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EMAGE:18232

Psmd9 proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 ( MGI:1914401)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18232 EMAGE:18232 EMAGE:18232 EMAGE:18232 EMAGE:18232
"Pseudo-wholemount" of euxassay_012010. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012010_01 euxassay_012010_02 euxassay_012010_03 euxassay_012010_04
EMAGE:18232 EMAGE:18232 EMAGE:18232 EMAGE:18232 EMAGE:18232
euxassay_012010_05 euxassay_012010_06 euxassay_012010_07 euxassay_012010_08 euxassay_012010_09
EMAGE:18232 EMAGE:18232 EMAGE:18232 EMAGE:18232 EMAGE:18232
euxassay_012010_10 euxassay_012010_11 euxassay_012010_12 euxassay_012010_13 euxassay_012010_14
EMAGE:18232 EMAGE:18232 EMAGE:18232 EMAGE:18232 EMAGE:18232
euxassay_012010_15 euxassay_012010_16 euxassay_012010_17 euxassay_012010_18 euxassay_012010_19
EMAGE:18232
euxassay_012010_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18232Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18232_wholemount_strong.wlz
18232_wholemount_moderate.wlz
18232_wholemount_weak.wlz
18232_wholemount_possible.wlz
18232_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18232_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
exoccipital bone
weak weak
regionalweak expression: see section 01 02 18 19 20
temporal bone petrous part
weak weak
regionalweak expression: see section 01 18 19 20
orbito-sphenoid
weak weak
regionalweak expression: see section 01 02 03 04 05 06 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30672
Entity Detected:Psmd9, proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 ( MGI:1914401)
Sequence:sense strand is shown

>T30672
GCGATGTCGGGTGAAGACGTTCCGCACCGGGCAGAGTCCTCCGAGGCCCGTGCGGCCGCGGTCAGCGACA
TCCAGGATCTGATGCGACGCAAGGAGGAGATCGAGGCGGAGATCAAGGCTAATTACGACGTCCTGGAGAG
CCAAAAAGGAATTGGCATGAACGAACCGCTGGTGGACTGTGAGGGCTATCCCCGGGCGGATGTGGACTTG
TACCAGGTCCGAACAGCAAGGCACAACATCATCTGTCTCCAGAATGATCACAAGGCACTGATGAAGCAAG
TGGAGGAGGCCCTGCACCAGCTGCACGCTCGGGACAAAGAGAAGCAGGCTCGGGACATGGCTGAAGCCCG
AGAAGAGGCCATGAACCGCAGGCTGGCCTCCAACAGCCCCGTCCTGCCCCAGGCCTTTGCCAGAGTGAAC
AGTATCAGCCCCGGTTCCCCAGCCAGTATTGCGGGCCTGCAAGTGGATGACGAAATTGTGGAGTTCGGCT
CCGTGAACACCCAAAACTTCCAGTCAGTGCAGAACGTGGGCACTGTGGTGCAGCATAGCGAGGGGAAGCC
CCTGAATGTGACGGTGATCCGCAGAGGAGAGAAGCACCAGCTCAGACTGATTCCAACCCGCTGGGCAGGA
AAAGGACTGCTGGGCTGCAACATTATTCCTCTCCAGAGATGACTGTTTCCTGGGATCTGCCTGCAGGAAG
CTGCCTCAGCTGGCCCCGTGCTTGGGCCTGGAGGCGTTTCCTCGTTCTCTAGGCTCCCTTAAGTGTAAGG
ATCTGGAGAAGAATGGTCGAAGCCTGGGCATCGAGGTGGAAGAGACGCTTTGGCTGCCTGATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6819088), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59643. Forward Primer - name:059643_F_IRAV117_e07_Psmd9, sequence:GCGATGTCGGGTGAAGAC; Reverse Primer - name:059643_R_SP6_IRAV117_e07_Psmd9, sequence:ACATCAGGCAGCCAAAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18233 same embryo
 EMAGE:18230 same embryo
 EMAGE:18229 same embryo
 EMAGE:18228 same embryo
 EMAGE:18231 same embryo
 EurExpress:euxassay_012010 same experiment
 MGI:4827490 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS