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EMAGE:180

Epha4 Eph receptor A4 ( MGI:98277)
TS11 (7.75 dpc)
in situ hybridisation

Data Images
EMAGE:180 EMAGE:180
Fig 2B, Nieto et al, 1992 [PMID:1295734] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 2A, Nieto et al, 1992 [PMID:1295734] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Corresponding brightfield and darkfield images are shown below
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regionalA broad domain of expression is seen in the hindbrain. Higher levels of expression are coincident with the domain of Egr2 (Krox20) expression; lower levels extend to a boundary in the posterior hindbrain
embryo mesoderm
detected detected
gradedExpression levels were higher in more anterior, mature regions of trunk mesoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17719
Entity Detected:Epha4, Eph receptor A4 ( MGI:98277)
Sequence:sense strand is shown

>MGI:17719
GAAGACCTATGTGGATCCTCACACTTACGAAGACCCCAACCAGGCTGTACTCAAGTTTACCACCGAGATC
CACCCATCCTGTGTGGCAAGGCAGAAGGTCATTGGAGCAGGAGAGTTTGGAGAGGTCTATAAAGGGACGC
TGAAGGCATCCTCGGGGAAGAAGGAGATACCGGTGGCCATCAAGACACTGAAAGCGGGCTACACTGAGAA
GCAGCGGGTGGACTTCCTGAGCGAGGCCAGCATCATGGGCCAGTTTAGCCACCACAATATCATCCGCCTG
GAGGCGGTGGTCTCTAAATACAAACCCATGATGATTATCACAGAGTACATGGAGAATGGAGCGCTAGACA
AGTTCCTTAGGGAGAAGGATGGTGAGTTCAGCGTACTTCAGTTGGTGGGCATGCTGAGGGGTATCG
nt 1848 - nt 2263 of NM_010139.1
Notes:The probe used in this study by Nieto et al, 1992 [PMID:1295734] is described and depicted in Fig 1a of that paper (as "probe 2")
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:7.75 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Nieto et al, 1992 [PMID:1295734] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1295734] Nieto MA, Gilardi-Hebenstreit P, Charnay P, Wilkinson DG 1992 A receptor protein tyrosine kinase implicated in the segmental patterning of the hindbrain and mesoderm. Development (116):1137-50
Links:MGI:1335751 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI