EMAGE mouse gene expression spatial database

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EMAGE:18314

Bcl9l B-cell CLL/lymphoma 9-like ( MGI:1933114)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012109_01	euxassay_012109_02	euxassay_012109_03	euxassay_012109_04	euxassay_012109_05

euxassay_012109_06	euxassay_012109_07	euxassay_012109_08	euxassay_012109_09	euxassay_012109_10

euxassay_012109_11	euxassay_012109_12	euxassay_012109_13	euxassay_012109_14	euxassay_012109_15

euxassay_012109_16	euxassay_012109_17	euxassay_012109_18	euxassay_012109_19	euxassay_012109_20

euxassay_012109_21	euxassay_012109_22	euxassay_012109_23	euxassay_012109_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18314_wholemount_strong.wlz

18314_wholemount_moderate.wlz

18314_wholemount_weak.wlz

18314_wholemount_possible.wlz

18314_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18314_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24

humerus

strong

regional

strong expression: see section 01 02 21 22 23 24

forelimb digit 1 phalanx

strong

regional

strong expression: see section 01

forelimb digit 2 phalanx

strong

regional

strong expression: see section 01

hindlimb digit 1 phalanx

strong

regional

strong expression: see section 08

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 07 08

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 06 07 08

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 07 08

fibula

strong

regional

strong expression: see section 01 02 03 04

tibia

strong

regional

strong expression: see section 01 02 03 04

femur

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 21 22 23 24

vibrissa

strong

spotted

strong expression: see section 24

otic capsule

strong

regional

strong expression: see section 06 07 08 16

naris

strong

regional

strong expression: see section 14 15 16 17 18 19

viscerocranium

strong

regional

Expression in the turbinate bone.

hyoid

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

meckel's cartilage

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21 22 23

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cricoid cartilage

strong

regional

strong expression: see section 10 11 12 13

thyroid cartilage

strong

regional

strong expression: see section 08 09 10 11 12 13 14

axial skeleton

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

basioccipital bone

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

basisphenoid bone

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

temporal bone petrous part

strong

regional

strong expression: see section 01 02 03 04 05 06 17 18 19 20 21 22 23 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 19 20 21 22 23 24

scapula

strong

regional

strong expression: see section 01 02 21 22

sternum

strong

regional

strong expression: see section 12 13

pelvic girdle skeleton

strong

regional

strong expression: see section 09 10 11 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30738
Entity Detected:	Bcl9l, B-cell CLL/lymphoma 9-like ( MGI:1933114)
Sequence:	sense strand is shown >T30738 CAAGCAGCACCCTCCAGTACTTCCCCAAGAGCGAGAACCAGCCCCCCAAGGCCCAGCCCCCCAATCTGCA TCTCATGAACCTGCAGAACATGATGGCAGAGCAGACCCCGTCTCGACCCCCCAACCTCCCGGGCCAACAG GGGGTCCAGCGGGGGCTCAGCATGTCCATGTGCCACCCTGGACAGATGTCCTTGCTGGGCAGGACAGGTG TGCCCCCACAACAGGGCATGGTGCCCCATGGCCTGCACCAGGGGGTCATGTCCCCTCCACAAGGCCTCAT GACCCAGCAGAATTTTATGCTGATGAAGCAGAGGGGTGTGGGGGGCGAGGTCTACACCCAGCCTCCCCAC ATGCTCTCCCCACAGGGCTCCCTCATGGGCCCCCCACCCCAGCAGAACCTCATGGTGTCCCACCCTCTGC GTCAGCGCAGTGTGTCTCTGGACAGCCAGATGGGCTACCTGCCAACGCCGGGCAGCATGGCCAATCTACC CTTCTAGCAGGCCCCTCGGTCTGGGGCTGGGGAAACTGAAAATATGTTAACTTTAAAAAGTTTTGTTTTT CCTCCAGTGTTGGGATGGATGGCCTGGGTCCAGGGGTAGGGTGGCGGGTGGGAACGGGCTTGTGTGGGGA GTGGTGTTTGTGGAAACCTGCTGTGCTGGCAGCTGAGGGGGAAGCGGCAAGTGTCGGGTGAGGAGTTGGA ATCGGGCTCTGCTCCATTTCTGTCACTAGGACTGCGCCTCCTCTGCCCCCCCCCCCCTTTCCCGTGGAGC TTCTGTCTCCCTTTGTCTTTGCACTTCCCCGCTTTCAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3155695), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13419. Forward Primer - name:013419_F_IRAV01-03_C22_Bcl9l, sequence:CAAGCAGCACCCTCCAGT; Reverse Primer - name:013419_R_SP6_IRAV01-03_C22_Bcl9l, sequence:AGCTGAAAGCGGGGAAGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18316 same embryo
	EMAGE:18317 same embryo
	EMAGE:18315 same embryo
	EMAGE:18313 same embryo
	EMAGE:18312 same embryo
	EurExpress:euxassay_012109 same experiment
	MGI:4823454 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source