euxassay_009595_11	euxassay_009595_12	euxassay_009595_13	euxassay_009595_14

euxassay_009595_15	euxassay_009595_16	euxassay_009595_18	euxassay_009595_19	euxassay_009595_20

euxassay_009595_21	euxassay_009595_22	euxassay_009595_23	euxassay_009595_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17893_wholemount_strong.wlz

17893_wholemount_moderate.wlz

17893_wholemount_weak.wlz

17893_wholemount_possible.wlz

17893_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17893_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

strong

regional

strong expression: see section 08 13 14

telencephalon ventricular layer

strong

regional

strong expression: see section 10 11 12

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

midbrain ventricular layer

strong

regional

strong expression: see section 09

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 15 16 moderate expression: see section 05 06 07 08 09 12 13 14

lower jaw incisor

strong

regional

strong expression: see section 13 moderate expression: see section 09 10 14

upper jaw incisor

strong

regional

strong expression: see section 13 moderate expression: see section 09 14

renal cortex

moderate

regional

moderate expression: see section 06 07 08 09 14 15 16 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36499
Entity Detected:	Il12rb2, interleukin 12 receptor, beta 2 ( MGI:1270861)
Sequence:	sense strand is shown >T36499 TTCTGCACCCACTCACATTAACATAGTGGACCTATGTGGCACTGGGTTGCTGGCTCCTCACCAGGTCTCT GCAAAGTCGGAGAACATGGACAACATTCTAGTGACCTGGCAGCCTCCTAAGAAAGCTGATTCTGCTGTTC GGGAGTACATAGTGGAATGGAGAGCTCTCCAACCAGGGAGCATCACGAAGTTTCCCCCACACTGGCTGCG GATCCCCCCGGACAACATGTCTGCTCTGATTTCAGAGAACATAAAGCCCTATATCTGTTATGAAATCAGG GTGCATGCACTGTCAGAGAGCCAAGGAGGGTGCAGCTCCATCCGGGGTGACTCCAAGCACAAAGCACCAG TGAGTGGCCCTCACATTACTGCCATCACAGAGAAAAAGGAACGCCTTTTCATTTCCTGGACCCACATTCC ATTCCCGGAGCAAAGGGGCTGCATCCTCCATTACAGAATATACTGGAAAGAACGAGACTCGACAGCACAA CCTGAGCTCTGCGAAATTCAGTACCGACGCTCTCAAAACTCACATCCAATAAGCAGCCTACAGCCCAGGG TGACATATGTCCTATGGATGACAGCTGTGACAGCTGCTGGTGAAAGTCCCCAAGGAAATGAAAGGGAATT TTGTCCACAGGGCAAAGCCAACTGGAAAGCATTCGTGATATCAAGCATTTGCATCGCTATCATCACG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97914. Forward Primer - name:097914_F_cDNA_Il12rb2, sequence:TTCTGCACCCACTCACATTAAC; Reverse Primer - name:097914_N_SP6_cDNA_Il12rb2, sequence:CGTGATGATAGCGATGCAAAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17896 same embryo
	EMAGE:17895 same embryo
	EMAGE:17891 same embryo
	EMAGE:17894 same embryo
	EMAGE:17892 same embryo
	EurExpress:euxassay_009595 same experiment
	MGI:4825561 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source