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EMAGE:1791

Atf5 activating transcription factor 5 ( MGI:2141857)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:1791
0785d2.jpg This embryo was treated with ProteinaseK for either 3min or 30min.

Expression pattern clarity: no stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
central nervous system
possible possible
Authors stated that in some instances they could not determine expression levels with certainty.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3506442
Entity Detected:Atf5, activating transcription factor 5 ( MGI:2141857)
Sequence:sense strand is shown

>MGI:3506442
AGTCAGCTGCTCTCAGGTACCGCCAGAGGAAGCGGGCAGAGGGCGAGGCCCTGGAGGGCGAGTGCCAGGG
GCTGGAGGCCCGGAACCGGGAGCTGAGGGAGAGGGCTGAGTCAGTGGAGCGGGAGATCCAGTACGTGAAG
GACCTGCTAATTGAGGTGTATAAGGCCCGAAGCCAGAGGACCCGCAGCACCTAGGGTACAGGTCCAGGCT
GGAGAGGGAGGTCTCGTGTACGTCTGTCTCCAGCGTCACACTACCCCTCCATTCCACTTTCCTATCCAGT
CCATCTCTAGGCTTCCCCTCTGCCTAACCCACTGTGGGCTATTTGGCAGAGTCAGTTTCTGTACCCCTTC
AGTGCAACTGAGAAAGCAAGGCCCTCAGGGAACTGGAGGAGGAGCCAGCCTGATCCCTGGCCTCTGGAGT
CTTAAAGCAAGTGTGTTTTTGCAGGTAGTCCTAGTTGGGTGTAGGGGGAGGCTGCCAGGCCTGGCATGAA
CATAAAGCAGGAAGCCACTCTGGGTGATGGC
nt 62 - nt 582 of AB012276.1
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: GTCAGCTGCTCTCAGGTACCG 3'primer: CCATCACCCAGAGTGGCTTCC
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3509135 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE