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EMAGE:18281

Kif5a kinesin family member 5A ( MGI:109564)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18281 EMAGE:18281 EMAGE:18281 EMAGE:18281 EMAGE:18281
"Pseudo-wholemount" of euxassay_012072. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012072_01 euxassay_012072_02 euxassay_012072_03 euxassay_012072_04
EMAGE:18281 EMAGE:18281 EMAGE:18281 EMAGE:18281 EMAGE:18281
euxassay_012072_05 euxassay_012072_06 euxassay_012072_07 euxassay_012072_08 euxassay_012072_09
EMAGE:18281 EMAGE:18281 EMAGE:18281 EMAGE:18281 EMAGE:18281
euxassay_012072_10 euxassay_012072_11 euxassay_012072_12 euxassay_012072_13 euxassay_012072_14
EMAGE:18281 EMAGE:18281 EMAGE:18281 EMAGE:18281 EMAGE:18281
euxassay_012072_15 euxassay_012072_16 euxassay_012072_17 euxassay_012072_18 euxassay_012072_19
EMAGE:18281 EMAGE:18281 EMAGE:18281 EMAGE:18281 EMAGE:18281
euxassay_012072_20 euxassay_012072_21 euxassay_012072_22 euxassay_012072_23 euxassay_012072_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18281Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18281_wholemount_strong.wlz
18281_wholemount_moderate.wlz
18281_wholemount_weak.wlz
18281_wholemount_possible.wlz
18281_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18281_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 05 moderate expression: see section 02 03 04 06 07 08 weak expression: see section 09 10 21 22
radius
strong strong
regionalstrong expression: see section 01 02
humerus
strong strong
regionalstrong expression: see section 01 02 03 weak expression: see section 21 22
fibula
strong strong
regionalstrong expression: see section 01 02
tibia
strong strong
regionalstrong expression: see section 01 02 03 moderate expression: see section 04
femur
strong strong
regionalstrong expression: see section 01 02 03 moderate expression: see section 04 weak expression: see section 15 16 17 18 19 20
brain
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
facial vii ganglion
strong strong
regionalstrong expression: see section 03 04 05 17 18 19
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 04 05 15 16
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 16 17 18 19 20 21
vagus x ganglion
strong strong
regionalstrong expression: see section 06 15
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 05 06 15 16
trigeminal v nerve
strong strong
regionalstrong expression: see section 09 16 17
spinal cord
moderate moderate
homogeneousmoderate expression: see section 03 04 05 06 07 08 09 10 11
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 13 moderate expression: see section 07
cervical ganglion
strong strong
regionalstrong expression: see section 14 moderate expression: see section 07
thoracic ganglion
strong strong
regionalstrong expression: see section 09 moderate expression: see section 07 08
dorsal root ganglion
strong strong
regionalstrong expression: see section 04 05 06 09 10 11 12 13 14
neural retina
strong strong
regionalstrong expression: see section 02 03 04 23 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 weak expression: see section 10 17 18 19 20
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 13 16
meckel's cartilage
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 weak expression: see section 09 13 14 15 17 21
axial skeleton
strong strong
regionalstrong expression: see section 04 05 moderate expression: see section 06 07 08
basioccipital bone
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08
exoccipital bone
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 03 weak expression: see section 23
temporal bone
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 03 weak expression: see section 21 22
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 03 06 07 08 weak expression: see section 21 22 23
scapula
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 03 weak expression: see section 19
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 05 06 weak expression: see section 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30700
Entity Detected:Kif5a, kinesin family member 5A ( MGI:109564)
Sequence:sense strand is shown

>T30700
AGCTGAGCTCTGCGAGGAGACCCCTGTGAATGACAACTCATCCATTGTGGTACGCATCGCACCTGAGGAA
AGGCAGAAATATGAGGAAGAGATCCGCCGTCTCTACAAGCAGCTTGATGACAAGGATGATGAGATCAACC
AGCAGAGCCAGCTCATTGAGAAGCTGAAGCAGCAGATGCTGGACCAGGAAGAGCTGCTCGTGTCCACTCG
GGGAGACAACGAGAAGGTCCAGCGGGAGCTTAGCCACCTGCAGTCCGAGAACGATGCTGCGAAGGACGAG
GTGAAGGAAGTGCTGCAGGCCCTAGAGGAGCTGGCGGTCAACTACGACCAGAAGTCCCAGGAGGTGGAGG
AGAAGAGCCAGCAGAACCAGCTGCTGGTGGACGAGCTGTCCCAGAAAGTGGCCACCATGCTGTCCCTGGA
GTCCGAGCTACAGCGGCTCCAGGAGGTCAGTGGACACCAGCGAAAGCGGATCGCTGAGGTGCTGAATGGG
CTGATGAGGGACCTGAGTGAGTTCAGTGTCATCGTGGGCAACGGCGAGATTAAGCTGCCCGTGGAGATCA
GTGGGGCCATCGAGGAGGAGTTCACGGTGGCCCGGCTCTACATCAGCAAGATCAAGTCGGAGGTGAAGTC
CGTGGTTAAGCGATGTCGGCAGCTGGAGAACCTCCAGGTGGAGTGTCATCGCAAGATGGAGGTGACCGGT
AGGGAGCTGTCATCTTGCCAACTGCTCATCTCACAGCATGAGGCCAAGATCCGTTCACTCACGGAGTACA
TGCAGACTGTGGAGTTGAAGAAACGGCACCTGGAAGAGTCCTACGACTCCCTGAGCGAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6824963), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59750. Forward Primer - name:059750_F_IRAV118_f10_Kif5a, sequence:AGCTGAGCTCTGCGAGGA; Reverse Primer - name:059750_R_SP6_IRAV118_f10_Kif5a, sequence:CATCGCTCAGGGAGTCGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18280 same embryo
 EMAGE:18282 same embryo
 EMAGE:18284 same embryo
 EMAGE:18283 same embryo
 EurExpress:euxassay_012072 same experiment
 MGI:4825767 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS