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EMAGE:18330

Gaa glucosidase, alpha, acid ( MGI:95609)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18330 EMAGE:18330 EMAGE:18330 EMAGE:18330 EMAGE:18330
"Pseudo-wholemount" of euxassay_012135. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012135_01 euxassay_012135_02 euxassay_012135_03 euxassay_012135_04
EMAGE:18330 EMAGE:18330 EMAGE:18330 EMAGE:18330 EMAGE:18330
euxassay_012135_05 euxassay_012135_06 euxassay_012135_07 euxassay_012135_08 euxassay_012135_09
EMAGE:18330 EMAGE:18330 EMAGE:18330 EMAGE:18330 EMAGE:18330
euxassay_012135_10 euxassay_012135_11 euxassay_012135_12 euxassay_012135_13 euxassay_012135_14
EMAGE:18330 EMAGE:18330 EMAGE:18330 EMAGE:18330 EMAGE:18330
euxassay_012135_15 euxassay_012135_16 euxassay_012135_17 euxassay_012135_18 euxassay_012135_19
EMAGE:18330
euxassay_012135_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18330Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18330_wholemount_strong.wlz
18330_wholemount_moderate.wlz
18330_wholemount_weak.wlz
18330_wholemount_possible.wlz
18330_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18330_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 12 13 14 15 16 17 18 19 20
humerus
strong strong
regionalstrong expression: see section 01 02 16 17 18 19 20
femur
strong strong
regionalstrong expression: see section 01 02 03 04 05 15 16 17 18 19 20
otic capsule
strong strong
regionalstrong expression: see section 05 12 13
naris
strong strong
regionalstrong expression: see section 07 08 11 12 13
viscerocranium
strong strong
regionalExpression in the turbinate bone.
hyoid
strong strong
regionalstrong expression: see section 06 08 09 11 12 weak expression: see section 07
meckel's cartilage
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
axial skeleton
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14
basioccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 19
basisphenoid bone
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13
temporal bone petrous part
strong strong
regionalstrong expression: see section 01 02 14 15 16 17 18 19
vault of skull
strong strong
regionalstrong expression: see section 01 02
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 15 16 18 19 20
clavicle
strong strong
regionalstrong expression: see section 03 04 05 06 13 14
scapula
strong strong
regionalstrong expression: see section 01 02 17 18 19
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 06 07 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30063
Entity Detected:Gaa, glucosidase, alpha, acid ( MGI:95609)
Sequence:sense strand is shown

>T30063
TACCCCTTCATGCCTCCATACTGGGGCCTCGGCTTCCACCTCTGCCGCTGGGGCTACTCCTCGACCGCCA
TTGTCCGCCAGGTAGTGGAGAACATGACCAGGACACACTTCCCGCTGGATGTGCAATGGAATGACCTGGA
CTACATGGACGCCCGAAGAGACTTCACCTTCAACCAGGACAGCTTTGCCGACTTCCCAGACATGGTGCGG
GAGCTGCACCAGGGTGGCCGGCGCTACATGATGATCGTGGACCCTGCCATCAGCAGCGCAGGCCCTGCTG
GGAGTTACAGGCCCTACGACGAGGGTCTGCGGAGGGGTGTGTTCATCACCAACGAGACTGGGCAGCCGCT
GATTGGGAAGGTTTGGCCCGGAACCACCGCCTTCCCTGATTTCACCAACCCTGAGACCCTTGACTGGTGG
CAGGACATGGTGTCTGAGTTCCACGCCCAGGTGCCCTTCGATGGCATGTGGCTCGACATGAACGAACCGT
CCAACTTCGTTAGAGGCTCTCAGCAGGGCTGCCCCAACAATGAACTGGAGAACCCCCCCTATGTGCCCGG
GGTGGTTGGCGGGATCTTGCAGGCAGCCACCATCTGTGCCTCCAGCCACCAATTCCTCTCCACACACTAC
AACCTCCACAACCTGTACGGCCTCACTGAAGCTATCGCCTCCAGCAGGGCCCTGGTCAAGACTCGGGGAA
CACGACCCTTTGTGATCTCCCGCTCAACCTTCTCGGGCCACGGCCGGTACGCTGGTCACTGGACAGGGGA
TGTGCGGAGCTCTTGGGAGCATCTTGCATACTCTGTGCCAGACATCCTGCAGTTCAACCTGCTGGGCGTG
CCCCTGGTCGGGGCGGACATCTGCGGCTTCATAGGAGACACGTCAGAAGAGCTGTGTGTGCGCTGGACCC
AGTTGGGGGCCTTCTACCCCTTCATGCGGAACCACAATGACCTGAATAGCGTGCCTCAGGAGCCGTACAG
GTTCAGCGAGACG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3969127), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15255. Forward Primer - name:015255_F_IRAV20-23_C17_Gaa, sequence:TACCCCTTCATGCCTCCA; Reverse Primer - name:015255_R_SP6_IRAV20-23_C17_Gaa, sequence:CCGTCTCGCTGAACCTGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18326 same embryo
 EMAGE:18327 same embryo
 EMAGE:18329 same embryo
 EMAGE:18325 same embryo
 EMAGE:18328 same embryo
 EurExpress:euxassay_012135 same experiment
 MGI:4824959 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS