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EMAGE:18211

Clca1 chloride channel calcium activated 1 ( MGI:1316732)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18211 EMAGE:18211 EMAGE:18211 EMAGE:18211 EMAGE:18211
"Pseudo-wholemount" of euxassay_011993. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011993_01 euxassay_011993_02 euxassay_011993_03 euxassay_011993_04
EMAGE:18211 EMAGE:18211 EMAGE:18211 EMAGE:18211 EMAGE:18211
euxassay_011993_05 euxassay_011993_06 euxassay_011993_07 euxassay_011993_08 euxassay_011993_09
EMAGE:18211 EMAGE:18211 EMAGE:18211 EMAGE:18211 EMAGE:18211
euxassay_011993_10 euxassay_011993_11 euxassay_011993_12 euxassay_011993_13 euxassay_011993_14
EMAGE:18211 EMAGE:18211 EMAGE:18211 EMAGE:18211 EMAGE:18211
euxassay_011993_15 euxassay_011993_16 euxassay_011993_17 euxassay_011993_18 euxassay_011993_19
EMAGE:18211 EMAGE:18211 EMAGE:18211 EMAGE:18211
euxassay_011993_20 euxassay_011993_21 euxassay_011993_22 euxassay_011993_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18211Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18211_wholemount_strong.wlz
18211_wholemount_moderate.wlz
18211_wholemount_weak.wlz
18211_wholemount_possible.wlz
18211_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18211_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 15 16 17 18 19 20 21
radius
moderate moderate
regionalmoderate expression: see section 01 02
ulna
moderate moderate
regionalmoderate expression: see section 02
humerus
moderate moderate
regionalmoderate expression: see section 01 02 03 04 19 20 21 22 23
hindlimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 06 23
hindlimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 06 23
hindlimb digit 4 phalanx
moderate moderate
regionalmoderate expression: see section 23
fibula
moderate moderate
regionalmoderate expression: see section 03 04 05
tibia
moderate moderate
regionalmoderate expression: see section 03 04 05
femur
moderate moderate
regionalmoderate expression: see section 03 04 06 07 08 17 18 19 20 21 22 23
otic capsule
moderate moderate
regionalmoderate expression: see section 06 07 08 15 16
naris
moderate moderate
regionalmoderate expression: see section 12 13 15 16
nasal septum
moderate moderate
regionalmoderate expression: see section 13 14
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
aorta
strong strong
regionalstrong expression: see section 08 09 10 11
urinary system
strong strong
regionalstrong expression: see section 03 04 05 06 07
bladder
strong strong
regionalstrong expression: see section 11 12 13 14
testis
strong strong
regionalstrong expression: see section 05 06 07 17 18 19
axial skeleton
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14
basioccipital bone
moderate moderate
regionalmoderate expression: see section 01 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 04 08 09 10 11 12 13 14 15
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 01 02 03 19 20 21 22 23
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 18 19 20 21 22 23
scapula
moderate moderate
regionalmoderate expression: see section 01 19
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 09 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30074
Entity Detected:Clca1, chloride channel calcium activated 1 ( MGI:1316732)
Sequence:sense strand is shown

>T30074
AGGGCTGCAGGTCCTTCTGTTCCTCACCCTGCATCTCCTGCAGAACACAGAGAGCTCCATGGTGCATCTC
AACAGCAATGGATACGAGGGTGTGGTCATTGCCATTAACCCCAGTGTGCCGGAGGACGAAAGGCTCATCC
CAAGCATAAAGGAAATGGTAACTCAAGCCTCTACCTACCTGTTTGAAGCCAGCCAAGGAAGAGTTTATTT
CAGAAACATAAGCATATTAGTCCCGATGACCTGGAAGTCAAAACCTGAGTACTTAATGCCAAAACGAGAA
TCGTATGACAAAGCAGACGTCATAGTTGCGGATCCTCACCTGCAACATGGAGACGACCCCTACACCCTTC
AGTATGGACAGTGTGGGGACAGAGGACAGTACATACACTTCACTCCAAACTTCCTACTCACTGATAACTT
GCGTATCTACGGACCCCGAGGCAGAGTCTTTGTCCATGAGTGGGCCCATCTCCGGTGGGGAGTATTTGAT
GAGTATAACGTGGACCAGCCTTTCTACATGTCTAGAAAGAACACTATAGAAGCAACAAGGTGTTCCACCA
GGATCACTGGCACCAATGTGGTTCACAACTGTGAGAGAGGCAACTGTGTCACAAGGGCGTGCCGGCGTGA
CTCGAAGACACGGCTGTATGAACCCAAATGTACATTTATCCCAGACAAAATACAGACGGCTGGGGCCTCC
ATAATGTTCATGCAAAACCTTAATTCTGTGGTTGAATTTTGCACAGAAAAAAACCACAATGCAGAAGCCC
CAAACCTACAAAACAAAATGTGCAATCGCAGAAGCACGTGGGATGTAATCAAGACGTCTGCTGACTTTCA
GAATGCCCCTCCCATGAGAGGAACAGAAGCCCCTCCTCCACCTACATTTTCACTGCTCAAGTCCAGAAGG
CGAGTGGTGTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3710379), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 35157. Forward Primer - name:035157_F_IRAV20-23_E15_Clca1, sequence:AGGGCTGCAGGTCCTTCT; Reverse Primer - name:035157_R_SP6_IRAV20-23_E15_Clca1, sequence:GCACACCACTCGCCTTCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18214 same embryo
 EMAGE:18212 same embryo
 EMAGE:18213 same embryo
 EMAGE:18210 same embryo
 EurExpress:euxassay_011993 same experiment
 MGI:4823892 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS