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EMAGE:18056

Tpi1 triosephosphate isomerase 1 ( MGI:98797)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18056 EMAGE:18056 EMAGE:18056 EMAGE:18056 EMAGE:18056
euxassay_011841_01 euxassay_011841_02 euxassay_011841_03 euxassay_011841_04 euxassay_011841_05
EMAGE:18056 EMAGE:18056 EMAGE:18056 EMAGE:18056 EMAGE:18056
euxassay_011841_06 euxassay_011841_07 euxassay_011841_08 euxassay_011841_09 euxassay_011841_10
EMAGE:18056 EMAGE:18056 EMAGE:18056 EMAGE:18056 EMAGE:18056
euxassay_011841_11 euxassay_011841_12 euxassay_011841_13 euxassay_011841_14 euxassay_011841_15
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euxassay_011841_16 euxassay_011841_17 euxassay_011841_18

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18056Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18056_wholemount_strong.wlz
18056_wholemount_moderate.wlz
18056_wholemount_weak.wlz
18056_wholemount_possible.wlz
18056_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18056_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37173
Entity Detected:Tpi1, triosephosphate isomerase 1 ( MGI:98797)
Sequence:sense strand is shown

>T37173
AAGGAGGAGTTCTGCTTCACAGCCATCTATATAAGTGGCCAGTGGAGAGAGCCGTGCGTTTGTACTGACC
TTCAGAGACTTGAGCCTGGTACAATGGCGCCTACCAGGAAGTTCTTCGTTGGGGGCAACTGGAAGATGAA
CGGGAGGAAGAAGTGCCTGGGAGAACTCATCTGCACCCTGAACGCAGCCAACGTGCCGGCAGGCACCGAG
GTGGTTTGTGCACCGCCCACCGCTTACATCGACTTTGCCAGACAGAAGCTGGATCCCAAAATTGCTGTGG
CCGCACAGAACTGCTACAAAGTGACCAATGGGGCCTTCACTGGGGAAATCAGCCCTGGCATGATCAAAGA
CTTAGGAGCCACCTGGGTCGTGCTGGGGCACTCAGAAAGAAGACATGTCTTTGGAGAATCAGATGAGCTG
ATTGGCCAGAAAGTGAGCCACGCCCTAGCAGAGGGACTCGGGGTGATCGCCTGCATCGGGGAGAAGCTAG
ACGAAAGGGAAGCCGGCATCACCGAGAAGGTCGTGTTCGAGCAAACCAAGGTCATCGCAGATAATGTGAA
AGACTGGAGCAAGGTGGTCCTGGCCTATGAACCTGTGTGGGCCATTGGGACTGGCAAGACGGCAACCCCT
CAGCAGGCACAGGAAGTACACGAGAAGCTCCGGGGATGGCTGAAATCCAATGTCAATGATGGGGTGGCTC
AGAGCACCCGGATCATTTATGGAGGTTCTGTGACTGGAGCAACCTGCAAAGAGCTGGCAAGCCAGCCTGA
CGTGGACGGCTTCCTTGTGGGTGGCGCATCTCTCAAGCCTGAATTTGTGGACATCATCAATGCCAAACAA
TGAGCACTGCCCATCCCTCTACCTGCCTGCATAGCATGGACTAGGTTGCCCAGAAGTTTAGTAACTGCTC
CC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89671. Forward Primer - name:089671_F_cDNA_Tpi1, sequence:AAGGAGGAGTTCTGCTTCACAG; Reverse Primer - name:089671_N_SP6_cDNA_Tpi1, sequence:GGGAGCAGTTACTAAACTTCTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18054 same embryo
 EMAGE:18055 same embryo
 EurExpress:euxassay_011841 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS