Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18305

Ano1 anoctamin 1, calcium activated chloride channel ( MGI:2142149)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18305 EMAGE:18305 EMAGE:18305 EMAGE:18305 EMAGE:18305
"Pseudo-wholemount" of euxassay_012103. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012103_01 euxassay_012103_02 euxassay_012103_03 euxassay_012103_04
EMAGE:18305 EMAGE:18305 EMAGE:18305 EMAGE:18305 EMAGE:18305
euxassay_012103_05 euxassay_012103_06 euxassay_012103_07 euxassay_012103_08 euxassay_012103_09
EMAGE:18305 EMAGE:18305 EMAGE:18305 EMAGE:18305 EMAGE:18305
euxassay_012103_10 euxassay_012103_11 euxassay_012103_12 euxassay_012103_13 euxassay_012103_14
EMAGE:18305 EMAGE:18305 EMAGE:18305 EMAGE:18305 EMAGE:18305
euxassay_012103_15 euxassay_012103_16 euxassay_012103_17 euxassay_012103_18 euxassay_012103_19
EMAGE:18305 EMAGE:18305 EMAGE:18305 EMAGE:18305
euxassay_012103_20 euxassay_012103_21 euxassay_012103_22 euxassay_012103_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18305Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18305_wholemount_strong.wlz
18305_wholemount_moderate.wlz
18305_wholemount_weak.wlz
18305_wholemount_possible.wlz
18305_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18305_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 08 09 10 17 18 19
telencephalon mantle layer
strong strong
regionalstrong expression: see section 08 09 10 11 15 16 17 18
olfactory cortex ventricular layer
strong strong
regionalstrong expression: see section 10 11 15 16
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 12 13 14
rest of cerebellum ventricular layer
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18
cochlea
strong strong
regionalstrong expression: see section 09 18
utricle
strong strong
regionalstrong expression: see section 06 07 08 09 19 20 21 22 23
retina
strong strong
regionalstrong expression: see section 01 02 03 04 05 23
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 14 15 16 17 18
heart valve
strong strong
regionalstrong expression: see section 15 moderate expression: see section 16
stomach
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09
rectum
strong strong
regionalstrong expression: see section 12
midgut
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18 19
mandible
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23
maxilla
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 16 17 18 19 20 21 22 23
kidney calyx
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 16 17 18 19
kidney pelvis
moderate moderate
regionalmoderate expression: see section 17 18 19
ureter
moderate moderate
regionalmoderate expression: see section 09 10 11 15 16 17
left lung
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12
right lung
strong strong
regionalstrong expression: see section 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30726
Entity Detected:Ano1, anoctamin 1, calcium activated chloride channel ( MGI:2142149)
Sequence:sense strand is shown

>T30726
AGGGTGAGGCATGTTCCACAGACATATGGAGGAATATAGAGCCCCCCTGTGGTGGTAGGGAGCCAAGTCA
GCATCCTTAGTCTGAAGGATGCCCTGGGATAGATGCAGAATGGTACCTAGGAACCTGCTGACATTTTGCA
GAAGGGTGAACAACTCAGACTCAGATTCCCAAAGATGTCACCTGCTCTAGCTGGACAAGCTGGAAGACTC
CACTGTCTTTCCTTCTGGGAGCTCAGAAGTTCAGACCTGGGGTCAGAAACAGAGGTGCCTGACCACCCAC
ACAGCACACACACATGGTGGCTTCACATTGTACTCCAGTAACGGAGGAGTACAGGGGCTCCCAGTTGTCC
AGTACCCTTGGGGTCCCTGGCAGGCTGCACACAAGCCACTCAAGGGCCCCTCACATGAAGCTGATGTCCT
CCTTGTGAGACAAGCTGTCGTGGCAATCTCTAAACAGGCTGGAGGGCTTCACCAAGCCCTGGGGCCTCTG
TGGGTTCATTTTTTTTTTTTTTTGCTTTTTAAACCATCTATTGGTTTTTGTCTAAAAATCAATAATGTGA
TGGATTTGAGGGATTTTCTTTCTGTAGCTGAAGGTGGAAGGAGTTTATTTCATTAGTTAACCAAATACTG
AGAGGAAATTAAAATATCATTACTACCAAAGATTTTTCTCAATAAAAGCTTCTATTTTGGTAACACACCT
ATATTTTCACTCGTGGGGATGAGACCCTCAGCACTTACCATTGTAATAGCTTCTAAAACAAGCCTCATGG
GCCAGCTTCATTTGATGTGCTAGCCAGCTGTGCAAGGCCTCTCGGCACCATTGCCAGAAGTGTCTCTCTC
GCCCAAGCCGAAGAAGGCAATTCCCTGCACTCGTCTTAGGGCAAGGCAGACAGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3591765), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 14922. Forward Primer - name:014922_F_IRAV12-15_L15_AU040576, sequence:AGGGTGAGGCATGTTCCA; Reverse Primer - name:014922_R_SP6_IRAV12-15_L15_AU040576, sequence:CACTGTCTGCCTTGCCCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18306 same embryo
 EurExpress:euxassay_012103 same experiment
 MGI:4823141 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS