EMAGE mouse gene expression spatial database

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EMAGE:17994

Efhc1 EF-hand domain (C-terminal) containing 1 ( MGI:1919127)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011763. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011763_01	euxassay_011763_02	euxassay_011763_03	euxassay_011763_04

euxassay_011763_05	euxassay_011763_06	euxassay_011763_07	euxassay_011763_08	euxassay_011763_09

euxassay_011763_10	euxassay_011763_11	euxassay_011763_12	euxassay_011763_13	euxassay_011763_14

euxassay_011763_15	euxassay_011763_16	euxassay_011763_17	euxassay_011763_18	euxassay_011763_19

euxassay_011763_20	euxassay_011763_21	euxassay_011763_22	euxassay_011763_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17994_wholemount_strong.wlz

17994_wholemount_moderate.wlz

17994_wholemount_weak.wlz

17994_wholemount_possible.wlz

17994_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17994_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon roof plate

weak

regional

weak expression: see section 12 13

choroid invagination

weak

regional

weak expression: see section 05 06 07 08 09 10 11 15 16 17 18

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 11 12 13 16 17 18 19 20 moderate expression: see section 10 14 15

pharynx epithelium

moderate

regional

moderate expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37698
Entity Detected:	Efhc1, EF-hand domain (C-terminal) containing 1 ( MGI:1919127)
Sequence:	sense strand is shown >T37698 CTACCACTGGAAGGACCTCAACCGTGGAATCAACCTTACAGTGTACGGCAAAACCTTCCGAATTGTTGAC TGTGACCGATTCACTCAGGACTTTTTGGAAAGCCAAGGCATTGAATTAAATCCATCAGAGAAGATACCCC TGGATCCTTACACTCAACTCCGGAAAGAACCTGTGCGCAAATATGTCACCCCGTCAGACTTTGACCAACT GAAACAGTTTCTCACCTTTGACAAACAGGTCCTTCGATTCTATGCCATCTGGGACGACACGGATAGCTTG TTCGGGGAATGTCGTCACTACATCATTCATTACTACCTCATGGATGACACCGTGGAGATCAGAGAGGTCC ACGAACGGAACAACGGGAGAGACCCCTTCCCACTCCTCATGAACCGACAGCGTATGCCCAAAGTTTTGGT GGAGAATGCAAAGAATTTCCCGAAGTGTGTGCTGGAAATCTCAGACCAAGAGGTGCTGGAGTGGTACACT GCCAAGGACTTCATTGTGGGGAAGCCGCTCACCATCCTGGGGAGGACCTTCTTCATCTACGACTGTGACC CATTCACACGACAGTTCTACAAAGACAAGTTTGGAATGCCTGACTTACCACCAGTTGATGTGACCAAGAA GGAGCCACCTCCAGTAAAACAGGAGTTGCCTCCTTATAATGGCTATGGACTAATTGAAGACTCTGCTCAG AATTGTTTTGCTCTCATTCCTAAAGCTCCCAGAAAAGATGTCGTTAAAATGCTGATGAATGACAATAAGG TGCTCCGCTACCTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75482. Forward Primer - name:075482_F_cDNA_Efhc1, sequence:CTACCACTGGAAGGACCTCAAC; Reverse Primer - name:075482_N_SP6_cDNA_Efhc1, sequence:CCAGGTAGCGGAGCACCTTAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17995 same embryo
	EMAGE:17997 same embryo
	EMAGE:17996 same embryo
	EMAGE:17993 same embryo
	EurExpress:euxassay_011763 same experiment
	MGI:4824482 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source