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EMAGE:17939

Adad1 adenosine deaminase domain containing 1 (testis specific) ( MGI:103258)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17939 EMAGE:17939 EMAGE:17939 EMAGE:17939 EMAGE:17939
"Pseudo-wholemount" of euxassay_011700. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011700_01 euxassay_011700_02 euxassay_011700_03 euxassay_011700_04
EMAGE:17939 EMAGE:17939 EMAGE:17939 EMAGE:17939 EMAGE:17939
euxassay_011700_05 euxassay_011700_06 euxassay_011700_07 euxassay_011700_08 euxassay_011700_09
EMAGE:17939 EMAGE:17939 EMAGE:17939 EMAGE:17939 EMAGE:17939
euxassay_011700_10 euxassay_011700_11 euxassay_011700_12 euxassay_011700_13 euxassay_011700_14
EMAGE:17939 EMAGE:17939 EMAGE:17939 EMAGE:17939 EMAGE:17939
euxassay_011700_15 euxassay_011700_16 euxassay_011700_17 euxassay_011700_18 euxassay_011700_19
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euxassay_011700_20 euxassay_011700_21 euxassay_011700_22 euxassay_011700_23 euxassay_011700_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17939Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17939_wholemount_strong.wlz
17939_wholemount_moderate.wlz
17939_wholemount_weak.wlz
17939_wholemount_possible.wlz
17939_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17939_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
cochlea
strong strong
regionalstrong expression: see section 16 17 18 moderate expression: see section 19
utricle
strong strong
regionalstrong expression: see section 17 18 moderate expression: see section 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37122
Entity Detected:Adad1, adenosine deaminase domain containing 1 (testis specific) ( MGI:103258)
Sequence:sense strand is shown

>T37122
TTGACGAGCTTCTACAACTGGATGAGCCTGAACCGAGAGTTTTAGAACCAGCAGGACCTCCTCCTATCCC
TGCAGAACCTGTTGTTACACCTGAAGCAGCATATGTTTCAAAAGTGCAATATGAAGGGAGACAAGTCCAG
TATGCCAAGATTTCACAGCTTCTTAAAGAAACATTTGGTCAACTAATTTCTAATCACTCACAATATCTGA
AATGTAGCAGTTCATTGGCTGCTTTCATTATTGAAAGAGCTGGACATCATGAAGTTGTGGCTATAGGCAC
AGGCGAATACAACTACAGCCAGTGCATCAAGCCAAATGGAAGGGTGCTGCACGATACTCATGCAGTTGTT
ACAGCAAGAAGGTCTCTCCTTAGGTACTTTTATAGACAGCTTCTGCTCTTCTATAGCAAAAATCCTGCTA
TGATGGAAAAGTCAATCTTTTGTACAGAACCAGCTTCTAATCTACTTACTCTGAAACAAAACTACAACCT
TTACCTCTATATGAACCAGTTGCCGAAAGGATCAGCCCAGATTAAGTCACAGTTACGCCTTAATCCACAT
TCTATTTCTGCGTTTGAAGCCAATGAAGAGCTGAGTCTCCATGTGGCTGTTGAAGGCAAAATT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 88431. Forward Primer - name:088431_F_cDNA_Tenr, sequence:TTGACGAGCTTCTACAACTGGA; Reverse Primer - name:088431_N_SP6_cDNA_Tenr, sequence:AATTTTGCCTTCAACAGCCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17941 same embryo
 EMAGE:17940 same embryo
 EurExpress:euxassay_011700 same experiment
 MGI:4822970 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS