EMAGE mouse gene expression spatial database

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EMAGE:17963

Mamld1 mastermind-like domain containing 1 ( MGI:3045303)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011718. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011718_01	euxassay_011718_02	euxassay_011718_03	euxassay_011718_04

euxassay_011718_05	euxassay_011718_06	euxassay_011718_07	euxassay_011718_08	euxassay_011718_09

euxassay_011718_10	euxassay_011718_11	euxassay_011718_12	euxassay_011718_13	euxassay_011718_14

euxassay_011718_15	euxassay_011718_16	euxassay_011718_17	euxassay_011718_18	euxassay_011718_19

euxassay_011718_20	euxassay_011718_21	euxassay_011718_22	euxassay_011718_23	euxassay_011718_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17963_wholemount_strong.wlz

17963_wholemount_moderate.wlz

17963_wholemount_weak.wlz

17963_wholemount_possible.wlz

17963_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17963_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vertebral axis musculature

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

homogeneous

strong expression: see section 02 03 04 19

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 06 17 18

trigeminal v ganglion

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 15 16 17 18 19 20

vagus x ganglion

strong

homogeneous

strong expression: see section 07 16

dorsal root ganglion

strong

homogeneous

strong expression: see section 07 08 09 16 17

lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37715
Entity Detected:	Mamld1, mastermind-like domain containing 1 ( MGI:3045303)
Sequence:	sense strand is shown >T37715 GGGTCTTTCTCCACCTTACCTTCCAGTGCCATCTCCTCACCCACCACCACCACAACCACCACCACCACCA TTCAGCCCACAGAACTTCACAGCATCCTGCATGTCATCCAGTAGCTTATCTGGCAGTGCTGTGCAAAGCT CGCCCAATGCCTTACTTTCCAGCATGGCCCCCAGCAGCAATGCTTCCCTTGGGCCCACCTTGCCCTATGT TCCAGCAAAGCTCCCGGGCTTGCCTCTCAATCAGCAACCACAATTCAGCCCCCAGAGCTCCATTCTTGCC AATCTGGTGTCCTCTAGTGTCAAAAGCCCTCAAGGACACCTGATATCTGCTCTGCCTACCAGCACCCCAG GGCCATCCCCACCATACCGCCCTGAGAATCTCTCCAGCCCCGGCTTGCCACAGCAGTCCTTCACTCCACA ATACTCTCTGATCAGGAGCCTCACTCCCACCAGCAATCTGCTAAGCCAGCAGCAGCAACAACAGCAACAG CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCATCAGGCAAATTCCATCTTTA AGCCTATGACCAGCAGCCAGCAACCTAAAACCTTGAGCATGATCATGCAGCAGGGCCTATCAAGTTCCAG TCCAGAAGCACCTGAGCCCTTTACCTTCAGCAACACCAAGCCCTTGTCACATTTTGTTTCTGAGCCAAGC CCCCAGAAGATGGCCTCCATGTCTACCCACTCCAGGCAGTCATCTTTGCTCCACTACCTGCCTCAGGCAA CACCAGCACATGCTCCTTCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 86556. Forward Primer - name:086556_F_cDNA_G630014P10Rik, sequence:GGGTCTTTCTCCACCTTACCTT; Reverse Primer - name:086556_N_SP6_cDNA_G630014P10Rik, sequence:AGAAGGAGCATGTGCTGGTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17964 same embryo
	EMAGE:17961 same embryo
	EMAGE:17962 same embryo
	EurExpress:euxassay_011718 same experiment
	MGI:4826071 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source