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EMAGE:18106

Cbln4 cerebellin 4 precursor protein ( MGI:2154433)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18106 EMAGE:18106 EMAGE:18106 EMAGE:18106 EMAGE:18106
"Pseudo-wholemount" of euxassay_011849. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011849_01 euxassay_011849_02 euxassay_011849_03 euxassay_011849_04
EMAGE:18106 EMAGE:18106 EMAGE:18106 EMAGE:18106 EMAGE:18106
euxassay_011849_05 euxassay_011849_06 euxassay_011849_07 euxassay_011849_08 euxassay_011849_09
EMAGE:18106 EMAGE:18106 EMAGE:18106 EMAGE:18106 EMAGE:18106
euxassay_011849_10 euxassay_011849_11 euxassay_011849_12 euxassay_011849_13 euxassay_011849_14
EMAGE:18106 EMAGE:18106 EMAGE:18106 EMAGE:18106 EMAGE:18106
euxassay_011849_15 euxassay_011849_16 euxassay_011849_17 euxassay_011849_18 euxassay_011849_19
EMAGE:18106
euxassay_011849_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18106Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18106_wholemount_strong.wlz
18106_wholemount_moderate.wlz
18106_wholemount_weak.wlz
18106_wholemount_possible.wlz
18106_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18106_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
midbrain mantle layer
moderate moderate
single cellmoderate expression: see section 10 weak expression: see section 08 09 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36121
Entity Detected:Cbln4, cerebellin 4 precursor protein ( MGI:2154433)
Sequence:sense strand is shown

>T36121
TGCTGGTGATAAAGATGTGACCCGTGAAGCCGCCACTAATGGAGTGCTCCTGTACCTGGACAAAGAAGAT
AAGGTCTACCTAAAACTGGAGAAAGGTAACTTGCTCGGTGGCTGGCAGTATTCCACGTTTTCTGGCTTTC
TGGTGTTTCCTCTATAGAACCCGACTTCTCCGTGATGCCCAAGAGTGAGGGCGCCCACCCCGGGTTATCA
GAAGAGCACTCTTGGATTGATGTCTCTTACTGTTTTGTTCATGGCTGACTATGGATTCTCTCTGAGAATT
CTAGACCTGTCTGGACAGACACAAAGTTTCACAGATCATTTTCGTGTGTGTGCCAGTTTCCTTATATGTG
GACTGGGACTCTAAAGCTGAAAAATCCCCGATCTGCTTACATGTTAACTGTCAGAAGCCGTCTCAAGGTT
TGTCCTGTTTAGTTTCCTGCTGTTACAGCATTGGCTGCAGATGTGGGTTTTCTTTATTTCTTTGTTTTTG
AAAGACAGGCTAATGGGTCTGAAAGGTAGAAAACTTGGAAGTTCTGACTTCAGTGGTTATAGTGGAACTG
CCAAAGACATGTATACACGTGTGGATACATTGATTATTAATTATACTTTTTAAACAAATTATAATTACTT
TTGAATTTGTTTGTTTTGTTCTCTTAAGGTAACTGGGATTGTATTTTAGTGACTGGCGTTGTGTTCTCTC
TACCCATAAGGTAACAAATGTCTTGCCTCAAAATGTCCCTTAACTCTGAACTCACCCACATGACCTCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68892. Forward Primer - name:068892_F_cDNA_Cbln4, sequence:TGCTGGTGATAAAGATGTGACC; Reverse Primer - name:068892_N_SP6_cDNA_Cbln4, sequence:GGAGGTCATGTGGGTGAGTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18105 same embryo
 EMAGE:18108 same embryo
 EMAGE:18104 same embryo
 EMAGE:18107 same embryo
 EMAGE:18103 same embryo
 EurExpress:euxassay_011849 same experiment
 MGI:4823655 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS