EMAGE mouse gene expression spatial database

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EMAGE:18118

U2af1 U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 ( MGI:98884)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011936. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011936_01	euxassay_011936_02	euxassay_011936_03	euxassay_011936_04

euxassay_011936_05	euxassay_011936_06	euxassay_011936_07	euxassay_011936_08	euxassay_011936_09

euxassay_011936_10	euxassay_011936_11	euxassay_011936_12	euxassay_011936_13	euxassay_011936_14

euxassay_011936_15	euxassay_011936_16	euxassay_011936_17	euxassay_011936_18	euxassay_011936_19

euxassay_011936_20	euxassay_011936_21	euxassay_011936_22	euxassay_011936_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18118_wholemount_strong.wlz

18118_wholemount_moderate.wlz

18118_wholemount_weak.wlz

18118_wholemount_possible.wlz

18118_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18118_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 13 14 15 16 17 18 19 20 21 22

radius

moderate

homogeneous

moderate expression: see section 01

ulna

moderate

homogeneous

moderate expression: see section 01

humerus

moderate

homogeneous

moderate expression: see section 01 02 03 19 20 21 22 23

fibula

moderate

homogeneous

moderate expression: see section 03

tibia

moderate

homogeneous

moderate expression: see section 02 03 04

femur

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

otic capsule

moderate

homogeneous

moderate expression: see section 05 06 07 14 15

naris

moderate

homogeneous

moderate expression: see section 12 13 15 16 17

nasal septum

moderate

homogeneous

moderate expression: see section 13 14

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

meckel's cartilage

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

axial skeleton

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14

basioccipital bone

moderate

homogeneous

moderate expression: see section 02 03 14 15 16 17 18 19 20 21

basisphenoid bone

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 02 03 04 17 18 19 20 21 22

vault of skull

moderate

homogeneous

moderate expression: see section 01

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 18 19 20 23

clavicle

moderate

homogeneous

moderate expression: see section 06 17

scapula

moderate

homogeneous

moderate expression: see section 02 03 18 19

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 09 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37185
Entity Detected:	U2af1, U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 ( MGI:98884)
Sequence:	sense strand is shown >T37185 TAAACCAACCTTTAGCCAGACCATTGCCCTCTTGAACATTTACCGTAACCCTCAAAACTCTTCCCAGTCT GCTGACGGTTTGCGCTGTGCTGTGAGCGACGTGGAGATGCAGGAGCACTATGATGAGTTCTTTGAGGAAG TCTTCACTGAGATGGAAGAGAAGTACGGGGAAGTCGAGGAGATGAACGTCTGCGACAACCTAGGGGACCA CCTGGTGGGGAACGTGTATGTCAAGTTTCGACGTGAGGAGGATGCAGAGAAAGCCGTGATCGACTTGAAT AACCGTTGGTTTAATGGACAGCCGATCCATGCAGAGCTGTCCCCAGTAACTGACTTCAGGGAAGCCTGCT GCCGCCAGTATGAAATGGGAGAGTGCACAAGAGGGGGCTTCTGCAACTTCATGCATTTGAAGCCCATCTC AAGAGAGCTACGACGGGAGCTGTATCGGCGCCGGCGCAAGAAGCATAGATCCAGGTCCCGATCCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91171. Forward Primer - name:091171_F_cDNA_U2af1, sequence:TAAACCAACCTTTAGCCAGACC; Reverse Primer - name:091171_N_SP6_cDNA_U2af1, sequence:TGGATCGGGACCTGGATCTAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18117 same assay
	EMAGE:18116 same embryo
	EMAGE:18114 same embryo
	EurExpress:euxassay_011936 same experiment
	EMAGE:18115 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source