EMAGE mouse gene expression spatial database

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EMAGE:18319

Cdh1 cadherin 1 ( MGI:88354)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012131. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012131_01	euxassay_012131_02	euxassay_012131_03	euxassay_012131_04

euxassay_012131_05	euxassay_012131_06	euxassay_012131_07	euxassay_012131_08	euxassay_012131_09

euxassay_012131_10	euxassay_012131_11	euxassay_012131_12	euxassay_012131_13	euxassay_012131_14

euxassay_012131_15	euxassay_012131_16	euxassay_012131_17	euxassay_012131_18	euxassay_012131_19

euxassay_012131_20	euxassay_012131_21	euxassay_012131_22	euxassay_012131_23	euxassay_012131_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18319_wholemount_strong.wlz

18319_wholemount_moderate.wlz

18319_wholemount_weak.wlz

18319_wholemount_possible.wlz

18319_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18319_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 17 18 19 20 21 22 23 24

upper leg muscle

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 02 03 04

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 14

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 15 16

spinal cord

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13

retina

strong

regional

strong expression: see section 01 02 03 04 21

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 17 18

vomeronasal organ

strong

regional

strong expression: see section 12 15

heart ventricle

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15

esophagus

strong

regional

strong expression: see section 09 10

tongue extrinsic muscle

strong

regional

strong expression: see section 10 11 12 13

tongue intrinsic muscle

strong

regional

strong expression: see section 12

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09

midgut

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36138
Entity Detected:	Cdh1, cadherin 1 ( MGI:88354)
Sequence:	sense strand is shown >T36138 TCTGTTGGTGTTCGATTACGAGGGCAGTGGTTCTGAAGCCGCTAGCCTGAGCTCACTGAACTCCTCTGAG TCGGATCAGGACCAGGACTACGATTATCTGAACGAGTGGGGCAACCGATTCAAGAAGCTGGCGGACATGT ACGGCGGTGGTGAGGACGACTAGGGGACTAGCAAGTCTCCCCCGTGTGGCACCATGGGAGATGCAGAATA ATTATATCAGTGGTCTTTCAGCTCCTTCCCTGAGTGTGTAGAAGAGAGACTGATCTGAGAAGTGTGCAGA TTGCATAGTGGTCTCACTCTCCCTACTGGACTGTCTGTGTTAGGATGGTTTTCACTGATTGTTGAAATCT TTTTTTATTTTTTATTTTTACAGTGCTGAGATATAAACTGTGCCTTTTTTTGTTTGTTTGTTTCTGTTTT TGTTCTTTTGAGCTATGATCTGCCCCAGACACAACAGCCCCAAGCCCCTCACACCTCACTAATTTTTTAC ATTGTGTACTTGCCCTCAATTACCATGTTTGCTGTATTCTAATAGTCACTCATGTTCCTGAATTCTGTTG CCCTGCCCAGGTGATATTCTAGGATGCAGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89831. Forward Primer - name:089831_F_cDNA_Cdh1, sequence:TCTGTTGGTGTTCGATTACGAG; Reverse Primer - name:089831_N_SP6_cDNA_Cdh1, sequence:TCTGCATCCTAGAATATCACCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18321 same embryo
	EMAGE:18324 same embryo
	EMAGE:18320 same embryo
	EMAGE:18322 same embryo
	EMAGE:18323 same embryo
	EurExpress:euxassay_012131 same experiment
	MGI:4823761 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source