EMAGE mouse gene expression spatial database

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EMAGE:18073

E2f7 E2F transcription factor 7 ( MGI:1289147)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011832. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011832_01	euxassay_011832_02	euxassay_011832_03	euxassay_011832_04

euxassay_011832_05	euxassay_011832_06	euxassay_011832_07	euxassay_011832_08	euxassay_011832_09

euxassay_011832_10	euxassay_011832_11	euxassay_011832_12	euxassay_011832_13	euxassay_011832_14

euxassay_011832_15	euxassay_011832_16	euxassay_011832_17	euxassay_011832_18	euxassay_011832_19

euxassay_011832_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18073_wholemount_strong.wlz

18073_wholemount_moderate.wlz

18073_wholemount_weak.wlz

18073_wholemount_possible.wlz

18073_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18073_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 06 07 10 11

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

weak

regional

weak expression: see section 08 09 10 11 12 13

renal cortex

weak

regional

weak expression: see section 06 07 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37692
Entity Detected:	E2f7, E2F transcription factor 7 ( MGI:1289147)
Sequence:	sense strand is shown >T37692 GGAGTTGCAGGAAGGAAAACTAAAGTCTGGGTGCCTTGTGGCTGCTCCTCCAGCAACGATGAACTATGAG GTCACACTGTTTAAAACAAAAAAATCCTCCTGTGCTCCGGTCCGTTCACTCTGTGCCCCCTGTATCCTAG TTTAAATGTTCACAGTAGAGTGCCGGGGTGTGTGCCTTCTCAAGGTCACCGTTTATAATAAGACGAATCG GTACGACAGAGCAGAACGGAGACCGTTGGGACCTGGATAAGCGTTTTGGTTGAATTGTGAAGCACAATGA CAGGGAGAGAGACATAGAGCAATAGACAATTCTTTTATTTTCTTTTTCTCTTACACATTTTCCTGGTGTT CGGAGGCGAAGAGGGAGAAGGGGCACCCTGTCCATTTCCAAGGTGTTCATTTGTACGTTCCGTGGTCCTC CCAGAAGGAGCACGGGGATTGGCTGTTCCAACAAATAGTCCTGGAAAGTGCACGTGGCCCTTCGTGAGCC AGGTTGCTTTAGGGTTCCCTGTGTTCTACACTGATGTTACCGCACACCTGTCTCTTCAGAGTAAATCTTT CCCCCTCTACTTCCTTTTCTCTTCTTCCAGTCTGTCTTCTATTTAAACTTCTTAGAGTTAGAGTCTTAAT ATAAGGCTATTTTTCCTTTGTATTTTTGTACCCTGCGAAATACTATATACAAGGATAAAAAATAATAATT TAAGAGACTCTGGGAGTCTGATCAGAGTAGCTTTATATTAACTACCAGATACTTTTAGCCTTACTACCCC CAACACTAGCTTTGGGAAACTTGGGATAGGTAGCCTGTTTAACACATTCGCTACTTAATCAAATTTTTTG TCAACACTAAGCTTATAAAGTTGGAAAAAAAAAGGTATTGTTCCCTTTTTTAAAAAAAGTTTTGTTTTTT TTTTTCCTGAGTCAGGTAAAAATTGCATGGTGTCTATGACTGAAGCCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96334. Forward Primer - name:096334_F_cDNA_E2f7, sequence:GGAGTTGCAGGAAGGAAAACTA; Reverse Primer - name:096334_N_SP6_cDNA_E2f7, sequence:GTGGCTTCAGTCATAGACACCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18076 same embryo
	EMAGE:18074 same embryo
	EMAGE:18077 same embryo
	EMAGE:18075 same embryo
	EurExpress:euxassay_011832 same experiment
	MGI:4824452 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source