EMAGE mouse gene expression spatial database

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EMAGE:18105

Cbl Casitas B-lineage lymphoma ( MGI:88279)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011848. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011848_01	euxassay_011848_02	euxassay_011848_03	euxassay_011848_04

euxassay_011848_05	euxassay_011848_06	euxassay_011848_07	euxassay_011848_08	euxassay_011848_09

euxassay_011848_10	euxassay_011848_11	euxassay_011848_12	euxassay_011848_13	euxassay_011848_14

euxassay_011848_15	euxassay_011848_16	euxassay_011848_17	euxassay_011848_18	euxassay_011848_19

euxassay_011848_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18105_wholemount_strong.wlz

18105_wholemount_moderate.wlz

18105_wholemount_weak.wlz

18105_wholemount_possible.wlz

18105_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18105_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36120
Entity Detected:	Cbl, Casitas B-lineage lymphoma ( MGI:88279)
Sequence:	sense strand is shown >T36120 TATTAAGGCAAGGAGCAGAAGGTGCTCCTTCCCCAAATTACGACGATGATGATGATGAACGAGCTGATGA TTCTCTCTTCATGATGAAGGAGTTGGCAGGTGCCAAGGTGGAAAGGCCTTCCTCTCCATTCTCCATGGCC CCACAAGCTTCCCTTCCTCCAGTGCCACCAAGACTTGACCTTCTACAGCAGCGAGCACCTGTTCCTGCCA GCACTTCAGTTCTGGGGACTGCTTCCAAGGCTGCTTCTGGCTCCCTTCATAAAGACAAACCATTGCCAAT ACCTCCCACACTTCGAGATCTTCCACCACCACCCCCTCCAGACCGGCCTTACTCTGTTGGAGCAGAAACA AGGCCTCAGAGACGCCCTCTGCCTTGTACACCAGGCGATTGTCCATCTAGAGACAAACTGCCCCCTGTCC CTTCTAGCCGCCCAGGGGACTCGTGGTTGTCTCGGACCATCCCTAAAGTACCAGTAGCTACTCCAAACCC TGGTGATCCTTGGAATGGGAGAGAATTGACCAATCGGCACTCGCTTCCATTCTCATTGCCCTCACAAATG GAACCCAGAGCAGATGTCCCTAGGCTTGGAAGCACATTTAGTCTGGATACCTCTATGACTATGAATAGCA GCCCAGTAGCAGGTCCAGAGAGTGAGCACCCAAAGATCAAGCCTTCCTCGTCTGCCAACGCCATTTACTC TCTGGCTGCCAGGCCTCTTCCTATGCCAAAACTGCCACCTGGGGAGCAAGGGGAAAGTGAAGAGGACACA GAAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97494. Forward Primer - name:097494_F_cDNA_Cbl, sequence:TATTAAGGCAAGGAGCAGAAGG; Reverse Primer - name:097494_N_SP6_cDNA_Cbl, sequence:ATTCTGTGTCCTCTTCACTTTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18106 same embryo
	EMAGE:18108 same embryo
	EMAGE:18104 same embryo
	EMAGE:18107 same embryo
	EMAGE:18103 same embryo
	EurExpress:euxassay_011848 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source