EMAGE mouse gene expression spatial database

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EMAGE:18320

Esam endothelial cell-specific adhesion molecule ( MGI:1916774)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012134. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012134_01	euxassay_012134_02	euxassay_012134_03	euxassay_012134_04

euxassay_012134_05	euxassay_012134_06	euxassay_012134_07	euxassay_012134_08	euxassay_012134_09

euxassay_012134_10	euxassay_012134_11	euxassay_012134_12	euxassay_012134_13	euxassay_012134_14

euxassay_012134_15	euxassay_012134_16	euxassay_012134_17	euxassay_012134_18	euxassay_012134_19

euxassay_012134_20	euxassay_012134_21	euxassay_012134_22	euxassay_012134_23	euxassay_012134_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18320_wholemount_strong.wlz

18320_wholemount_moderate.wlz

18320_wholemount_weak.wlz

18320_wholemount_possible.wlz

18320_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18320_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

other

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4742
Entity Detected:	Esam, endothelial cell-specific adhesion molecule ( MGI:1916774)
Sequence:	sense strand is shown >T4742 CCCTGGTCAGTAGCCTGGTTCCTGTGCACATTCGGGTTGCCGCCGCTCACCCACAACACCTGTAGACACC GTGTGTCCAACTCTCCCTGAGTACTCCGGGCCAAGGAGGGCCATGATTCTTCAGGCTGGAACCCCCGAGA CCAGCTTGCTGCGGGTTTTGTTCCTGGGACTGAGTACCCTTGCTGCCTTCTCCCGAGCTCAGATGGAGTT GCACGTGCCCCCGGGCCTCAACAAATTGGAAGCGGTAGAGGGAGAAGAAGTGGTGCTCCCCGCCTGGTAC ACGATGGCACGGGAGGAGTCGTGGTCCCACCCCCGGGAGGTGCCCATCCTGATCTGGTTCTTGGAACAAG AAGGGAAGGAACCAAACCAGGTGTTGTCTTACATTAATGGAGTCATGACAAATAAACCTGGAACAGCCCT GGTCCACTCTATCTCTTCACGGAATGTGTCCCTGCGCCTGGGGGCACTCCAGGAGGGAGACTCTGGGACT TACCGCTGTTCTGTCAATGTGCAGAATGATGAAGGCAAAAGTATAGGCCACAGCATCAAAAGCATAGAGC TCAAAGTGCTGGTTCCTCCAGCTCCTCCATCCTGTAGTTTACAGGGTGTACCCTATGTCGGGACCAATGT GACCCTGAACTGCAAGTCCCCAAGGAGTAAACCTACTGCTCAGTACCAGTGGGAGAGGCTGGCCCCATCC TCCCAGGTCTTCTTTGGACCAGCCTTAGATGCTGTTCGTGGATCTTTAAAGCTCACTAACCTTTCCATTG CCATGTCTGGAGTCTATGTCTGCAAGGCTCAAAACAGAGTGGGCTTTGCCAAGTGCAACGTGACCTTGGA CGTGATGACAGGGTCCAAGGCTGCAGTGGTCGCTGGAGCAGTTGTGGGCACTTTTGTTGGGTTGGTGCTG ATAGCTGGGCTGGTCCTGTTGTACCAGCGCCGGAGCAAGACCTTGGAAGAGCTGGCCAATGATATCAAGG AAGATGCCATTGCTCCCCGGACCTTGCCTTGGACCAAAGGCTCAGACACAATCTCCAAGAATGGGACACT TTCTTCGGTCACCTCAGCACGAGCTCTGCGGCCACCCAAGGCTGCTCCTCCAAGACCTGGCACATTTACT CCCACACCCAGTGTCTCTAGCCAGGCCCTGTCCTCACCAAGACTGCCCAGGGTAGATGAACCCCCACCTC AGGCAGTGTCCCTGACCCCAGGTGGGGTTTCTTCTTCTGCTCTGAGCCGCATGGGTGCTGTGCCTGTGAT GGTGCCTGCACAGAGTCAGGCTGGGTCTCTTGTGTGATAGCCCAGGCACTCATTAGCTACATCTGGTATC TGACCTTTCTGTAAAGGTCTCCTTGTGGCACAGAGGACTCAATCTTGGGAGGATGCCCACATTCTAGACC TCCAGTCCTTTGCTCCTACCTCCTTCTATTGTTGGAATACTGGGCCTCAGTAAGACTAAAATCTGGGTCA AAGGACAAAAGGAGGAAATGGACCTGAGGTAGGGGGTTGGGAGTAAGGAGGCTTCACTTCCTCCCTGCTT CTCCCTGAAGCCAGATGAATGCTGCGGAAGATCGGCTACCCTCCAAGGGCTCTGGAGGAGACTGCCAGTC AGTGATGCCCCTGGCTCTGTGATCTGTACAACACCCTTATCTAATGCTGTCCTTTGCCGTTCGCTCCATC TCCCTGTATTAATATAACCTGTCCTGCTGGCTTGGCTGGGTTTTGTTGTAGCAGGGGGATAGGAAAGACA TTTTAAAACCTGACTTGAAATTGATGTTTTTGTTTTTATTTTGCAAATTTCAATAAAGATACATCGCATT TGCATGGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5039892 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5039892 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18321 same embryo
	EMAGE:18324 same embryo
	EMAGE:18319 same embryo
	EMAGE:18322 same embryo
	EMAGE:18323 same embryo
	EurExpress:euxassay_012134 same experiment
	MGI:4824622 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source