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EMAGE:18269

Klf9 Kruppel-like factor 9 ( MGI:1333856)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18269 EMAGE:18269 EMAGE:18269 EMAGE:18269 EMAGE:18269
"Pseudo-wholemount" of euxassay_012054. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012054_01 euxassay_012054_02 euxassay_012054_03 euxassay_012054_04
EMAGE:18269 EMAGE:18269 EMAGE:18269 EMAGE:18269 EMAGE:18269
euxassay_012054_05 euxassay_012054_06 euxassay_012054_07 euxassay_012054_08 euxassay_012054_09
EMAGE:18269 EMAGE:18269 EMAGE:18269 EMAGE:18269 EMAGE:18269
euxassay_012054_10 euxassay_012054_11 euxassay_012054_12 euxassay_012054_13 euxassay_012054_14
EMAGE:18269 EMAGE:18269 EMAGE:18269 EMAGE:18269 EMAGE:18269
euxassay_012054_15 euxassay_012054_16 euxassay_012054_17 euxassay_012054_18 euxassay_012054_19
EMAGE:18269 EMAGE:18269 EMAGE:18269 EMAGE:18269 EMAGE:18269
euxassay_012054_20 euxassay_012054_21 euxassay_012054_22 euxassay_012054_23 euxassay_012054_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18269Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18269_wholemount_strong.wlz
18269_wholemount_moderate.wlz
18269_wholemount_weak.wlz
18269_wholemount_possible.wlz
18269_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18269_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 14 15 16
humerus
moderate moderate
regionalmoderate expression: see section 02 weak expression: see section 01 03 04
fibula
moderate moderate
regionalmoderate expression: see section 02 03
tibia
moderate moderate
regionalmoderate expression: see section 01 02 03 05 weak expression: see section 04
femur
moderate moderate
regionalmoderate expression: see section 03 05 06 weak expression: see section 04 07
otic capsule
weak weak
regionalweak expression: see section 07 08 09 15 16
meckel's cartilage
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16
axial skeleton
weak weak
regionalweak expression: see section 07 08 09 10 14 15 16
basioccipital bone
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 12 13 14 15 16
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 12 13 14 15 16
exoccipital bone
moderate moderate
regionalmoderate expression: see section 04 05 06
temporal bone
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 weak expression: see section 22 23 24
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 02 06 07 08 weak expression: see section 23 24
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
pelvic girdle skeleton
weak weak
regionalweak expression: see section 09 10 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35422
Entity Detected:Klf9, Kruppel-like factor 9 ( MGI:1333856)
Sequence:sense strand is shown

>T35422
CATGGTAGCCCTAAGGTCAAAGTCAACATTTGGTCCCCTTGCAGAGGCAACTCTGAACCGTCTCTGACTG
TTGGAGGGCAGGCAAATAAATGCTTTTGGTATTTTCTCCTTTGTTTTTGGGGGAGGGGGGAGGGGGCAAG
GCCAAGACGAGGGGTAGGATTCTGAAGATTCAGACTGGTGGTGTACATACGTCTGACTGGGTGAGTTGAC
CCCTGGCCTCCCACAGTGACTCTGGATCCTTGATGCTTGCTGACTCTCAGAATTGTTTTTTCTTCTCATT
TAATGTAACGACCGGTGTGCTTCAGTTTGTTTAGCAGAACCACTCTCTTGAATCACATTAACTTTTGAGA
TTTAAAAAAAACAAAACAAAAAAAAAAAAAACAACAAAAAAAAACCAACCCTCCATAGCACAGCTGTCTT
TTATGCAAGCAAGAGCACACCTACTCCAGCATGATTTGTCATCTAAAGACTTGAAAACAAAACAACAACA
ACAAAAAGTTACTTATAGTCAATGGATAAGCAGAGTCCGAATTTACACTAATCAAGACAGACCTTCGAGG
GGTCACGATAAGTCCGGAACTTTCAAACCTTGCTTCGTATGAATTGTACTATCTGAACATAAACTGCACT
TTTATTTTCTAATACCGAGGGTGAATACGGTAAATACATGCTTTGAGGGTAGAAGCCGACGGTCTGTTTG
GCACCACGTTATAATCTGCTTATTTTAACAGTATACACATTTCCCTGAGAACCTATGGCAAAGATGGGAG
GGCAGAACATATGAAACACATGCTGACGAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101624. Forward Primer - name:101624_F_cDNA_Bteb1, sequence:CATGGTAGCCCTAAGGTCAAAG; Reverse Primer - name:101624_N_SP6_cDNA_Bteb1, sequence:CCTCGTCAGCATGTGTTTCAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18268 same embryo
 EurExpress:euxassay_012054 same experiment
 MGI:4825787 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS