EMAGE mouse gene expression spatial database

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EMAGE:18127

Tyrp1 tyrosinase-related protein 1 ( MGI:98881)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011893. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011893_01	euxassay_011893_02	euxassay_011893_03	euxassay_011893_04

euxassay_011893_05	euxassay_011893_06	euxassay_011893_07	euxassay_011893_08	euxassay_011893_09

euxassay_011893_10	euxassay_011893_11	euxassay_011893_12	euxassay_011893_13	euxassay_011893_14

euxassay_011893_15	euxassay_011893_16	euxassay_011893_17

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18127_wholemount_strong.wlz

18127_wholemount_moderate.wlz

18127_wholemount_weak.wlz

18127_wholemount_possible.wlz

18127_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18127_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 02 03 04 12 13 14

humerus

weak

regional

weak expression: see section 01 16 17

hip

weak

regional

weak expression: see section 05

femur

weak

regional

weak expression: see section 01 02 03 04 13 14 15

ear

strong

spotted

strong expression: see section 02 03 04 05 06 12 13 14 15 16 17 weak expression: see section 01

eye skeletal muscle

strong

spotted

strong expression: see section 02 03 04 15 16 17

heart

strong

spotted

strong expression: see section 07 08 09

meckel's cartilage

weak

regional

weak expression: see section 02 03 04 05 09 11 12 13 14 15

axial skeleton

weak

regional

weak expression: see section 06 07 08 11 12

basisphenoid bone

weak

regional

weak expression: see section 07 08 09 10 11

scapula

weak

regional

weak expression: see section 02 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9568
Entity Detected:	Tyrp1, tyrosinase-related protein 1 ( MGI:98881)
Sequence:	sense strand is shown >T9568 CTGGAAGAGAGGACCAGCCCCTCCCAGTGTGAGGAATCTGGCTTGGGATTTACTGTCTGGCAGAAAATCT CTTCGGGCAATTAACAGCTGGCATCAGGGGAAAAGCAGACATCCAACAACACTAGCTCTGAAGGAGATCA GCAGAGAAACCTTCCAGGGATTCATGGTACTGGTGAGCAGCTCTGTGCTGTATTTTCATCTGAGCACCCC TGTCTTCTCCATGCAAAGAGCAGCATAGGAGACCTGTGTTCTGAACTCTTGCTTCGAGAAGAATGAAATC TTACAACGTCCTCCCCCTAGCCTATATCTCCCTTTTCCTGATGCTGTTTTATCAGGTTTGGGCTCAGTTT CCACGAGAGTGTGCCAATATTGAGGCTCTGAGACGTGGGGTGTGTTGCCCAGACCTGCTCCCTTCCTCTG GACCGGGGACTGACCCTTGTGGCTCATCATCAGGAAGAGGCAGGTGTGTGGCTGTGATTGCAGACTCCCG ACCCCACAGCCGCCATTATCCCCACGATGGTAAAGATGACCGAGAAGCCTGGCCTCTGAGGTTCTTTAAT AGAACATGTCAGTGCAATGATAATTTCTCAGGACACAACTGTGGGACTTGCCGTCCTGGGTGGAGAGGAG