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EMAGE:17927

Tacstd2 tumor-associated calcium signal transducer 2 ( MGI:1861606)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17927 EMAGE:17927 EMAGE:17927 EMAGE:17927 EMAGE:17927
"Pseudo-wholemount" of euxassay_011694. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011694_01 euxassay_011694_02 euxassay_011694_03 euxassay_011694_04
EMAGE:17927 EMAGE:17927 EMAGE:17927 EMAGE:17927 EMAGE:17927
euxassay_011694_05 euxassay_011694_06 euxassay_011694_07 euxassay_011694_08 euxassay_011694_09
EMAGE:17927 EMAGE:17927 EMAGE:17927 EMAGE:17927 EMAGE:17927
euxassay_011694_10 euxassay_011694_11 euxassay_011694_12 euxassay_011694_13 euxassay_011694_14
EMAGE:17927 EMAGE:17927 EMAGE:17927 EMAGE:17927 EMAGE:17927
euxassay_011694_15 euxassay_011694_16 euxassay_011694_17 euxassay_011694_18 euxassay_011694_19
EMAGE:17927 EMAGE:17927 EMAGE:17927 EMAGE:17927
euxassay_011694_20 euxassay_011694_21 euxassay_011694_22 euxassay_011694_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17927Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17927_wholemount_strong.wlz
17927_wholemount_moderate.wlz
17927_wholemount_weak.wlz
17927_wholemount_possible.wlz
17927_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17927_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
epidermis
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
vibrissa
strong strong
regionalstrong expression: see section 06 07 08 moderate expression: see section 21
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 22 23 weak expression: see section 03 04 20 21
cornea epithelium
weak weak
regionalweak expression: see section 03 04 05 22 23
esophagus
moderate moderate
regionalmoderate expression: see section 09
stomach
moderate moderate
regionalmoderate expression: see section 01 03
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 15
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 17
upper jaw incisor
weak weak
regionalweak expression: see section 15
upper jaw molar
weak weak
regionalweak expression: see section 18
bladder
strong strong
regionalExpression in the fundus region.
kidney calyx
strong strong
regionalstrong expression: see section 05 07 moderate expression: see section 08 weak expression: see section 14 16
kidney pelvis
strong strong
regionalstrong expression: see section 06 15
ureter
strong strong
regionalstrong expression: see section 13
male reproductive system
strong strong
regionalstrong expression: see section 13
urethra of male
strong strong
regionalstrong expression: see section 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37111
Entity Detected:Tacstd2, tumor-associated calcium signal transducer 2 ( MGI:1861606)
Sequence:sense strand is shown

>T37111
CCCACCATTCAGATAGAGCTTCGGCAGAACGCGTCGCAGAAGGGCTTGAGAGACGTGGACATCGCTGATG
CCGCCTACTACTTCGAAAGGGACATTAAAGGCGAGTCACTGTTCATGGGCCGCCGCGGCCTGGACGTGCA
GGTGCGTGGGGAACCCCTGCATGTGGAGCGGACGCTCATCTACTACCTGGACGAGAAGCCCCCCCAGTTC
TCCATGAAGCGCCTCACCGCCGGCGTCATTGCCGTCATCGCTGTCGTCTCGGTAGCGGTAGTGGCTGGTG
TGGTGGTCTTGGTGGTCACCAAACGGAGGAAGTCGGGCAAATACAAAAAGGTGGAGCTTAAGGAGCTGGG
GGAGATGAGAAGCGAACCTAGCTTGTAGGTTTCCTGTAGGATGCCCCGACTTCCTCGGCACCTCAGACCA
GATGGGTTGGCCTGTTAATTCTCAGTCAGGAGGTAATTTTCCTCCCAGCTTTGACCTCTTCCTTCTCTCT
CCACAAACAAGTTCTCTAGATGCTGAGCTCAGGTCACCTCCCTCGCCCTTGGTTTTTTAAGGACGCATTT
CTGAAATTCCTTGTCTAGTCACTGGAAACCTGACCGGGAGAGTTAAGCAAAGGCTAGATAGCTTGGTTAA
GAAGCTAGTGCCAGGCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94849. Forward Primer - name:094849_F_cDNA_Tacstd2, sequence:CCCACCATTCAGATAGAGCTTC; Reverse Primer - name:094849_N_SP6_cDNA_Tacstd2, sequence:CTGCCTGGCACTAGCTTCTTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17926 same embryo
 EMAGE:17924 same embryo
 EMAGE:17925 same embryo
 EMAGE:17928 same embryo
 EurExpress:euxassay_011694 same experiment
 MGI:4828584 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS