EMAGE mouse gene expression spatial database

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EMAGE:18130

Fbln1 fibulin 1 ( MGI:95487)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011935. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011935_01	euxassay_011935_02	euxassay_011935_03	euxassay_011935_04

euxassay_011935_05	euxassay_011935_06	euxassay_011935_07	euxassay_011935_08	euxassay_011935_09

euxassay_011935_10	euxassay_011935_11	euxassay_011935_12	euxassay_011935_13	euxassay_011935_14

euxassay_011935_15	euxassay_011935_16	euxassay_011935_17	euxassay_011935_18	euxassay_011935_19

euxassay_011935_20	euxassay_011935_21	euxassay_011935_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18130_wholemount_strong.wlz

18130_wholemount_moderate.wlz

18130_wholemount_weak.wlz

18130_wholemount_possible.wlz

18130_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18130_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon meninges

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

hindbrain meninges

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

spinal cord meninges

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13

nasal cavity

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

heart valve

strong

regional

strong expression: see section 09 10 11 12

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09

midgut

strong

regional

strong expression: see section 09 10 11 12 13 moderate expression: see section 17 weak expression: see section 14 16

bladder

strong

regional

strong expression: see section 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30000
Entity Detected:	Fbln1, fibulin 1 ( MGI:95487)
Sequence:	sense strand is shown >T30000 ACCAGTGTGGGCTGGTGTTCCGTGCCTGCTGTGTGAAGGCCCGGGAGAATTCAGACTTTGTCCAAGGCAA CGGTGCAGACCTTCAGGACCCAGCTAAGATTCCTGACGAGGAGGACCAAGAAGACCCGTACCTGAATGAC CGCTGTCGAGGTGGCGGGCCCTGTAAGCAGCAGTGCCGTGACACTGGGGACGAGGTGATCTGCTCTTGCT TTGTGGGCTACCAGCTGCAGTCGGATGGTGTCTCCTGCGAAGATATTAATGAATGCATCACAGGCAGCCA TAACTGCCGGCTGGGAGAATCCTGCATCAATACAGTGGGCTCTTTCCGCTGCCAGCGGGACAGCAGCTGT GGGACTGGCTATGAGCTCACAGAGGATAATAACTGCAAAGATATTGACGAATGTGAGACTGGTATTCATA ACTGCCCCCCCGATTTTATCTGTCAGAATACTCTGGGATCCTTCCGTTGCAGACCGAAGCTGCAGTGCAA GAGCGGCTTCATACAGGATGCTCTAGGCAACTGCATTGATATCAATGAGTGTTTAAGTATCAGTGCTCCA TGCCCTGTGGGGCAGACATGCATCAATACAGAGGGCTCCTACACATGCCAGAAGAATGTGCCCAACTGTG GCCGTGGTTATCATCTCAATGAAGAGGGGACCCGCTGTGTTGATGTGGATGAGTGCTCCCCACCAGCGGA GCCCTGTGGGAAGGGACACCACTGCCTGAACTCCCCCGGCAGCTTCCGCTGCGAGTGCAAGGCTGGCTTC TATTTTGATGGCATCAGCAGGACCTGCGTGGATATCAACGAGTGCCAGCGCTATCCCGGGCGCCTGTGTG GCCACAAGTGTGAGAACACGCCAGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3495754), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 11336. Forward Primer - name:011336_F_IRAV12-15_A04_Fbln1, sequence:ACCAGTGTGGGCTGGTGT; Reverse Primer - name:011336_R_SP6_IRAV12-15_A04_Fbln1, sequence:AGCCTGGCGTGTTCTCAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18133 same embryo
	EMAGE:18132 same embryo
	EMAGE:18129 same embryo
	EMAGE:18134 same embryo
	EMAGE:18131 same embryo
	EurExpress:euxassay_011935 same experiment
	MGI:4824788 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source