EMAGE mouse gene expression spatial database

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EMAGE:18207

Darc Duffy blood group, chemokine receptor ( MGI:1097689)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011982. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011982_01	euxassay_011982_02	euxassay_011982_03	euxassay_011982_04

euxassay_011982_05	euxassay_011982_06	euxassay_011982_07	euxassay_011982_08	euxassay_011982_09

euxassay_011982_10	euxassay_011982_11	euxassay_011982_12	euxassay_011982_13	euxassay_011982_14

euxassay_011982_15	euxassay_011982_16	euxassay_011982_17	euxassay_011982_18	euxassay_011982_19

euxassay_011982_20	euxassay_011982_21	euxassay_011982_22	euxassay_011982_23	euxassay_011982_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18207_wholemount_strong.wlz

18207_wholemount_moderate.wlz

18207_wholemount_weak.wlz

18207_wholemount_possible.wlz

18207_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18207_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

homogeneous

moderate expression: see section 03 04 05 06 18

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 04 05

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 17 18 19 20

vagus x ganglion

moderate

homogeneous

moderate expression: see section 06

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 05 06 17

trigeminal v nerve

moderate

homogeneous

moderate expression: see section 09 10 17

spinal cord

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

dorsal root ganglion

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 13 14 15 16

eyelid

moderate

regional

moderate expression: see section 01 02 03 04 05

neural retina

moderate

regional

moderate expression: see section 03 04 05 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30064
Entity Detected:	Darc, Duffy blood group, chemokine receptor ( MGI:1097689)
Sequence:	sense strand is shown >T30064 GCCACAGCTGCTGACAGAGTCCAGGCCCTGTACTTCTCTGCCCTGAGCCTGCAGTGCCATGGGGAACTGT CTGTATCCGGTGGAAACCCTTTCACTAGACAAGAATGGAACTCAGTTTACTTTCGACAGCTGGAATTATT CGTTTGAAGATAACTACTCCTATGAACTCTCGAGTGACTACAGCCTGACACCAGCTGCTCCCTGCTACTC CTGTAATCTGCTTGACAGGTCTTCCCTGCCCTTCTTCATGCTCACCAGTGTCCTGGGCATGCTGGCAAGT GGCAGCATCCTCTTCGCGATTCTCAGACCTTTCTTCCACTGGCAGATTTGCCCCAGCTGGCCCATTCTGG CAGAGTTAGCAGTGGGCAGTGCCCTGTTCAGCATTGCAGTGCCCATCCTGGCACCAGGCTTACACAGCGC CCACAGCACAGCCCTATGCAACCTGGGCTACTGGGTATGGTATACTTCTGCTTTTGCCCAAGCTCTGTTG ATAGGATGCTATGCTTGCCTGAATCCCAGACTGAATATTGGTCAACTCCGTGGCTTCACCTTGGGACTCA GTGTGGGACTTTGGGGAGCAGCTGCCCTCTCAGGGCTGCCAGTCGCCCTGGCCAGTGATGTTTACAATGG CTTCTGCACCTTTCCATCCTCCAGAGACATGGAAGCTTTGAAGTACACTCATTATGCCATCTGTTTTACC ATCTTCACTGTATTGCCACTGACTCTTTTGGCAGCCAAGGGGCTGAAGATAGCACTGAGCAAGGGGCCTG GCCCCTGGGTTAGTGTCTTGTGGATCTGGTTCATTTTCTGGTGGCCTCATGGGATGGTTCTCATATTTGA TGCTTTGGTGAGGTCCAAAACTGTTCTTCTGTATACATGTCAATCCCAGAAGATTCTTGATGCGATGCTG AATGTGACAGAAGCCCTGAGTATGCTGCACTGTGTGGCTACCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3968984), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 8984. Forward Primer - name:008984_F_IRAV20-23_C18_Dfy, sequence:GCCACAGCTGCTGACAGA; Reverse Primer - name:008984_R_SP6_IRAV20-23_C18_Dfy, sequence:GGGGTAGCCACACAGTGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18206 same embryo
	EMAGE:18208 same embryo
	EMAGE:18209 same embryo
	EurExpress:euxassay_011982 same experiment
	MGI:4824234 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source