Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18086

1110028C15Rik RIKEN cDNA 1110028C15 gene ( MGI:1915941)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18086 EMAGE:18086 EMAGE:18086 EMAGE:18086 EMAGE:18086
"Pseudo-wholemount" of euxassay_011923. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011923_01 euxassay_011923_02 euxassay_011923_03 euxassay_011923_04
EMAGE:18086 EMAGE:18086 EMAGE:18086 EMAGE:18086 EMAGE:18086
euxassay_011923_05 euxassay_011923_06 euxassay_011923_07 euxassay_011923_08 euxassay_011923_09
EMAGE:18086 EMAGE:18086 EMAGE:18086 EMAGE:18086 EMAGE:18086
euxassay_011923_10 euxassay_011923_11 euxassay_011923_12 euxassay_011923_13 euxassay_011923_14
EMAGE:18086 EMAGE:18086 EMAGE:18086 EMAGE:18086 EMAGE:18086
euxassay_011923_15 euxassay_011923_16 euxassay_011923_17 euxassay_011923_18 euxassay_011923_19
EMAGE:18086 EMAGE:18086 EMAGE:18086
euxassay_011923_20 euxassay_011923_21 euxassay_011923_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18086Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18086_wholemount_strong.wlz
18086_wholemount_moderate.wlz
18086_wholemount_weak.wlz
18086_wholemount_possible.wlz
18086_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18086_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36088
Entity Detected:1110028C15Rik, RIKEN cDNA 1110028C15 gene ( MGI:1915941)
Sequence:sense strand is shown

>T36088
AAGAGGAAAAAGCCTACAACCCTCTCTGCTGGATCCATCCCCACCTCCTCCGGTCAGGGTTCCAGAACAG
GAGGAGGCCCACATGCATCCATCTTTCATGTTCAGGGAACATGCCGATGTCTTTTTTTTCTTCCTGAATC
ACAGAAGTAGCTAGCTTCTTACCTACACAAATGCTAGTAACTTGTGTTTGCCAATAATACAAATCACAAG
CACTAAGATATTAACTGCTGCAATTTGATGACAAATCACACCTCGGGGCATTTGTTGAGGTACGAGCCCC
TCCCTGATAGTACTGTTGATTTGCATCAAATGACAACTATTCTTTGTTTCTATTCTCATGGCAGTTCACT
AAAACTGCCTCAATAATATGAGAATATGCTAGTGTGTATACAGAATATATACACATACAGTGCACACATA
TGTAGTCTTAAAACTGCATGTATGAATTACTCTATGCAATATAAGTTGCCATGAATACCTAGCACAGGTG
CTGACTTATAGCAGACACCTGAAAGCTTTGACTGTGGAGAGGGAGTTGTTTTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70252. Forward Primer - name:070252_F_cDNA_C430010P07Rik, sequence:AAGAGGAAAAAGCCTACAACCC; Reverse Primer - name:070252_N_SP6_cDNA_C430010P07Rik, sequence:AAAAACAACTCCCTCTCCACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18089 same embryo
 EMAGE:18088 same embryo
 EMAGE:18084 same embryo
 EMAGE:18087 same embryo
 EMAGE:18085 same embryo
 EurExpress:euxassay_011923 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS