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EMAGE:18204

Kdm5a lysine (K)-specific demethylase 5A ( MGI:2136980)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18204 EMAGE:18204 EMAGE:18204 EMAGE:18204 EMAGE:18204
"Pseudo-wholemount" of euxassay_011985. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011985_01 euxassay_011985_02 euxassay_011985_03 euxassay_011985_04
EMAGE:18204 EMAGE:18204 EMAGE:18204 EMAGE:18204 EMAGE:18204
euxassay_011985_05 euxassay_011985_06 euxassay_011985_07 euxassay_011985_08 euxassay_011985_09
EMAGE:18204 EMAGE:18204 EMAGE:18204 EMAGE:18204 EMAGE:18204
euxassay_011985_10 euxassay_011985_11 euxassay_011985_12 euxassay_011985_13 euxassay_011985_14
EMAGE:18204 EMAGE:18204 EMAGE:18204 EMAGE:18204 EMAGE:18204
euxassay_011985_15 euxassay_011985_16 euxassay_011985_17 euxassay_011985_18 euxassay_011985_19
EMAGE:18204 EMAGE:18204 EMAGE:18204 EMAGE:18204 EMAGE:18204
euxassay_011985_20 euxassay_011985_21 euxassay_011985_22 euxassay_011985_23 euxassay_011985_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18204Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18204_wholemount_strong.wlz
18204_wholemount_moderate.wlz
18204_wholemount_weak.wlz
18204_wholemount_possible.wlz
18204_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18204_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
strong strong
regionalstrong expression: see section 05 06 07 08 weak expression: see section 15 16 17
vibrissa
strong strong
regionalstrong expression: see section 04 05 06 07 moderate expression: see section 08 21 22 23
cornea epithelium
strong strong
regionalstrong expression: see section 03 04
oral epithelium
strong strong
regionalstrong expression: see section 05
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30061
Entity Detected:Kdm5a, lysine (K)-specific demethylase 5A ( MGI:2136980)
Sequence:sense strand is shown

>T30061
ATTTGTGCTCTTGCCCCATGCAGAACAAATGTCTTAGGTACCGCTATCCACTAGAAGACCTTCCATCTCT
GCTTTATGGTGTCAAAGTGAGAGCACAATCCTATGACACTTGGGTCAATCGTGTTACAGAAGCATTGTCA
GCTAGCTTCAACCATAAGAAAGATTTGATTGAATTGAGAGTAATGCTGGAAGATGCTGAGGATAGGAAAT
ACCCAGAGAATGATCTCTTTCGAAAGCTCAGGGACGCGGTGAAAGAAGCTGAGACCTGTGGTTCTGTGGC
TCAGCTGCTTCTGAGCAAAAAGCAAAAACACAGAATCTGCTGGATGATGTAGAAGAATTTCATGAACGTG
CCCAGGAGGCCATGATGGATGAAACCCCAGATTCTTCCAAACTCCAAATGTTAATAGACATGGGCTCTAG
TCTCTATGTGGAGCTACCTGAATTGCCCCGACTAAAGCAAGAGCTCCAGCAGGCTCGATGGCTGGATGAA
GTAAGGCTAACTCTATCAGATCCACAACAAGTCACTTTGGATGTCATGAAGAAATTGATTGACTCTGGCG
TAGGCTTGGCACCTCACCATGCTGTAGAAAAAGCAATGGCCGAACTACAGGAACTCCTTACAGTCTCTGA
ACGATGGGAAGAGAAGGCTAAGGTCTGCCTGCAAGCAAGACCTCGGCACAGTATGGCAAATTTAGAAAAT
ATTGTGAATGAAGCCAAGAACATTCCAGCCTTTCTACCCAATGTGTTGTCCTTAAAAGAAGCCTTACAAA
AGGCTCGTGAATGGACAGCTAAAGTGGAAGCTATTCAGAGTGGCAACAACTATGCTTACTTGGAACAGCT
TGAGAGCCTATCTGCTAAAGGACGCCCTATCCCTGTGCGTCTTGATGCCTTGCCTCAAGTGGAGTCACAG
GTAGCAGCAGCACGGGCCTGGAGAGAACGTACTGGGCGGACCTTCCTTAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3603073), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15690. Forward Primer - name:015690_F_IRAV20-23_C08_Jarid1a, sequence:ATTTGTGCTCTTGCCCCA; Reverse Primer - name:015690_R_SP6_IRAV20-23_C08_Jarid1a, sequence:TTTAAGGAAGGTCCGCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18201 same embryo
 EMAGE:18203 same embryo
 EMAGE:18200 same embryo
 EMAGE:18205 same embryo
 EMAGE:18202 same embryo
 EurExpress:euxassay_011985 same experiment
 MGI:4825746 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS