EMAGE mouse gene expression spatial database

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EMAGE:18298

2310057M21Rik RIKEN cDNA 2310057M21 gene ( MGI:1915527)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012086. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012086_01	euxassay_012086_02	euxassay_012086_03	euxassay_012086_04

euxassay_012086_05	euxassay_012086_06	euxassay_012086_07	euxassay_012086_08	euxassay_012086_09

euxassay_012086_10	euxassay_012086_11	euxassay_012086_12	euxassay_012086_13	euxassay_012086_14

euxassay_012086_15	euxassay_012086_16	euxassay_012086_17	euxassay_012086_18	euxassay_012086_19

euxassay_012086_20	euxassay_012086_21	euxassay_012086_22	euxassay_012086_23	euxassay_012086_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18298_wholemount_strong.wlz

18298_wholemount_moderate.wlz

18298_wholemount_weak.wlz

18298_wholemount_possible.wlz

18298_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18298_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 02 09 10 11 12 16 17 18 19 moderate expression: see section 01 03 04 05 06 07 08 20 21 weak expression: see section 22 23 24

radius

strong

regional

strong expression: see section 01

humerus

strong

regional

strong expression: see section 01 02 03 04 05 moderate expression: see section 22 23 24

hindlimb digit 2 metatarsal

strong

regional

strong expression: see section 05

hindlimb digit 3 metatarsal

strong

regional

strong expression: see section 05

hindlimb digit 4 metatarsal

strong

regional

strong expression: see section 05

tarsus

strong

regional

strong expression: see section 04

fibula

strong

regional

strong expression: see section 02

tibia

strong

regional

strong expression: see section 01 02 03

femur

strong

regional

strong expression: see section 02 03 04 05 06 07 20 21 moderate expression: see section 08 18 19 22 23 24

otic capsule

strong

regional

strong expression: see section 10 16 moderate expression: see section 07 08 09 weak expression: see section 17 18

neural retina

weak

regional

weak expression: see section 01 02 03 22 23 24

meckel's cartilage

strong

regional

strong expression: see section 04 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 03 05 12

axial skeleton

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

basioccipital bone

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 moderate expression: see section 08

basisphenoid bone

strong

regional

strong expression: see section 10 11 12 13 14 16

exoccipital bone

strong

regional

strong expression: see section 02 03 06 19 20 21 moderate expression: see section 01 04 05 07 23 24

temporal bone

strong

regional

strong expression: see section 02 03 06 20 21 22 moderate expression: see section 01 04 05 07 23 24

orbito-sphenoid

strong

regional

strong expression: see section 02 03 06 18 19 20 moderate expression: see section 01 04 05 07 23 24

viscerocranium

strong

regional

Expression in the turbinate bone.

scapula

strong

regional

strong expression: see section 04 05 moderate expression: see section 22 23 24

pelvic girdle skeleton

strong

regional

strong expression: see section 10 16 moderate expression: see section 09 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T107
Entity Detected:	2310057M21Rik, RIKEN cDNA 2310057M21 gene ( MGI:1915527)
Sequence:	sense strand is shown >T107 AGAATGGAGACCGCGATCGAAGACGCGGGACTGGACCGCGGCCCGACGCTGACCTCGTCCTGGGATGCGG CGTGCGGGGCGCTGACCCAAAGCCTTTTTCTTACCCGGACTGGGCCACGTGCCCAAGACTTGGACTTCGA GCAGCTACTGGAGCCGCCAGCTCCGAGCCAGGATCCGGTTAGTCTGAAAAGCAGCCTGAGCCCCCGAGAT GAAAACCCCTGCTTCATTTACCTGAACTGTGGCCCTAATGGGGGTGAAGAAATCCTTTCGGTTGGCGTTC TGAGTTCAGCAAGAAACATGGAAGTATACTTAGGAGAGGAGTATTGTGGAACCAGTCGAGGCAAGACTGC CTGCACTGTCCTGGATGACAGTGAACATGAAAAGATCTTGTTATACAAAAAATACCTAAAATTGGATTCT CCCACGCATGCCTGTAAAATAAAGCTGTTGTCCTTCGGGGAGAAGCAGTGTGTGCTTGTCAGTAAGGTGG TGGTGCACTTGA
Notes:	The probe template was PCR amplified from IMAGE:569956 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:569956 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18296 same embryo
	EMAGE:18297 same embryo
	EMAGE:18294 same embryo
	EMAGE:18293 same embryo
	EMAGE:18295 same embryo
	EurExpress:euxassay_012086 same experiment
	MGI:4822668 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source