EMAGE mouse gene expression spatial database

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EMAGE:17962

Galntl1 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 1 ( MGI:1917754)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011719. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011719_01	euxassay_011719_02	euxassay_011719_03	euxassay_011719_04

euxassay_011719_05	euxassay_011719_06	euxassay_011719_07	euxassay_011719_08	euxassay_011719_09

euxassay_011719_10	euxassay_011719_11	euxassay_011719_12	euxassay_011719_13	euxassay_011719_14

euxassay_011719_15	euxassay_011719_16	euxassay_011719_17	euxassay_011719_18	euxassay_011719_19

euxassay_011719_20	euxassay_011719_21	euxassay_011719_22	euxassay_011719_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17962_wholemount_strong.wlz

17962_wholemount_moderate.wlz

17962_wholemount_weak.wlz

17962_wholemount_possible.wlz

17962_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17962_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 08 09 10

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 11 12 13 14 15 16 17 18

medulla oblongata floor plate

moderate

regional

moderate expression: see section 10 11

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 09

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 05 06 15

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 09

metencephalon floor plate

moderate

regional

moderate expression: see section 10 11

pons mantle layer

moderate

regional

moderate expression: see section 05 15

pons ventricular layer

moderate

regional

moderate expression: see section 08 09 12 13

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

ventral grey horn

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37716
Entity Detected:	Galntl1, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 1 ( MGI:1917754)
Sequence:	sense strand is shown >T37716 AGAACGTGGATAGAGAAGCAGCCAACTGTGAAGTGCCAGAAAACCAAGGTCTCTTTTCAGGACATCATCA CCCTGTTGCTGCTTTGCCAGCCCCAGGTCCCAAGCCCAACATATGTCTGCAGCCCCTAGGTGACCACAGT CTTAGCAAGAAACTGGAGAGGGATCTGGGGTATAATTTATTAAGTAGAATAATTTCCACTTCCGCTTAGC CATATGTCACGCCAAGACCTCACACTTGGGCGTGTCCCCACTAACTGGTCATACACCCTGAAATACCCTT ATCTTTGCTAACTGTTTTGGACTCCTACTTTTTCAGGTTCTTTGCCTCTGTAAACATTGCATTGTGGGGC AAGGTGGCTAGTCAGAATTGGACCCCTTTCCTGGAGTATAACCATTCCTAGTCAGTAGAGACTGAGTCAC CCAGGTCAAAACACCGCGCTAAATCCCTTCACCAAAGGCCAGTGGTTGTAGTGGGTTTCGAAGCTGGCTA GTGGCTCCTGTCTGTGTACAGAATAACCTGCTCTCTCCTCTCCCGATACTGAGAACACGGAGACTATAGG TTGGTATCATCCTGGAATCCCGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 83771. Forward Primer - name:083771_F_cDNA_Galntl1, sequence:AGAACGTGGATAGAGAAGCAGC; Reverse Primer - name:083771_N_SP6_cDNA_Galntl1, sequence:ACGGGATTCCAGGATGATACC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17964 same embryo
	EMAGE:17961 same embryo
	EMAGE:17963 same embryo
	EurExpress:euxassay_011719 same experiment
	MGI:4824987 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source