EMAGE mouse gene expression spatial database

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EMAGE:17986

Dnali1 dynein, axonemal, light intermediate polypeptide 1 ( MGI:1922813)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011747. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011747_01	euxassay_011747_02	euxassay_011747_03	euxassay_011747_04

euxassay_011747_05	euxassay_011747_06	euxassay_011747_07	euxassay_011747_08	euxassay_011747_09

euxassay_011747_10	euxassay_011747_11	euxassay_011747_12	euxassay_011747_13	euxassay_011747_14

euxassay_011747_15	euxassay_011747_16	euxassay_011747_17	euxassay_011747_18	euxassay_011747_19

euxassay_011747_20	euxassay_011747_21	euxassay_011747_22	euxassay_011747_23	euxassay_011747_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17986_wholemount_strong.wlz

17986_wholemount_moderate.wlz

17986_wholemount_weak.wlz

17986_wholemount_possible.wlz

17986_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17986_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 05 06 07 08 10 11 14 17 19 20

choroid invagination

weak

regional

weak expression: see section 05 06 07 08 15 16 17 18 19

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 05 06 07 08 10 11 14 17 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16 17

pharynx epithelium

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37680
Entity Detected:	Dnali1, dynein, axonemal, light intermediate polypeptide 1 ( MGI:1922813)
Sequence:	sense strand is shown >T37680 ACTCCCAGTGTTTTGACGAACTGATCCGGGAGGTCACCATCAACTGCGCAGAGAGAGGGCTGCTGTTGCT GCGTGTCCGGGACGAGATCCACATGACCATCGCTGCCTACCAGACTCTGTATGAGAGCAGCGTGGCATTT GGCATGAGGAAGGCACTGCAGGCCGAACAGGGGAAGTCAGACATGGAGAGGAAAATTACAGAATTGGAGA CAGAAAAGAGGGACTTGGAGAGGCAAGTGAATGAACAGAAGGCGAAATGCGAGGCCACCGAGAAGCGGGA GAGTGAGAGGCGACAGGTGGAGGAGAAGAAGCACAACGAGGAGATCCAGTTCCTGAAACGGACCAACCAG CAACTGAAGGCCCAACTGGAAGGCATTATCGCACCGAAGAAGTGATCATTTCCACATGGGTCTCAGCAGG CCCCTTGCCCCATAAGCCCATTGTAATAAAAGCCAGTTTCCAGAGTGAACAAGCCACCCCTTCCCTGGTC ACCACTCATGTCATTGTTACAACTGTTGTCCCAGAGTCATGAAACACCTAAGCCAGGTTCCTGGCTAGGC AATGGAAGGTGGGAGAACCCGGTGTCCATGTGCAGCTTAAATTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68327. Forward Primer - name:068327_F_cDNA_Dnali1, sequence:ACTCCCAGTGTTTTGACGAACT; Reverse Primer - name:068327_N_SP6_cDNA_Dnali1, sequence:CCAATTTAAGCTGCACATGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17985 same embryo
	EMAGE:17987 same embryo
	EMAGE:17988 same embryo
	EurExpress:euxassay_011747 same experiment
	MGI:4824359 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source