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EMAGE:17995

Btaf1 BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, (Mot1 homolog, S. cerevisiae) ( MGI:2147538)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17995 EMAGE:17995 EMAGE:17995 EMAGE:17995 EMAGE:17995
"Pseudo-wholemount" of euxassay_011758. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011758_01 euxassay_011758_02 euxassay_011758_03 euxassay_011758_04
EMAGE:17995 EMAGE:17995 EMAGE:17995 EMAGE:17995 EMAGE:17995
euxassay_011758_05 euxassay_011758_06 euxassay_011758_07 euxassay_011758_08 euxassay_011758_09
EMAGE:17995 EMAGE:17995 EMAGE:17995 EMAGE:17995 EMAGE:17995
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EMAGE:17995 EMAGE:17995 EMAGE:17995 EMAGE:17995 EMAGE:17995
euxassay_011758_15 euxassay_011758_16 euxassay_011758_17 euxassay_011758_18 euxassay_011758_19
EMAGE:17995 EMAGE:17995 EMAGE:17995 EMAGE:17995 EMAGE:17995
euxassay_011758_20 euxassay_011758_21 euxassay_011758_22 euxassay_011758_23 euxassay_011758_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17995Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17995_wholemount_strong.wlz
17995_wholemount_moderate.wlz
17995_wholemount_weak.wlz
17995_wholemount_possible.wlz
17995_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17995_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37697
Entity Detected:Btaf1, BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, (Mot1 homolog, S. cerevisiae) ( MGI:2147538)
Sequence:sense strand is shown

>T37697
CCCTCCATCGATGTTCTTCTACTCACAACTCACGTTGGTGGCCTGGGCCTGAACTTGACTGGAGCTGACA
CTGTGGTGTTTGTGGAGCATGACTGGAACCCTATGCGAGATCTGCAGGCCATGGACCGGGCCCATCGTAT
TGGGCAGAAACGTGTGGTTAATGTCTACCGGTTGATAACCAGAGGAACACTAGAAGAAAAGATAATGGGC
TTGCAGAAATTCAAGATGAACATCGCAAATACTGTTATTAGCCAAGAAAACTCTAGCTTACAGAGTATGG
GCACTGATCAACTTCTTGACCTGTTCACTCTAGATAAGGATGGAAAAGCAGAAAAGGCTGACAGCTCCAC
TTCTGGGAAAGCAAGCATGAAATCAGTTCTTGAGAACCTGAGTGACCTCTGGGACGCCGAGCAGTATGAT
TCGGAGTACAACCTGGAGACCTTCATGCGCTCTCTCGAGTGACTGCTGCGTGCCGTGCCGTGCCCGGTGC
AACTGCTGCTAGCTCAGTTACCTTCTGCTGATATTCAGCAGACTAAGTCTATGCGAACTTTAACTGAATG
TATAAATAGATCTGAAACTACTCAGCTTAGTGCTGGCTCTTGCTTCATTGGGGAACAAGTTATTCTCAAG
TATTTGCACTGTATTAAATACGATGTTAATGTGAAAAGGTTTAAATTTTACATGCTGAATAGCTGCAGAC
AGGAAGCAGCCAGGTTTCCATGTGCTACCAGGAGGTGCTTCTGCAGGGACAGCCTCCTCTACAGTCACTT
TCTCCACCGTGAGATCCACCAGTGCTGTGCTGTTTGTATGCGTTTGTGTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 86111. Forward Primer - name:086111_F_cDNA_E430027O22Rik, sequence:CCCTCCATCGATGTTCTTCTAC; Reverse Primer - name:086111_N_SP6_cDNA_E430027O22Rik, sequence:GAACACAAACGCATACAAACAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17994 same embryo
 EMAGE:17997 same embryo
 EMAGE:17996 same embryo
 EMAGE:17993 same embryo
 EurExpress:euxassay_011758 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS