EMAGE mouse gene expression spatial database

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EMAGE:18149

Pfkm phosphofructokinase, muscle ( MGI:97548)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011951. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011951_01	euxassay_011951_02	euxassay_011951_03	euxassay_011951_04

euxassay_011951_05	euxassay_011951_06	euxassay_011951_07	euxassay_011951_08	euxassay_011951_09

euxassay_011951_10	euxassay_011951_11	euxassay_011951_12	euxassay_011951_13	euxassay_011951_14

euxassay_011951_15	euxassay_011951_16	euxassay_011951_17	euxassay_011951_18	euxassay_011951_19

euxassay_011951_20	euxassay_011951_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18149_wholemount_strong.wlz

18149_wholemount_moderate.wlz

18149_wholemount_weak.wlz

18149_wholemount_possible.wlz

18149_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18149_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 12 13

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

pons mantle layer

moderate

regional

moderate expression: see section 06

pons ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 02 03 06 07 14 15 16 17 18 19

vagus x ganglion

moderate

homogeneous

moderate expression: see section 06 14

ventral grey horn

moderate

regional

moderate expression: see section 07 08 09 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30018
Entity Detected:	Pfkm, phosphofructokinase, muscle ( MGI:97548)
Sequence:	sense strand is shown >T30018 CGCGATCTCCAGGTGAATGTTGAACATCTGGTGCAGAAGATGAAAACAACTGTGAAGAGAGGCCTGGTGC TGAGGAATGAGAAATGCAATGAGAACTACACTACTGACTTCATTTTCAACCTGTACTCTGAGGAGGGGAA GGGCATCTTTGACAGCAGGAAGAACGTGCTTGGCCACATGCAGCAGGGTGGAAGCCCAACTCCCTTTGAC AGGAATTTTGCCACTAAGATGGGTGCTAAGGCTATGAACTGGATGTCTGGGAAAATCAAGGAGAGTTACC GTAATGGACGGATCTTTGCCAACACCCCTGACTCAGGCTGCGTTCTGGGGATGCGTAAGAGGGCCCTGGT CTTTCAGCCAGTAACTGAGCTGAAGGACCAGACAGACTTTGAACACCGAATCCCCAAAGAACAGTGGTGG CTGAAGCTGAGGCCAATCCTCAAAATCCTAGCCAAGTACGAGATTGATCTGGACACCTCCGACCACGCCC ACCTGGAGCACATTTCAAGGAAACGGTCTGGAGAAGCCGCCGTCTAGACCTCTCTGGAGTGAGGGGGAAA GATCATGTGACCATGGTCAGCTCACACCCTGGTAGATCATGGCTTCTCAGTGTTCTAGCCCAGCCCCTCC CCTTCTAGGTTTCCCTGTACTCCATGCCTGCAACCAGGATCAGCTGTGGCCAGGAGCTGGAGGAGGGGCG GTGAGTGACTCTCCTAGGTGGCTGTCCTTCCTCCCTTCCACTCCAGCTTCATCGGTCACACAGGCTGGGT CTCTCTAGTGCTACGGCTAGATTTCAGCTACTCAAGAGTTTTCCTAAAAATAAGCTTTATTTATTTCTTT GTGATAACAAAGAGTCTTAGGTCCCTTTACTCGCACCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3495173), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 14082. Forward Primer - name:014082_F_IRAV12-15_G01_Pfkm, sequence:CGCGATCTCCAGGTGAAT; Reverse Primer - name:014082_R_SP6_IRAV12-15_G01_Pfkm, sequence:TGTGGTGCGAGTAAAGGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18146 same embryo
	EMAGE:18147 same embryo
	EMAGE:18148 same embryo
	EurExpress:euxassay_011951 same experiment
	MGI:4827165 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source