EMAGE mouse gene expression spatial database

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EMAGE:18277

Inpp4b inositol polyphosphate-4-phosphatase, type II ( MGI:2158925)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012070. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012070_01	euxassay_012070_02	euxassay_012070_03	euxassay_012070_04

euxassay_012070_05	euxassay_012070_06	euxassay_012070_07	euxassay_012070_08	euxassay_012070_09

euxassay_012070_10	euxassay_012070_11	euxassay_012070_12	euxassay_012070_13	euxassay_012070_14

euxassay_012070_15	euxassay_012070_16	euxassay_012070_17	euxassay_012070_18	euxassay_012070_19

euxassay_012070_20	euxassay_012070_21	euxassay_012070_22	euxassay_012070_23	euxassay_012070_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18277_wholemount_strong.wlz

18277_wholemount_moderate.wlz

18277_wholemount_weak.wlz

18277_wholemount_possible.wlz

18277_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18277_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 08 09 14 15

telencephalon mantle layer

weak

regional

weak expression: see section 06 07 08 19 20 21 22

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 06 07 14 15 16

rest of cerebellum mantle layer

weak

regional

weak expression: see section 06 07 15 16

pons mantle layer

weak

regional

weak expression: see section 06 07 15 16

midbrain mantle layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15

tongue muscle

moderate

regional

moderate expression: see section 12 13 14 15 16

genital tubercle of male

moderate

regional

moderate expression: see section 11 12 13 14 15

tail mesenchyme

moderate

regional

moderate expression: see section 11 12 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30699
Entity Detected:	Inpp4b, inositol polyphosphate-4-phosphatase, type II ( MGI:2158925)
Sequence:	sense strand is shown >T30699 ACACAGCGAAGGCAAAGGAAGTTCTCAGCAGCATCAATCAGCTACAACCTCTCATAGCAACTCATGCAGA CCTGCTGCTAACCTCTGCCAGCCAGCGTTCTCCAGACAGCCTGAAGAGCTCTTTAAAGCTGCTTTCAGAA AAAACTGAGCTTTTTGTCCATGCCTTCAAGGACCAGCTGGTCAGGAGTGCTCTTTTAGCTCTCTACACTG CAAGGCCAGGAGGCATCCTTAAGAAGCCACCCTCTCCTAATGTCAGCACAGAGGAGAAAAGTACTCAGCA TGATACCCCACAGCTTAGAAGACAAGACTCCATACCACATCATTCTGACTATGATGAAGAAGAGTGGGAT AGGGTGTGGGCCAACGTGGGGAAGAGCCTGAACTGCATTATCGCTAAAGTAGACAAACTGATCGAGAGAG ACAGTCACAACGAAGAAGGTGCTGGAGGCAGCAGTAGCAAGGATGGAGAGGCAGACCATACCCTGGAAGA TTCCATCACATCTCACCCACGTGAGGACTGGTATGAACAGCTGCACCCACTCATCCTTACCTTGAAGGAG TGCATGGGAGAAGTGGTGAACCGAGCAAAGCAATCCCTGACATTTGTACTGCTTCAGGAACTGGCCTACA GTCTGCCCCAGTGTTTGATGCTGACTCTGAGAAGAGACATTGTCTTCAGTCAGGCGCTTGCTGGATTGGT TTGTGGTTTTATCATCAAATTACATACAAGTTTGCATGACCCTGGCTTCCTACAGCAGCTTCATACAGTG GGCTTGATAGTACAATACGAAGGACTGCTAAGTACTTACAGTGATGAAATTGGAATGCTGGAGGACATGG CAGTCGGCATT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6826283), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59814. Forward Primer - name:059814_F_IRAV118_f08_Inpp4b, sequence:ACACAGCGAAGGCAAAGG; Reverse Primer - name:059814_R_SP6_IRAV118_f08_Inpp4b, sequence:AAATGCCGACTGCCATGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18278 same embryo
	EMAGE:18275 same embryo
	EMAGE:18276 same embryo
	EMAGE:18279 same embryo
	EurExpress:euxassay_012070 same experiment
	MGI:4825594 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source