CTGCATGCAACCAGAAAATTCTCACAGTCAGGAGAAATCTTCTAGACTTAAGTCCAGAAGAAAAGAGCCA CTTTGTCAGGGCCTTGGATATGGCGAAGCGCACAACTCACCCTCAATTTGTCATTGCCACAAGGAGGTTA GAAGACATACTGGGACCAGATGGCAACACACCACAATTTGAGAACATTTCCGTTTATAACTACTTTGTTT GGACACACTATTATTCAGTCAAAAAAACCTTCCTCGGGACAGGACAGGAAAGCTTTGGGGATGTGGATTT CTCTCACGAAGGACCCGCTTTTCTCACATGGCACAGGTACCATCTGCTGCAGCTGGAGAGAGACATGCAG GAGATGCTGCAGGAGCCTTCTTTCTCCCTTCCTTACTGGAATTTTGCAACTGGGAAAAACGTCTGCGATG TCTGCACTGATGACTTGATGGGATCCAGAAGCAACTTCGATTCTACTCTTATAAGCCCCAACTCTGTCTT TTCTCAATGGAGAGTGGTCTGTGAATCCTTGGAAGAGTACGATACCCTGGGAACACTTTGTAACAGCACT GAGGGTGGACCAATCAGGAGAAACCCAGCTGGAAATGTAGGGAGACCAGCAGTGCAGCGTCTTCCTGAGC CACAGGATGTCACTCAGTGCTTGGAGGTCCGTGTATTTGACACACCTCCTTTTTATTCCAATTCTACAGA CAGTTTTCGAAATACAGTGGAAGGTTACAGTGCTCCCACGGGAAAATATGACCCTGCTGTTCGAAGCCTT CACAACCTGGCCCACCTCTTCCTGAATGGAACGGGAGGACAAACCCATTTGTCTCCCAATGATCCTATTT TTGTCCTCCTGCACACTTTCACTGATGCGGTCTTTGACGAATGGCTAAGGAGGTATAACGCCGATATTTC TACCTTCCCGTTGGAAAACGCACCTATTGGACATAACAGGCAATACAACATGGTGCCATTCTGGCCTCCA GTTACCAACACAGAAATGTTTGTTACTGCTCCAGACAATCTGGGATATGCTTATGAAGTTCAATGGCCAG GTCAGGAGTTTACTGTATCTGAAATCATTACCATTGCTGTAGTGGCTGCGTTGTTACTTGTAGCTGCCAT TTTCGGGGTTGCTTCTTGTCTGATCCGTTCTAGAAGCACCAAGAATGAAGCCAACCAGCCTCTCCTCACT GATCACTATCAACGCTATGCTGAGGACTATGAGGAGCTCCCGAATCCTAACCACTCCATGGTCTGATAGC CGCCTGCCCATCCCCGCACGCAGCAGGTTGGGAAGCAACCTGATTGAAACACAGCTGTCAACCGTATTTC CTTTGGCTTCCTTCTTTTCTTCCCAAGATAGATGTGGTTTGGAATTAAAGCATTGCTCTCCAGTGATGAC AGTTTAGACTCCTTTTAATGGGTTTTAATAATTAGTTCTATTGAAGATGGCAAATTATACCAGTTGTGTG ATATCTGAGAGGATAATTGGATGAACTTGGTGGTATTCTAAAGATGGCCTGTATGCTGTGAAAAAGCAAT GCTTTGTTTACACAGGAAAAATAAGTTTGATGTAAAATCTCTATTTCAAATATATGAGTGCCCATTCTTA TGGAGTTAACATGTGAATGCATTGTTATTGTTGGTACTAATCAAAAGTTATAAAAGCAGCCATGTTGATT AGTTACAACAAATCACATGACATTATAAACAAATAGAATCCTGATTATCTTTCTGAAACTGCTCTCATTT TGCTTTCAGCGTATTATTTGGCCTCAGGTGCAAGGCCACCACAAAGTCACAGTCCTTGCCACACATGGTA AAGACGAAAGCTTCACTTGCTGGAACACATCTGCTCTTGCATATTTGCCAATGTGTTCCCTAGCTCAGTT CTCTGGACATGAAACTGCCTGGGCCACAGTTCACCTCTAATTCTCGTATCTTAGAGGCCTGCGAGGAAGG TATCAGTCAGAGGACACAATGTTCTGTGCATCTTATCAAATGTTGTATTTCATTTCAACAGCATTGTTAC ACCACTTATCAGCCTTCTGTGTATTTCAAAATAAATATAATAAAATCTTCTTTCATTGGTAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30613975 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30613975 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18128 same embryo
	EurExpress:euxassay_011893 same experiment
	MGI:4829042 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source