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EMAGE:18325

Unc5cl unc-5 homolog C (C. elegans)-like ( MGI:1923839)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18325 EMAGE:18325 EMAGE:18325 EMAGE:18325 EMAGE:18325
"Pseudo-wholemount" of euxassay_012140. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012140_01 euxassay_012140_02 euxassay_012140_03 euxassay_012140_04
EMAGE:18325 EMAGE:18325 EMAGE:18325 EMAGE:18325 EMAGE:18325
euxassay_012140_05 euxassay_012140_06 euxassay_012140_07 euxassay_012140_08 euxassay_012140_09
EMAGE:18325 EMAGE:18325 EMAGE:18325 EMAGE:18325 EMAGE:18325
euxassay_012140_10 euxassay_012140_11 euxassay_012140_12 euxassay_012140_13 euxassay_012140_14
EMAGE:18325 EMAGE:18325 EMAGE:18325 EMAGE:18325 EMAGE:18325
euxassay_012140_15 euxassay_012140_16 euxassay_012140_17 euxassay_012140_18 euxassay_012140_19
EMAGE:18325 EMAGE:18325 EMAGE:18325 EMAGE:18325
euxassay_012140_20 euxassay_012140_21 euxassay_012140_22 euxassay_012140_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18325Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18325_wholemount_strong.wlz
18325_wholemount_moderate.wlz
18325_wholemount_weak.wlz
18325_wholemount_possible.wlz
18325_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18325_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 14 16 17 18 20 21 22 moderate expression: see section 03 19 weak expression: see section 02
radius
strong strong
regionalstrong expression: see section 01
humerus
strong strong
regionalstrong expression: see section 01 02 03 04 18 19 20 21 22 23
fibula
strong strong
regionalstrong expression: see section 02
tibia
strong strong
regionalstrong expression: see section 01 02
femur
strong strong
regionalstrong expression: see section 04 05 06 07 08 19 20 21 22 23 moderate expression: see section 18
otic capsule
strong strong
regionalstrong expression: see section 08 15 weak expression: see section 06 07
naris
strong strong
regionalstrong expression: see section 15 moderate expression: see section 11
nasal septum
moderate moderate
regionalmoderate expression: see section 12
viscerocranium
strong strong
regionalExpression in the turbinate bone.
hyoid
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14
meckel's cartilage
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 15 16 17 18 19 20 moderate expression: see section 03 04 13 14 weak expression: see section 11 12
axial skeleton
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16
basioccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
basisphenoid bone
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15
temporal bone petrous part
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 18 19 20 21
orbito-sphenoid
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 16 17 18 19 20 21 22
scapula
strong strong
regionalstrong expression: see section 03 04 19 20
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 09 16 moderate expression: see section 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31460
Entity Detected:Unc5cl, unc-5 homolog C (C. elegans)-like ( MGI:1923839)
Sequence:sense strand is shown

>T31460
CCTCCCACCTGGGTCTCTGTGGCATGAAAATCCGGTTCTTGTCCTGCCAGCGCAGTCCCGCCGCGGCCAT
TCTGGAATTGTTCGAGGAACAGAATGGCAGCTTGCAGGAGCTGCACTATCTCATGACCTCCATGGAGCGG
CTGGACTGCGCCTCTGCCATCCAGAACTACCTAAACCGGTCTCCCCGGGGCAGCCCTGACAGGTTGCATG
GAGGCACCTGGGAGAACCATGGCCTAGAGCTGGATGAGAAACTCTAAGCAGACTCCACGGCTGTGCAGGG
TGCTTCAAGAGGCCATGCCATACCGTGCCATGCCTTTGAGCTCAGGATCAATGCCACAGTCTCAGGTGGA
CCGAACTGGAACCCGCAGCAAAGCCCGACAGCCCTGTCATCTGCCCTGCATCAGGATAGCTCTTAATCCT
GCCGGCCAACTTTGTCCTGCGTGCAGATTCCTTCTTGGTCGGTAGGGGGCGCGGGAGTCTGGGAGATGGT
CACAGCCAAGCAGCTTCTGAGATGCTGCTTAATTTTCTTGTGGACCTGTGGGCAGACCTCAGAGACTGCT
CAGTCCACAAATATTCATGGAGCGCCGAGTCTCAGCACCAAGCTGGGATCATGAGGAACGTCGGCTTCTT
TGGACTTGTCTTGGAGTCTGTTCTGCGAAATGAAGATGCACTCCCAGCCGGCTGAGAGCAACATTCTAGA
CAGTGTCTCCCTGTGGTTAGGAGATGTCTTGGGTGTGTAACTTTGTTGAGAAGGGAGGGCCCTGCGGTGG
ACTGGAACAAAGAGAAGTCTCTATAGAGGGTCTTGGGAGAGAACTTTGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4482683), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26683. Forward Primer - name:026683_F_IRAV59-62_L10_Unc5cl, sequence:CCTCCCACCTGGGTCTCT; Reverse Primer - name:026683_R_SP6_IRAV59-62_L10_Unc5cl, sequence:CGCCAAAGTTCTCTCCCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18326 same embryo
 EMAGE:18330 same embryo
 EMAGE:18327 same embryo
 EMAGE:18329 same embryo
 EMAGE:18328 same embryo
 EurExpress:euxassay_012140 same experiment
 MGI:4829103 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS