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EMAGE:18110

Tsga10ip testis specific 10 interacting protein ( MGI:1925556)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18110 EMAGE:18110 EMAGE:18110 EMAGE:18110 EMAGE:18110
"Pseudo-wholemount" of euxassay_011930. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011930_01 euxassay_011930_02 euxassay_011930_03 euxassay_011930_04
EMAGE:18110 EMAGE:18110 EMAGE:18110 EMAGE:18110 EMAGE:18110
euxassay_011930_05 euxassay_011930_06 euxassay_011930_07 euxassay_011930_08 euxassay_011930_09
EMAGE:18110 EMAGE:18110 EMAGE:18110 EMAGE:18110 EMAGE:18110
euxassay_011930_10 euxassay_011930_11 euxassay_011930_12 euxassay_011930_13 euxassay_011930_14
EMAGE:18110 EMAGE:18110 EMAGE:18110 EMAGE:18110 EMAGE:18110
euxassay_011930_15 euxassay_011930_16 euxassay_011930_17 euxassay_011930_18 euxassay_011930_19
EMAGE:18110 EMAGE:18110 EMAGE:18110 EMAGE:18110
euxassay_011930_20 euxassay_011930_21 euxassay_011930_22 euxassay_011930_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18110Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18110_wholemount_strong.wlz
18110_wholemount_moderate.wlz
18110_wholemount_weak.wlz
18110_wholemount_possible.wlz
18110_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18110_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 10 11 12 15 16 17 18 19 20 21 22 23
radius
moderate moderate
regionalmoderate expression: see section 01
humerus
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 21 22 23
fibula
moderate moderate
regionalmoderate expression: see section 01 02 23
tibia
moderate moderate
regionalmoderate expression: see section 01 02 03 23
femur
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 17 18 19 20 21 22
meckel's cartilage
moderate moderate
homogeneousmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
axial skeleton
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17
basioccipital bone
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 17 18 19
basisphenoid bone
moderate moderate
homogeneousmoderate expression: see section 09 10 11 12 13 14 15 16
temporal bone petrous part
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 21 22
vault of skull
moderate moderate
homogeneousmoderate expression: see section 02 03
orbito-sphenoid
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06 07 08 19 20 21 22
scapula
moderate moderate
regionalmoderate expression: see section 03 04 05 21 22
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 09 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37179
Entity Detected:Tsga10ip, testis specific 10 interacting protein ( MGI:1925556)
Sequence:sense strand is shown

>T37179
GGAGAGCTTCATCATAGATGGCCAGGCTCTGTTTCAGTCAAGTTCCTCTATGGCCATGGGCCATCGACCC
CTGCTCCTCCCTCCAGCAGCCCCCCAGTGCAAGTCCAGGCACAAGTCAGTAGCCAACCTCTCAGAAGACC
TTCGAGCCTGCCACAAGATGGAAGAGCAAAGCGTGGGGAGGAGACATCACCTAGGGGGCTGGGCCAACTT
TCCCCTGCCTCTGAGCAAAGTGGAGAGTCAAGGGCTAGATCAACCTAGCTTCTGGTTATCAGGGAAAGGG
TCAGGATCAGAGTCTGAGGATGTCTTAGAGATAGAAGGCCAGAATTCTGAAGAGGCTGAGAAAAGTCTCA
GCTCTGGGGAACTGCCTCAGCTCCCAGGGCAGAGCTTGATCTTGGAGGAGGAATTGATATCAGAGGCGAT
GGAGGAGGAACACAGCGACCCCCACAAGGGAAAGGGCAGTTCTGTCAATAAGGGGGGGAATTCTGGAGAG
AAGGGCTCAGAAGAGGGGGAGCTGCAGAGCCACAACCAGGGAAGCAGCTCTAGCTTCAACAGTCTCCGAA
AGCCACAGAAGGGGATCTCAAGGGCCAAGGACCTGAAGGGGCCCTGGGACCTGGAGCGGCTACACAGGCA
GTTACAGGAAGAACTGGAATGTGGTCCCCAGAAGCAGACCTGGAAGGCGCTGCGGGCAGCTGTCCAGGCC
TCTGCCAGGAACAAGAAGACCCCCATCTTGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 86306. Forward Primer - name:086306_F_cDNA_Tsga10ip, sequence:GGAGAGCTTCATCATAGATGGC; Reverse Primer - name:086306_N_SP6_cDNA_Tsga10ip, sequence:CCAAGATGGGGGTCTTCTTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18113 same embryo
 EMAGE:18111 same embryo
 EMAGE:18112 same embryo
 EurExpress:euxassay_011930 same experiment
 MGI:4828950 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS