EMAGE mouse gene expression spatial database

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EMAGE:17971

Gnb2l1 guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1 ( MGI:101849)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011730. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011730_01	euxassay_011730_02	euxassay_011730_03	euxassay_011730_04

euxassay_011730_05	euxassay_011730_06	euxassay_011730_07	euxassay_011730_08	euxassay_011730_09

euxassay_011730_10	euxassay_011730_11	euxassay_011730_12	euxassay_011730_13	euxassay_011730_14

euxassay_011730_15	euxassay_011730_16	euxassay_011730_17	euxassay_011730_18	euxassay_011730_19

euxassay_011730_20	euxassay_011730_21	euxassay_011730_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17971_wholemount_strong.wlz

17971_wholemount_moderate.wlz

17971_wholemount_weak.wlz

17971_wholemount_possible.wlz

17971_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17971_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37726
Entity Detected:	Gnb2l1, guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1 ( MGI:101849)
Sequence:	sense strand is shown >T37726 TCCCGAGACAAGACCATAAAGTTATGGAATACTCTGGGTGTCTGCAAGTACACGGTCCAGGATGAGAGTC ATTCAGAATGGGTGTCTTGTGTCCGCTTCTCCCCGAACAGCAGCAACCCTATCATCGTCTCCTGCGGATG GGACAAGCTGGTCAAGGTGTGGAATCTGGCTAACTGCAAGCTAAAGACCAACCACATTGGCCACACTGGC TACCTGAACACAGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCTA TGCTGTGGGATCTCAATGAAGGCAAGCACCTCTACACTTTAGATGGTGGGGACATCATCAATGCCTTGTG CTTCAGCCCCAACCGCTACTGGCTCTGCGCTGCCACTGGCCCCAGCATCAAGATCTGGGACTTGGAGGGC AAGATCATTGTAGATGAATTGAAGCAAGAAGTTATCAGCACCAGCAGCAAGGCAGAGCCACCCCAGTGTA CCTCTTTGGCATGGTCTGCTGATGGCCAGACTCTGTTTGCTGGCTATACAGACAACTTGGTGCGAGTATG GCAGGTAACTATTGGTACCCG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95159. Forward Primer - name:095159_F_cDNA_Gnb2-rs1, sequence:TCCCGAGACAAGACCATAAAGT; Reverse Primer - name:095159_N_SP6_cDNA_Gnb2-rs1, sequence:CGGGTACCAATAGTTACCTGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17970 same embryo
	EMAGE:17972 same embryo
	EMAGE:17969 same embryo
	EurExpress:euxassay_011730 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source